DeltaVision Elite high resolution microscope
Widefield Systems DeltaVision Elite high resolution microscope
Room: S05 R01 H24
Main Applications
- widefield deconvolution fluorescence microscope
- transmitted light applications (brightfield, DIC)
- multi-dimensional live cell + fixed specimen imaging
- multi-position acquisition
- Low light imaging of sensitive samples
Funding
DeltaVision Elite widefield deconvolution microscope was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 361068552.
Technical Specifications
Microscope and Accessories
- Inverted Olympus IX-71 microscope stand
- UltimateFocus Modul (hardware autofocus system)
- Automated high precision Flexure stage (long travel, 25x50 mm), “3D multisite visiting”
- Environmental chamber with temperature & CO2 control for live cell imaging
Camera
- pco.edge 5.5 sCMOS camera, 2560 x 2160 pixels, 6.5 x 6.5 µm pixel size, 16.6 x 14 mm sensor format, 16-bit digitization, 200 fps at 1024 x 1024
Light sources
- DV Light SSI 7 color ultra-fast solid state illumination
excitation filters
| Fluorochrome | excitation bandpass [nm] |
| DAPI | 390/18 |
| CFP | 438/24 |
| GFP/TRITC | 475/28 |
| YFP | 513/17 |
| TRITC | 542/27 |
| mCherry/Alexa594 | 575/25 |
| Cy5 | 632/22 |
- LED transmitted light module
Emission filters & Polychroic beamsplitters
Single band emission filters
| Fluorochrome | emission bandpass [nm] |
| DAPI | 435/48 (use with Polychroic #1 or #2) |
| CFP | 475/24 (use with Polychroic #3) |
| GFP/TRITC | 525/48 (use with Polychroic #1 or #2) |
| YFP | 548/22 (use with Polychroic #3) |
| TRITC | 597/45 (use with Polychroic # 1) |
| mCherry/Alexa594 | 625/45 (use with Polychroic #2 or #3) |
| Cy5 | 679/37 (use with Polychroic #1 or #2) |
Polychroic beamsplitters
| Position | Fluorochromes |
| Polychroic #1 | DAPI/FITC/TRITC/Cy5 |
| Polychroic #2 | DAPI/FITC/mCherry/Alexa594/Cy5 |
| Polychroic #3 | CFP/YFP/mCherry |
Objectives
| name |
magnification |
NA | immersion |
| Super-Plan Apo 20x/0.75 DIC | 20x | 0.75 | dry |
| Plan Apo 60x/1.42 oil DIC | 60x | 1.42 | oil |
| Super-Plan Apo 100x/1.4 oil DIC | 100x | 1.4 | oil |
Software
SoftWoRx 6 and Resolve 3D (integrated in SoftWoRx) with software modules for Deconvolution (image restoration), Cell tracking, Point visiting, Optic axial integration (OAI) and 3D visualization