ZMB Member Shirley Knauer, Publications

Prof. Dr. Shirley Knauer

Selected Publications

ISI Researcher ID E-7931-2012

Original Research Papers

Westmeier D, Solouk-Saran D, Vallet C, Siemer S, Docter D, Götz H, Männ L, Hasenberg A, Hahlbrock A, Erler K, Reinhardt C, Schilling O, Becker S, Gunzer M, Hasenberg M, Knauer SK, Stauber RH. Nanoparticle decoration impacts airborne fungal pathobiology. Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):7087-7092.

Knauer SK, Mahendrarajah N, Roos WP, Krämer OH. The inducible E3 ubiquitin ligases SIAH1 and SIAH2 perform critical roles in breast and prostate cancers. Cytokine Growth Factor Rev. 2015 May 12. pii: S1359-6101(15)00031-3.

Wünsch D, Hahlbrock A, Heiselmayer C, Bäcker S, Schrenk C, Benne F, Schilling O, Knauer SK. Evolutionary divergence of Threonine Aspartase1 leads to species-specific substrate recognition. Biol Chem. 2015 Apr;396(4):367-76.

Wagner T, Kiweler N, Wolff K, Knauer SK, Brandl A, Hemmerich P, Dannenberg JH, Heinzel T, Schneider G, Krämer OH. Sumoylation of HDAC2 promotes NF-κB-dependent gene expression. Oncotarget. 2015 Mar 30;6(9):7123-35.

Docter D, Strieth S, Westmeier D, Hayden O, Gao M, Knauer SK, Stauber RH. No king without a crown--impact of the nanomaterial-protein corona on nanobiomedicine. Nanomedicine (Lond). 2015 Feb;10(3):503-19.

Wünsch D, Hahlbrock A, Heiselmayer C, Bäcker S, Heun P, Goesswein D, Stöcker W, Schirmeister T, Schneider G, Krämer OH, Knauer SK, Stauber RH. Fly versus man: Evolutionary impairment of nucleolar targeting affects the degradome of Drosophila's Taspase1. FASEB J. 2015 Jan 29.

Li M, Schlesiger S, Knauer SK, Schmuck C. A Tailor-Made Specific Anion-Binding Motif in the Side Chain Transforms a Tetrapeptide into an Efficient Vector for Gene Delivery. Angew Chem Int Ed Engl. 2015 Jan 22.

van den Boom J, Mamić M, Baccelliere D, Zweerink S, Kaschani F, Knauer S, Bayer P, Kaiser M. Peptidyl Succinimidyl Peptides as Taspase 1 Inhibitors. Chembiochem. 2014 Aug 21.

Docter D, Distler U, Storck W, Kuharev J, Wünsch D, Hahlbrock A, Knauer SK, Tenzer S, Stauber RH. Quantitative profiling of the protein coronas that form around nanoparticles. Nat Protoc. 2014 Sep;9(9):2030-44.

Althoff K, Lindner S, Odersky A, Mestdagh P, Beckers A, Karczewski S, Molenaar JJ, Bohrer A, Knauer S, Speleman F, Epple M, Kozlova D, Yoon S, Baek K, Vandesompele J, Eggert A, Schramm A, Schulte JH. miR-542-3p exerts tumor suppressive functions in neuroblastoma by downregulating Survivin. Int J Cancer. 2014 Jul 21.

Kuchelmeister HY, Karczewski S, Gutschmidt A, Knauer S, Schmuck C. Utilizing combinatorial chemistry and rational design: peptidic tweezers with nanomolar affinity to DNA can be transformed into efficient vectors for gene delivery by addition of a lipophilic tail. Angew Chem Int Ed Engl. 2013 Dec 23;52(52):14016-20.

Tenzer S, Docter D, Kuharev J, Musyanovych A, Fetz V, Hecht R, Schlenk F, Fischer D, Kiouptsi K, Reinhardt C, Landfester K, Schild H, Maskos M, Knauer SK, Stauber RH. Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. Nat Nanotechnol. 2013 Oct;8(10):772-81.

