Dr. Christine Beuck

Contact

  • +49 (0)201 183-2929
  • E-mail
  • S03 S00 A35

 

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Research

Nuclear Magnetic Resonance (NMR) spectroscopy

NMR spectroscopy is a versatile tool to study proteins and their molecular interactions. As part of the CRC 1093, I investigate both the interactions of proteins with their natural binding partners and synthetic ligands designed to target protein surfaces. NMR is a powerful method that can directly determine the protein residues involved in interactions with other biomolecules or synthetic ligands. I established the use of side chain specific NMR experiments for lysine and arginine residues to precisely monitor supramolecular ligand binding, which, in contrast to the standard amide 15N-HSQC experiment, allows to rank multiple binding sites within one protein. For larger proteins, fast-acquisition (BEST-TROSY; SOFAST) as well as Methyl-TROSY experiments are employed. Furthermore, NMR is used to determine the structures of proteins or isolated domains and probe their size, oligomerization status and dynamics or reaction kinetics.

Protein Structure Determination

I elucidate the structures of proteins with both solution NMR spectroscopy and X-Ray crystallography.

NMR structures:

X-Ray structures:

Biochemical Characterization of Protein Complexes

To perform their task within the cell, proteins are interacting with a multitude of other biomolecules like other proteins, nu

Curriculum Vitae

Professional career

Since 2014 Assistant Professor (Habilitandin) with Prof. Dr. P. Bayer, University of Duisburg-Essen, Germany
2014 Staff Scientist with Prof. Dr. J. R. Williamson, The Scripps Research Institute, La Jolla, USA
2012 - 2014 Senior Research Associate with Prof. Dr. J. R. Williamson, The Scripps Research Institute, La Jolla, USA
2005 - 2012 Research Associate (Postdoc) with Prof. Dr. J. R. Williamson, The Scripps Research Institute, La Jolla, USA
2005 Guest scientist with Prof. Dr. M. Shionoya, Tokyo University, Tokyo, Japan, as part of the GDCh/DFG Japan exchange program 2005
2003 Exchange researcher with Dr. habil. S. Klimašauskas, Institute of Biotechnology, Vilnius, Lithuania

 

Scientific education and degrees

2001 - 2005 Ph.D. in Chemistry, with Prof. Dr. E. Weinhold, RWTH Aachen
1996 - 2001 Study of Chemistry (Dipl.) at the Technical University of Dortmund, Diploma thesis with Prof. Dr. E. Weinhold, Max Planck Institute for Molecular Physiology

 

