ZMB Member Barbara Saccà, Publications

Prof. Dr. Barbara Saccà

Selected Publications

Kosinski R, Mukhortava A, Pfeifer W, Candelli A, Rauch P, Saccà B. Sites of high local frustration in DNA origami. Nat Commun. 2019 Mar 5;10(1):1061.

Pfeifer W, Lill P, Gatsogiannis C, Saccà B. Hierarchical Assembly of DNA Filaments with Designer Elastic Properties. ACS Nano. 2018 Jan 23;12(1):44-55.

Sprengel A, Lill P, Stegemann P, Bravo-Rodriguez K, Schöneweiß EC, Merdanovic M, Gudnason D, Aznauryan M, Gamrad L, Barcikowski S, Sanchez-Garcia E, Birkedal V, Gatsogiannis C, Ehrmann M, Saccà B. Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions. Nat Commun. 2017 Feb 16;8:14472.

Streich C, Akkari L, Decker C, Bormann J, Rehbock C, Müller-Schiffmann A, Niemeyer FC, Nagel-Steger L, Willbold D, Sacca B, Korth C, Schrader T, Barcikowski S. Characterizing the Effect of Multivalent Conjugates Composed of Aβ-Specific Ligands and Metal Nanoparticles on Neurotoxic Fibrillar Aggregation. ACS Nano. 2016 Aug 23;10(8):7582-97.

Pfeifer W, Sacca B. From Nano to Macro through Hierarchical Self-Assembly: The DNA Paradigm. Chembiochem. 2016 Jun 16;17(12):1063-80. Review.

Poepsel S, Sprengel A, Sacca B, Kaschani F, Kaiser M, Gatsogiannis C, Raunser S, Clausen T, Ehrmann M. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nat Chem Biol. Nov;11(11):862-9.

Saccà B, Ishitsuka Y, Meyer R, Sprengel A, Schöneweiß EC, Nienhaus GU, Niemeyer CM. Reversible Reconfiguration of DNA Origami Nanochambers Monitored by Single-Molecule FRET. Angew Chem Int Ed Engl. 2015 Jan 28.

Saccà B. Nucleic acids nanotechnology. Methods. 2014 May 15;67(2):103-4.

Erkelenz M, Bauer DM, Meyer R, Gatsogiannis C, Raunser S, B. Saccà, Niemeyer CM. A Facile Method for Preparation of Tailored Scaffolds for DNA-Origami. Small. 2014 Jan 15;10(1):73-7.

B. Saccà, B. Siebers, R. Meyer, M. Bayer and C.M. Niemeyer. Nanolattices of switchable DNA-based motors. Small. 2012 Oct 8;8(19):3000-8.

A. Tennstaedt, S. Pöpsel, L. Truebestein, P. Hauske, A. Brockmann, N. Schmidt, I. Irle, B. Saccà, C.M. Niemeyer, R. Brandt, H. Ksiezak-Reding, A.L. Tirniceriu, R. Egensperger, A. Baldi, L. Dehmelt, M. Kaiser, R. Huber, T. Clausen and M. Ehrmann. Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates. J. Biol. Chem. 2012, 287, 20931-20941.

B. Saccà and C.M. Niemeyer. Covalent tethering of protruding arms for addressable DNA nanostructures. Small 2011, 7, 2887-2898.

B. Saccà and C.M. Niemeyer. Functionalization of DNA nanostructures with proteins. Part of a themed issue on the advances in DNA-based nanotechnology. Chemical Society Reviews, 2011, 40, 5910-5921.

B. Saccà and C.M. Niemeyer. DNA origami: the art of folding DNA. Angew. Chem. Int. Ed. Engl. 2011, 51, 58-66.

B. Saccà, R. Meyer, M. Erkelenz, K.S. Rabe, K. Kiko, A. Arndt, and C.M. Niemeyer. Orthogonal protein decoration of DNA origami. Angew. Chem. Int. Ed. Engl. 2010, 49, 9378-9383.

B. Saccà, R. Meyer and C.M. Niemeyer. Temperature-dependent FRET spectroscopy for the high-throughput analysis of self-assembled DNA nanostructures in real time. Nat. Protoc. 2009, 4, 271-285.

B. Saccà, R. Meyer and C.M. Niemeyer. Analysis of the self-assembly of 4x4 DNA tiles by temperature-dependent FRET spectroscopy. ChemPhysChem 2009, 10, 3239-3248.

U. Feldkamp, B. Saccà and C.M. Niemeyer. Dendritic DNA building blocks for amplified detection assays and biomaterials. Angew. Chem. Int. Ed. Engl. 2009, 48, 5996-6000.