Knauer SK, Unruhe B, Karczewski S, Hecht R, Fetz V, Bier C, Friedl S, Wollenberg B, Pries R, Habtemichael N, Heinrich UR, Stauber RH. Functional characterization of novel mutations affecting Survivin (BIRC5)-mediated therapy resistance in head and neck cancer patients. Human Mutation. 2012

Kuchelmeister HY, Tillmann S, Gutschmidt A, Knauer SK, Schmuck C. Efficient Gene Delivery into Cells by a Surprisingly Small Three-Armed Peptide Ligand. Chem. Sci. 2012, 3: 996-1002

Schröder E, Gebel L, Eremeev A, Morgner J, Grum D, Knauer SK, Bayer P, Müller JW. Human PAPS synthase isoforms are dynamically regulated enzymes with access to nucleus and cytoplasm. PLOS One. 2012, 7(1):e29559 (epub).

Stauber RH, Knauer SK, Habtemichael N, Bier C, Unruhe B, Weisheit S, Spange S, Nonnenmacher F, Fetz V, Ginter T, Reichardt S, Liebmann C, Schneider G, Krämer OH. A combination of a ribonucleotide reductase inhibitor and histone deacetylase inhibitors downregulates EGFR and triggers BIM- dependent apoptosis in head and neck cancer. Oncotarget. 2012, 3(1):31-43.

Brandl A, Wagner T, Uhlig KM, Knauer SK, Stauber RH, Melchior F, Schneider G, Heinzel T, Krämer OH. Dynamically regulated sumoylation of HDAC2 controls p53 deacetylation and restricts apoptosis following genotoxic stress. J Mol Cell Biol. 2012 Oct;4(5):284-93.

Krämer OH, Stauber RH, Bug G, Hartkamp J, Knauer SK. SIAH proteins: Critical roles in leukemogenesis. Leukemia. 2012 Oct 5. doi: 10.1038/leu.2012.284. [Epub ahead of print]

Wünsch D, Fetz V, Heider D, Tenzer S, Bier C, Kunst L, Knauer SK, and Stauber, RH. (2012). Chemico-genetic strategies to inhibit the leukemic potential of Threonin-Aspartase-1. Blood Cancer Journal (in press).

Bier C, Knauer SK, Wünsch D, Kunst L, Scheiding S, Kaiser M, Ottmann C, Krämer OH, Stauber RH. Allosteric inhibition of Taspase1's pathobiological activity by enforced dimerization in vivo. FASEB J.  2012 Aug;26(8):3421-9.

Stauber RH, Bier C, Knauer SK. Targeting taspase1 for cancer therapy--letter. Cancer Res. 2012 Jun 1;72(11):2912. Epub 2012 May 16.

Gebauer JS, Malissek M, Simon S, Knauer SK, Maskos M, Stauber RH, Peukert W, Treuel L. Impact of the Nanoparticle-Protein Corona on Colloidal Stability and Protein Structure. Langmuir. 2012 May 8. [Epub ahead of print]

Bier C, Hecht R, Kunst L, Scheiding S, Wünsch D, Goesswein D, Schneider G, Krämer OH, Knauer SK, Stauber RH. Overexpression of the Catalytically Impaired Taspase1(T234V) or Taspase1(D233A) Variants Does Not Have a Dominant Negative Effect in T(4;11) Leukemia Cells. PLoS One. 2012;7(5):e34142. Epub 2012 May 3.

Ginter T, Bier C, Knauer SK, Sughra K, Hildebrand D, Münz T, Liebe T, Heller R, Henke A, Stauber RH, Reichardt W, Schmid JA, Kubatzky KF, Heinzel T, Krämer OH. Histone deacetylase inhibitors block IFN?-induced STAT1 phosphorylation. Cell Signal. 2012 Jul;24(7):1453-60. Epub 2012 Mar 7.

Pietschmann K, Buchwald M, Müller S, Knauer SK, Kögl M, Heinzel T, Krämer OH. Differential regulation of PML-RAR? stability by the ubiquitin ligases SIAH1/2 and TRIAD1. Int J Biochem Cell Biol. 2012 Jan;44(1):132-8. Epub 2011 Oct 22.

Tenzer S, Docter D, Rosfa S, Wlodarski A, Kuharev J, Rekik A, Knauer SK, Bantz C, Nawroth T, Bier C, Sirirattanapan J, Mann W, Treuel L, Zellner R, Maskos M, Schild H, Stauber RH. Nanoparticle size is a critical physicochemical determinant of the human blood plasma corona - A comprehensive quantitative proteomic analysis. ACS Nano 2011, Sep 27;5(9):7155-67. Epub 2011 Aug 25.