Publications

    Journal articles

  • Hommel, Katrin; Theisen, Sebastian; Blueggel, Mike; Niemeyer, Felix C.; Beuck, Christine; Bayer, Peter; Zadmard, Reza; Schrader, Thomas; Knauer, Shirley K.
    Highly Preorganized Calixarene Tweezers for Protein Recognition.
    In: Biomacromolecules, Vol. 26, 2025, Nr. 8, pp. 5310 – 5319
    DOI
  • Kirupakaran, Abbna; van den Boom, Johannes; Blueggel, Mike; Beuck, Christine; Niemeyer, Felix; Hayduk, Matthias; Balszuweit, Jan; Bayer, Peter; Voskuhl, Jens; Meyer, Hemmo; Schrader, Thomas
    Molecular Tweezers Block the Functional Pore of a Protein Machine
    In: Journal of the American Chemical Society: JACS, Vol. 147, 2025, Nr. 20, pp. 16836 – 16849
    DOI (Open Access)
  • Bietti, Antonio; Kauth, Alisa-Maite; Hommel, Katrin; Blüggel, Mike; Mohr, Laurenz; Niemeyer, Felix; Beuck, Christine; Bayer, Peter; Knauer, Shirley; Ravoo, Bart Jan; Schrader, Thomas
    Molecular Tweezers with Built-in Photoswitches Modulate Protein Activity
    In: RSC Chemical Biology, 2025, in press
    DOI (Open Access)
  • Bietti, Antonio L. Figueroa; Kauth, Alisa-Maite A.; Hommel, Katrin; Blueggel, Mike; Mohr, Laurenz; Niemeyer, Felix C.; Beuck, Christine; Bayer, Peter; Knauer, Shirley K.; Jan Ravoo, Bart; Schrader, Thomas
    Photoresponsive molecular tweezers modulate Taspase 1 activity
    In: RSC Chemical Biology, 2025, in press
    DOI (Open Access)
  • Kost, Niklas; Prymak, Oleg; Loza, Kateryna; Beuck, Christine; Bayer, Peter; Weidenthaler, Claudia; Heggen, Marc; Oliveira, Cristiano L. P.; Epple, Matthias
    Synthesis and characterization of dodecylamine-capped ultrasmall metallic palladium nanoparticles (2 nm)
    In: Nanoscale Advances, 2025, in press
    DOI (Open Access)
  • Kost, Niklas; Prymak, Oleg; Loza, Kateryna; Heggen, Marc; Oliveira, Cristiano L. P.; Beuck, Christine; Bayer, Peter; Epple, Matthias
    Synthesis and characterization of ultrasmall rhenium nanoparticles (1.5 nm)
    In: Journal of Nanoparticle Research, Vol. 27, 2025, Nr. 8, 218
    DOI (Open Access)
  • Wagner, Lisa-Sofie; Huber, Alexander; Loza, Kateryna; Beuck, Christine; Heggen, Marc; Bayer, Peter; Voskuhl, Jens; Epple, Matthias
    Temperature‐Resolved Fluorescence Quenching on Ultrasmall Gold Nanoparticles Conjugated with Dye‐Labeled Peptides
    In: Nano Select, 2025, in press
    DOI (Open Access)
  • Sternberg, Anna; Borger, Jennifer Lynne; Thies, Mathilda; Matena, Anja; Blüggel, Mike; Kamba, Bianca; Beuck, Christine; Kaschani, Farnusch; Kaiser, Markus; Bayer, Peter
    The Actin‐Binding Prolyl‐Isomerase Par17 Sustains Its Substrate Selectivity by Interdomain Allostery
    In: Proteins: Structure, Function and Genetics, Vol. 93, 2025, Nr. 9, pp. 1481 – 1497
    DOI (Open Access)
  • Wagner, Lisa-Sofie; Thiele, Tobias; Loza, Kateryna; Beuck, Christine; Bayer, Peter; Heggen, Marc; Giese, Michael R. A.; Epple, Matthias
    Ultrasmall Gold Nanoparticles (2 nm) Decorated with a High Density of Photochemically Switchable Ligands
    In: Chemistry - A European Journal, 2025
    DOI (Open Access)
  • Hommel, Katrin; Kauth, Alisa-Maite A.; Kirupakaran, Abbna; Theisen, Sebastian; Hayduk, Matthias; Niemeyer, Felix C.; Beuck, Christine; Zadmard, Reza; Bayer, Peter; Jan Ravoo, Bart; Voskuhl, Jens; Schrader, Thomas; Knauer, Shirley K.
    Functional Linkers Support Targeting of Multivalent Tweezers to Taspase1
    In: Chemistry - A European Journal, Vol. 30, 2024, Nr. 56, e202401542
    DOI (Open Access)