B. Saccà, R. Meyer, U. Feldkamp, H. Schroeder and C.M. Niemeyer*. High-throughput, real-time monitoring of the self-assembly of DNA nanostructures by FRET spectroscopy. Angew. Chem. Int. Ed. Engl. 2008, 47, 2135-2137.

A. De Cian, J. Gros, A. Guédin, M. Haddi, S. Lyonnais, L. Guittat, J.F. Riou, C. Trentesaux, B. Saccà, L. Lacroix, P. Alberti and J.L. Mergny. DNA and RNA quadruplex ligands. Nucleic Acids Symp Ser (Oxf). 2008, 52, 7-8.

A. De Cian, L. Guittat, M. Kaiser, B. Saccà, S. Amrane, A. Bourdoncle, P. Alberti, M.P. Teulade-Fichou, L. Lacroix and J.L. Mergny. Fluorescence-based melting assays for studying quadruplex ligands. Methods 2007, 42, 183-195.

B. Saccà, C. Conti, J. Herrick, C. Lalou, Y. Pommier and A. Bensimon. Replication fork velocities at adjacent replication origins are coordinately modified during DNA replication in human cells. Mol. Biol. Cell 2007, 18, 3059-3067.

V. A. Rao, C. Conti, Z. Miao, J. Guirouilh-Barbat, S. M. Davies, B. Saccà, I. Hickson, A. Bensimon and Y. Pommier. Endogenous -H2AX-ATM-Chk2 checkpoint activation in BLM-deficient cells is related to DNA replication arrested forks. Mol. Cancer Res. 2007, 5, 713-724.

P. Alberti, A. Bourdoncle, B. Saccà, L. Lacroix and J.L. Mergny. DNA nanomachines and nanostructures involving quadruplexes. Org. Biomol. Chem. 2006, 4, 3383-3391.

J.L. Mergny, A. De Cian, A. Ghelab, B. Saccà and L. Lacroix. Kinetics of tetramolecular quadruplexes. Nucleic Acids Res. 2005, 32, 1-14.

B. Saccà, L. Lacroix and J.L. Mergny. The effect of chemical modifications on the thermal stability of different G-quadruplex-forming oligonucleotides. Nucleic Acids Res. 2005, 33, 1182-1192.

S. Amrane, B. Saccà, M. Mills, M. Chauhan, H.H. Klump and J.L. Mergny. Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats. Nucleic Acids Res. 2005, 33, 4065-4077.

E.K. Sinner, U. Reuning, F. Nese Kök, B. Saccà, L. Moroder, W. Knoll and D. Oesterhelt. Incorporation of integrins into artificial planar lipid membranes: characterization by plasmon-enhanced fluorescence spectroscopy. Anal. Biochemistry 2004, 333, 216-224.

C. Renner, B. Saccà and L. Moroder. Synthetic heterotrimeric collagen peptides as mimics of cell adhesion sites of the basement membranes. Biopolymers 2004, 76, 34-47.

B. Saccà, S. Fiori and L. Moroder. Studies of the local conformational properties of the cell-adhesion domain of collagen type IV in synthetic heterotrimeric peptides. Biochemistry 2003, 42, 3429-3436.

B. Saccà and L. Moroder. Synthesis of heterotrimeric collagen peptides containing the 11 integrin recognition site of collagen type IV. J. Pept. Sci. 2002, 8, 192-204.

B. Saccà, D. Barth and L. Moroder. Conformation-dependent side reactions in interstrand-disulfide bridging of trimeric collagenous peptides by regioselective cysteine chemistry. J. Pept. Sci. 2002, 8, 205-210.

S. Fiori, B. Saccà and L. Moroder. Structural properties of a collagenous heterotrimer that mimics the collagenase cleavage site of collagen type I. J. Mol. Biol. 2002, 319, 1235-1242.

B. Saccà, E.K. Sinner, J. Kaiser, C. Lübken, J. Eble and L. Moroder. Binding and docking of synthetic heterotrimeric collagen type IV peptides with alpha1beta1 integrin. Chembiochem 2002, 3, 904-907.

B. Saccà, C. Renner and L. Moroder. The chain register in heterotrimeric collagen peptides affects triple helix stability and folding kinetics. J. Mol. Biol. 2002, 324, 309-318.

Research Group


ZMB/Faculty of Biology
University of Duisburg-Essen
45117 Essen

Room T03 R01 D63

Recent Paper
Nature Chemical Biology

Determinants of amyloid fibril degradation by the PDZ protease HTRA1

Simon Poepsel, Andreas Sprengel, Barbara Sacca, Farnusch Kaschani, Markus Kaiser, Christos Gatsogiannis, Stefan Raunser, Tim Clausen & Michael Ehrmann