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X-PEEM locates protein adsorbed on surface of a phase segregated polymer
by Cynthia Morin, Adam Hitchcock, Andrew Doran and Andreas Scholl

Map of human serum fibrinogen (Fg) on a polystyrene (PS), polymethylmethacrylate (PMMA) blend derived from a C 1s X-PEEM image sequence. The fibrinogen (blue) has a strong preference for adsorption on the PS domains (red). The yellow in the PMMA domains (green) arises from PS microdomains (R+G=Y - see color circles) and some radiation damage.
Details: The fibrinogen was adsorbed from a 0.05 mg/ml pH=7 phosphate buffer solution onto a phase segregated polymer blend formed by spin coating a 30% polystyrene (PS), 70% polymethylmethacrylate (PMMA) blend from dilute toluene solution (1 wt% polymer). A C 1s image sequence (49 energies, 280-320 eV) was recorded using the photoemission electron microscope (PEEM-2) at the ALS and analyzed to generate component maps which were then combined into this color composite map.


Cynthia Morin1, Adam Hitchcock1, Andrew Doran2, Andreas Scholl2

Affiliation: (1) McMaster University; (2) Advanced Light Source
PEEM-2 at BL 7.3.1 at the Advanced Light Source
Website: http://unicorn.mcmaster.ca/highlights/fg-pspmma-peem/fg-pspmma-peem.html
Publication: C. Morin et al. J. Electron Spectrosc. 137-140 (2004) 785-794


University of Duisburg-Essen | AG Horn-von Hoegen


Au (5x3.2) Reconstruction Domains grown on Si(111). Courtesy of F. Meyer zu Heringdorf and R. Tromp, IBM.

Growth of Lead on Cu(111). Courtesy of Gary Kellog, Sandia

PEEM Images of Pentacene Islands on Si. Courtesy of F. Meyer zu Heringdorf and R. Tromp, IBM