  • Schillinger, Jasmin; Koci, Michelle; Bravo-Rodriguez, Kenny; Heilmann, Geronimo; Kaschani, Farnusch; Kaiser, Markus; Beuck, Christine; Luecke, Hartmut; Huber, Robert; Hellerschmied-Jelinek, Doris; Burston, Steven G.; Ehrmann, Michael
    High resolution analysis of proteolytic substrate processing
    In: The Journal of Biological Chemistry (JBC), Vol. 300, 2024, Nr. 11, 107812
    DOI, Online Full Text (Open Access)
  • Wolff, Natalie; Białas, Nataniel; Loza, Kateryna; Heggen, Marc; Schaller, Torsten; Niemeyer, Felix; Weidenthaler, Claudia; Beuck, Christine; Bayer, Peter; Prymak, Oleg; Oliveira, Cristiano L. P.; Epple, Matthias
    Increased Cytotoxicity of Bimetallic Ultrasmall Silver–Platinum Nanoparticles (2 nm) on Cells and Bacteria in Comparison to Silver Nanoparticles of the Same Size
    In: Materials, Vol. 17, 2024, Nr. 15, 3702
    DOI (Open Access)
  • Wolff, Natalie; Beuck, Christine; Schaller, Torsten; Epple, Matthias
    Possibilities and limitations of solution-state NMR spectroscopy to analyze the ligand shell of ultrasmall metal nanoparticles
    In: Nanoscale Advances, Vol. 6, 2024, Nr. 13, pp. 3285 – 3298
    DOI (Open Access)
  • Beaufort, Nathalie; Ingendahl, Linda; Merdanovic, Melisa; Schmidt, Andree; Podlesainski, David; Richter, Tim; Neumann, Thorben; Kuszner, Michael; Vetter, Ingrid R.; Stege, Patricia; Burston, Steven G.; Filipovic, Anto; Ruiz-Blanco, Yasser B.; Bravo Rodriguez, Kenny; Mieres-Perez, Joel; Beuck, Christine; Uebel, Stephan; Zobawa, Monika; Schillinger, Jasmin; Malik, Rainer; Todorov-Völgyi, Katalin; Rey, Juliana; Roberti, Annabell; Hagemeier, Birte; Wefers, Benedikt; Müller, Stephan A.; Wurst, Wolfgang; Sanchez-Garcia, Elsa; Zimmermann, Alexander; Hu, Xiao-Yu; Clausen, Tim; Huber, Robert; Lichtenthaler, Stefan F.; Schmuck, Carsten; Giese, Michael; Kaiser, Markus; Ehrmann, Michael; Dichgans, Martin
    Rational correction of pathogenic conformational defects in HTRA1
    In: Nature Communications, Vol. 15, 2024, Nr. 1, 5944
    DOI (Open Access)
  • Wagner, Lisa-Sofie; Prymak, Oleg; Schaller, Torsten; Beuck, Christine; Loza, Kateryna; Niemeyer, Felix; Gumbiowski, Nina; Kostka, Kathrin; Bayer, Peter; Heggen, Marc; Oliveira, Cristiano L. P.; Epple, Matthias
    The Molecular Footprint of Peptides on the Surface of Ultrasmall Gold Nanoparticles (2 nm) Is Governed by Steric Demand
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry, Vol. 128, 2024, Nr. 17, pp. 4266 – 4281
    DOI
  • Neblik, Jonas; Kirupakaran, Abbna; Beuck, Christine; Mieres-Perez, Joel; Niemeyer, Felix; Le, My-Hue; Telgheder, Ursula; Schmuck, Jessica Felice; Dudziak, Alexander; Bayer, Peter; Sanchez-Garcia, Elsa; Westermann, Stefan; Schrader, Thomas
    Multivalent Molecular Tweezers Disrupt the Essential NDC80 Interaction with Microtubules
    In: Journal of the American Chemical Society: JACS, Vol. 145, 2023, Nr. 28, pp. 15251 – 15264
    DOI
  • Seiler, Theresa; Lennartz, Annika; Klein, Kai; Hommel, Katrin; Figueroa Bietti, Antonio; Hadrovic, Inesa; Kollenda, Sebastian; Sager, Jonas; Beuck, Christine; Chlosta, Emilia; Bayer, Peter; Juul-Madsen, Kristian; Vorup-Jensen, Thomas; Schrader, Thomas; Epple, Matthias; Knauer, Shirley; Hartmann, Laura
    Potentiating Tweezer Affinity to a Protein Interface with Sequence-Defined Macromolecules on Nanoparticles
    In: Biomacromolecules, Vol. 24, 2023, Nr. 8, pp. 3666 – 3679
    DOI
  • Blüggel, Mike; Kröning, Alexander; Kracht, Matthias; van den Boom, Johannes; Dabisch, Matthias; Göhring, Anna; Kaschani, Farnusch; Kaiser, Markus; Bayer, Peter; Meyer, Hemmo; Beuck, Christine
