Growth media - Central Collection of Algal Cultures (CCAC)

Soil-Water Medium

 

Soil-Water Medium G

Place 1 cm of garden soil in the bottom of a test tube and a) fill up with 10 ml of dH2O; or b) add ½ wheat grain (presoaked) and fill up with 10 ml of dH2O; or c) place a small pinch of NH4MgPO4 in the bottom of the test tube, add 1 cm garden soil and fill up with 10 ml of dH2O.

Cover with a cotton plug or a metal cap. After soaking for at least 12 h autoclave for 30 minutes at 121°C.

 

Soil-Water Medium H

Place 1 cm of soil taken from an area vegetated with hornbeam in the bottom of a test tube and a) fill up with 10 ml of dH2O; or b) add ½ wheat grain (presoaked) and fill up with 10 ml of dH2O; or c) place a small pinch of NH4MgPO4 in the bottom of the test tube, add 1 cm garden soil and fill up with 10 ml of dH2O.

Cover with a cotton plug or a metal cap. After soaking for at least 12 h autoclave for 30 minutes at 121°C.

 

 

Growth Conditions
If not otherwise stated, growth temperature is +/- 15oC; light-dark-regime 14:10 h; light-intensity 5-15 µmol photons /m2/sec. Media are made up to 1 litre with dH2O or filtered seawater, as indicated.