30.11.2024 - 10:42:32

How HTRA1 Degrades Folded Proteins – A Collaborative Discovery

The Ehrmann group (A06), in collaboration with the Kaiser (A04) and Hellerschmied (Z02) groups, the ACCE Facility, and other partners, has revealed how the human protease HTRA1 degrades not only misfolded but also folded proteins.
Using mass spectrometry, CD spectroscopy, and bioinformatics, the team identified 178 cleavage sites in ANXA1, uncovering how HTRA1 recognizes specific sequences and unfolds its substrates during degradation. The approach also offers a blueprint for studying post-translational modifications in complex protein systems