    The UBX domain in UBXD1 organizes ubiquitin binding at the C-terminus of the VCP/p97 AAA-ATPase
    In: Nature Communications, Vol. 14, 2023, Nr. 1, 3258
    DOI, Online Full Text (Open Access)
  • Wolff, Natalie; Loza, Kateryna; Heggen, Marc; Schaller, Torsten; Niemeyer, Felix; Bayer, Peter; Beuck, Christine; Oliveira, Cristiano L P; Prymak, Oleg; Weidenthaler, Claudia; Epple, Matthias
    Ultrastructure and Surface Composition of Glutathione-Terminated Ultrasmall Silver, Gold, Platinum, and Alloyed Silver-Platinum Nanoparticles (2 nm)
    In: Inorganic Chemistry, Vol. 62, 2023, Nr. 42, pp. 17470 – 17485
    DOI
  • Höing, Alexander; Struth, Robin; Beuck, Christine; Rafieiolhosseini, Neda; Hoffmann, Daniel; Stauber, Roland H.; Bayer, Peter; Niemeyer, Jochen; Knauer, Shirley
    Dual activity inhibition of threonine aspartase 1 by a single bisphosphate ligand
    In: RSC Advances, Vol. 12, 2022, Nr. 53, pp. 34176 – 34184
    DOI (Open Access)
  • Höing, Alexander; Kirupakaran, Abbna; Beuck, Christine; Pörschke, Marius; Niemeyer, Felix; Seiler, Theresa; Hartmann, Laura; Bayer, Peter; Schrader, Thomas; Knauer, Shirley
    Recognition of a Flexible Protein Loop in Taspase 1 by Multivalent Supramolecular Tweezers
    In: Biomacromolecules, Vol. 23, 2022, Nr. 11, pp. 4504 – 4518
    DOI
  • Wetzel, Oliver; Prymak, Oleg; Loza, Kateryna; Gumbiowski, Nina; Heggen, Marc; Bayer, Peter; Beuck, Christine; Weidenthaler, Claudia; Epple, Matthias
    Water-Based Synthesis of Ultrasmall Nanoparticles of Platinum Group Metal Oxides (1.8 nm)
    In: Inorganic Chemistry, Vol. 61, 2022, Nr. 12, pp. 5133 – 5147
    DOI
  • Wetzel, Oliver; Hosseini, Shabnam; Loza, Kateryna; Heggen, Marc; Prymak, Oleg; Bayer, Peter; Beuck, Christine; Schaller, Torsten; Niemeyer, Felix; Weidenthaler, Claudia; Epple, Matthias
    Metal-Ligand Interface and Internal Structure of Ultrasmall Silver Nanoparticles (2 nm)
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry, Vol. 125, 2021, Nr. 21, pp. 5645 – 5659
    DOI
  • van der Meer, Selina Beatrice; Hadrovic, Inesa; Meiners, Annika; Loza, Kateryna; Heggen, Marc; Knauer, Shirley; Bayer, Peter; Schrader, Thomas; Beuck, Christine; Epple, Matthias
    New tools to probe the protein surface : Ultrasmall gold nanoparticles carry amino acid binders
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry, Vol. 125, 2021, Nr. 1, pp. 115 – 127
    DOI (Open Access)
  • Ruks, Tatjana; Loza, Kateryna; Heggen, Marc; Prymak, Oleg; Sehnem, Andre Luiz; Oliveira, Cristiano L. P.; Bayer, Peter; Beuck, Christine; Epple, Matthias
    Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting
    In: ACS Applied Bio Materials, Vol. 4, 2021, Nr. 1, pp. 945 – 965
    DOI (Open Access)
  • Meiners, Annika; Bäcker, Sandra; Hadrovic, Inesa; Heid, Christian; Beuck, Christine; Ruiz-Blanco, Yasser B.; Mieres-Perez, Joel; Pörschke, Marius; Grad, Jean-Noël; Vallet, Cecilia; Hoffmann, Daniel; Bayer, Peter; Sánchez-García, Elsa; Schrader, Thomas; Knauer, Shirley
    Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
    In: Nature Communications, Vol. 12, 2021, Nr. 1, pp. 1505
    DOI, Online Full Text (Open Access)
  • Ruks, Tatjana; Loza, Kateryna; Heggen, Marc; Ottmann, Christian; Bayer, Peter; Beuck, Christine; Epple, Matthias
    Targeting the Surface of the Protein 14-3-3 by Ultrasmall (1.5 nm) Gold Nanoparticles Carrying the Specific Peptide CRaf
    In: ChemBioChem, Vol. 22, 2021, Nr. 8, pp. 1456 – 1463
    DOI (Open Access)