Knauer SK, Fetz V, Rabenstein J, Friedl S, Hofmann B, Sabiani S, Schröder E, Kunst L, Proschak E, Thines E, Kindler T, Schneider G, Marschalek R, Stauber RH, Bier C. Bioassays to monitor taspase1 function for the identification of pharmacogenetic inhibitors. PLoS One. 2011;6(5):e18253. doi:10.1371/journal.pone.0018253, epub 2011 May 25.

Bier C, Knauer SK, Docter D, Schneider G, Krämer OH, Stauber RH. The importin-alpha/nucleophosmin switch controls taspase1 protease function. Traffic. 2011 Jun;12(6):703-14. doi: 10.1111/j.1600-0854.2011.01191.x. Epub 2011 Apr 13.

Pless B, Oehm C, Knauer S, Stauber RH, Dingermann T, Marschalek R. The heterodimerization domains of MLL - FYRN and FYRC - are potential target structures in t(4;11) leukemia. Leukemia. 2011 Apr;25(4):663-70. Epub 2011 Jan 14.

Bier C, Knauer SK, Klapthor A, Schweitzer A, Rekik A, Krämer OH, Marschalek R, Stauber RH. Cell-based analysis of structure-function activity of Threonine Aspartase 1. J Biol Chem. 2011 Jan 28;286(4):3007-17. Epub 2010 Nov 17.

Knauer SK, Heinrich UR, Bier C, Habtemichael N, Docter D, Helling K, Mann WJ, Stauber RH. An otoprotective role for the apoptosis inhibitor protein survivin. Cell Death Dis. 2010 Jul 1;1:e51.

Habtemichael N, Wünsch D, Bier C, Tillmann S, Unruhe B, Frauenknecht K, Heinrich UR, Mann WJ, Stauber RH, Knauer SK. Cloning and functional characterization of the guinea pig apoptosis inhibitor protein Survivin. Gene. 2010 Dec 1;469(1-2):9-17. Epub 2010 Aug 19.

Habtemichael N, Heinrich UR, Knauer SK, Schmidtmann I, Bier C, Docter D, Brochhausen C, Helling K, Brieger J, Stauber RH, Mann WJ. Expression analysis suggests a potential cytoprotective role of Birc5 in the inner ear. Mol Cell Neurosci. 2010 Nov;45(3):297-305. Epub 2010 Jul 17.

Schweitzer A,Knauer SK, Stauber RH. Nuclear receptors in head and neck cancer: current knowledge and perspectives. Int J Cancer. 2010 Feb 15;126(4):801-9.

Knauer SK. Prognostic and therapeutic potential of nuclear receptors in head and neck squamous cell carcinomas. J Oncol. 2009;2009:349205. Epub 2009 Sep 24.

Fetz V, Knauer SK, Bier C, v. Kries JP and Stauber RH. Translocation biosensors – cellular system integrators to dissect CRM1-dependent nuclear export by chemicogenomics. Sensors, 2009, 9(7):5423-45.

Krämer OH, Knauer SK, Greiner G, Jandt E, Reichardt S, Gührs KH, Stauber RH, Böhmer FD and Heinzel T. A phosphorylation-acetylation switch regulates STAT1 signaling. Genes Dev, 2009, 23(2):223-35.

Fetz V, Bier C, Habtemichael N, Schuon R, Schweitzer A, Kunkel M, Engels K, Kovács AF, Schneider S, Mann W, Stauber RH and Knauer SK. Inducible NO synthase confers chemoresistance in head and neck cancer by modulating survivin. Int J Cancer, 2009, 124(9):2033-41.

Pautz A, Linker K, Altenhöfer S, Heil S, Schmidt N, Art J, Knauer SK, Stauber RH, Sadri N, Pont A, Schneider RJ and Kleinert H. Similar regulation of human inducible nitric oxide synthase (iNOS) expression by different isoforms of the RNA binding protein AUF1. J Biol Chem, 2009, 284(5):2755-66.

Engels K, Knauer SK, Loibl S, Fetz V, Harter P, Schweitzer A, Fisseler-Eckhoff A, Kommoss F, Hanker L, Nekljudova V, Hermanns I, Kleinert H, Mann W, du Bois A and Stauber RH. NO signaling confers cytoprotectivity through the survivin network in ovarian carcinomas. Cancer Res, 2008, 68(13):5159-66.