  • Vallet, Cecilia; Aschmann, Dennis; Beuck, Christine; Killa, Matthias; Meiners, Annika; Mertel, Marcel; Ehlers, Martin; Bayer, Peter; Schmuck, Carsten; Giese, Michael R. A.; Knauer, Shirley
    Functional Disruption of the Cancer-Relevant Interaction between Survivin and Histone H3 with a Guanidiniocarbonyl Pyrrole Ligand
    In: Angewandte Chemie International Edition, Vol. 59, 2020, Nr. 14, pp. 5567 – 5571
    DOI (Open Access)
  • Bayer, Peter; Matena, Anja; Beuck, Christine
    NMR Spectroscopy of supramolecular chemistry on protein surfaces
    In: Beilstein Journal of Organic Chemistry, Vol. 16, 2020, pp. 2505 – 2522
    DOI (Open Access)
  • van der Meer, Selina; Loza, Kateryna; Wey, Karolin; Heggen, Marc; Beuck, Christine; Bayer, Peter; Epple, Matthias
    Click chemistry on the surface of ultrasmall gold nanoparticles (2 nm) for covalent ligand attachment followed by nmr spectroscopy
    In: Langmuir, Vol. 35, 2019, Nr. 22, pp. 7191 – 7204
    DOI
  • Bartsch, Lina; Bartel, Maria; Gigante, Alba; Iglesias-Fernández, Javier; Ruiz-Blanco, Yasser B.; Beuck, Christine; Briels, Jeroen; Tötsch, Niklas; Bayer, Peter; Sánchez-García, Elsa; Ottmann, Christian; Schmuck, Carsten
    Multivalent Ligands with Tailor-Made Anion Binding Motif as Stabilizers of Protein–Protein Interactions
    In: ChemBioChem, Vol. 20, 2019, Nr. 23, pp. 2921 – 2926
    DOI
  • Ruks, Tatjana; Beuck, Christine; Schaller, Torsten; Niemeyer, Felix; Zähres, Manfred; Loza, Kateryna; Heggen, Marc; Hagemann, Ulrich; Mayer, Christian; Bayer, Peter; Epple, Matthias
    Solution NMR Spectroscopy with Isotope-Labeled Cysteine (¹³C and ¹⁵N) Reveals the Surface Structure of l -Cysteine-Coated Ultrasmall Gold Nanoparticles (1.8 nm)
    In: Langmuir, Vol. 35, 2019, Nr. 3, pp. 767 – 778
    DOI
  • Blüggel, Mike; van den Boom, Johannes; Meyer, Hemmo; Bayer, Peter; Beuck, Christine
    Structure of the PUB Domain from Ubiquitin Regulatory X Domain Protein 1 (UBXD1) and Its Interaction with the p97 AAA+ ATPase
    In: Biomolecules, Vol. 9, 2019, Nr. 12, 876
    DOI, Online Full Text (Open Access)
  • Hogeweg, Anna; Sowislok, Andrea; Schrader, Thomas; Beuck, Christine
    An NMR Method To Pinpoint Supramolecular Ligand Binding to Basic Residues on Proteins
    In: Angewandte Chemie International Edition, Vol. 56, 2017, Nr. 46, pp. 14758 – 14762
    DOI
  • Trusch, Franziska; Kowski, Klaus; Bravo-Rodriguez, Kenny; Beuck, Christine; Sowislok, Andrea; Wettig, Burkhard; Matena, Anja; Sánchez-García, Elsa; Meyer, Hemmo; Schrader, Thomas; Bayer, Peter
    Molecular tweezers target a protein-protein interface and thereby modulate complex formation
    In: Chemical Communications: ChemComm, Vol. 52, 2016, Nr. 98, pp. 14141 – 14144
    DOI (Open Access)
  • van den Boom, Johannes; Trusch, Franziska; Hoppstock, Lukas; Beuck, Christine; Bayer, Peter
    Structural Characterization of the Loop at the Alpha-Subunit C-Terminus of the Mixed Lineage Leukemia Protein Activating Protease Taspase1
    In: PLoS ONE, Vol. 11, 2016, Nr. 3, pp. e0151431
    DOI, Online Full Text (Open Access)
  • Beuck, Christine; Williamson, James R.; Wüthrich, Kurt; Serrano, Pedro
    The acidic domain is a unique structural feature of the splicing factor SYNCRIP
    In: Protein Science, Vol. 25, 2016, Nr. 8, pp. 1545 – 1550
    DOI (Open Access)
  • Beuck, Christine; Weinhold, Elmar
    Reversibly locked thionucleobase pairs in DNA to study base flipping enzymes
    In: Beilstein Journal of Organic Chemistry, Vol. 10, 2014, pp. 2293 – 2306
    DOI (Open Access)