Krämer OH, Zimmermann D, Knauer SK, Stauber RH and Heinzel T. Histone deacetylase inhibitors and hydroxyurea modulate the cell cycle and cooperatively induce apoptosis. Oncogene, 2008, 27(6):732-40.

Knauer SK, Bier C, Schlag P, Dietmaier W, Rödel F, Klein-Hitpass L, Kovács AF, Döring C, Hansmann ML, Lippert B, Hofmann WK, Kunkel M, Brochhausen C, Engels K, Mann W and Stauber RH. The survivin splice variant survivin-3B is cytoprotective and can function as a chromosomal passenger complex protein. Cell Cycle, 2007, 6(12):1502-9.

Seil I, Frei C, Sültmann H, Engels K, Knauer SK, Jäger E, Zatloukal K, Knuth A, Moch H, Varga Z, Jungbluth A, Pfreundschuh M, Old LJ, Chen YT, Jäger D and Stauber RH. The differentiation antigen NY-BR-1 is a potential target for antibody based therapies in breast cancer. Int J Cancer, 2007, 120(12):2635-42.Knauer SK, Krämer OH, Knösel T, Engels K, Rödel F, Kovács AF, Dietmaier W, Klein-Hitpass L, Habtemichael N, Schweitzer A, Brieger J, Rödel C, Mann W, Petersen I, Heinzel T and Stauber RH. Nuclear export is essential for the tumor promoting activity of survivin. FASEB J, 2007, 21(1):207-16.

Engels K, Knauer SK, Metzler D, Simf C, Struschka O, Bier C, Mann W, Hansmann ML, Kovács AF and Stauber RH. Dynamic Intracellular Survivin as an Early Prognostic Marker in Oral Squamous Cell Carcinomas – Underlying Molecular Mechanism and Potential. J Pathol, 2007, 211(5):532-40.

Knauer SK, Bier C, Habtemichael N and Stauber RH. The Survivin-Crm1 interaction is essential for chromosomal passenger complex localization and function. EMBO Rep, 2006, 7(12):1259-65.

Krämer OH, Baus D, Knauer SK, Stein S, Jäger E, Stauber RH, Grez M, Pfitzner E and Heinzel T. Acetylation of Stat1 modulates NF-kB activity. Genes Dev, 2006, 20(4):473-85.

Dälken B, Giesübel U, Knauer SK and Wels WS. Targeted induction of apoptosis by chimeric granzyme B fusion proteins carrying antibody and growth factor domains for cell recognition. Cell Death Differ, 2005, 13(4):576–585.

Knauer SK, Moodt S, Berg T, Liebel U, Pepperkok R and Stauber RH. Translocation Biosensors To Study Signal Specific Nucleo-Cytoplasmic Transport, Protease Activity & Protein Interactions. Traffic, 2005, 6(7):594-606.

Knauer SK, Carra G and Stauber RH. Nuclear Export Is Evolutionarily Conserved in CVC Paired-Like Homeobox Proteins and Influences Protein Stability, Transcriptional Activation, and Extracellular Secretion. Mol Cell Biol, 2005, 25(7):2573-82.


Schweitzer A, Knauer SK and Stauber RH. Nuclear receptors in head and neck cancer: Current knowledge and perspectives. Int J Cancer, 2010, 126(4):801-9.

Schweitzer A, Knauer SK and Stauber RH. Therapeutic potential of nuclear receptors. Expert Opinion on Therapeutic Patents, 2008, 18(8):861-88.

Capalbo G, Rödel C, Stauber RH, Knauer SK, Bache M, Kappler M and Rödel F. The Role of Survivin for Radiation Therapy: Prognostic and Predictive Factor and Therapeutic Target. Strahlentherapie und Onkologie, 2007, 183(11):593-9.

Stauber RH, Mann W and Knauer SK. Nuclear and Cytoplasmic Survivin: Molecular Mechanism, Prognostic, and Therapeutic Potential. Cancer Res, 2007, 67(13):5999-6002.

Lippert BM, Knauer SK, Fetz V, Mann W and Stauber RH. Double duty of dynamic survivin in head and neck cancer: Molecular mechanism and therapeutic potential. Int J Cancer, 2007, 121(6):1169-74.

Knauer SK, Mann W and Stauber RH. Survivin's Dual Role - An Export's View. Cell Cycle, 2007, 6(5):518-21.


Molekularbiologie I

ZMB/Faculty of Biology
University of Duisburg-Essen
45117 Essen

Room S05 T04 B27