Dr. Christine Beuck

Contact

  • +49 (0)201 183-2929
  • E-mail
  • S03 S00 A35

 

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Research

Nuclear Magnetic Resonance (NMR) spectroscopy

NMR spectroscopy is a versatile tool to study proteins and their molecular interactions. As part of the CRC 1093, I investigate both the interactions of proteins with their natural binding partners and synthetic ligands designed to target protein surfaces. NMR is a powerful method that can directly determine the protein residues involved in interactions with other biomolecules or synthetic ligands. I established the use of side chain specific NMR experiments for lysine and arginine residues to precisely monitor supramolecular ligand binding, which, in contrast to the standard amide 15N-HSQC experiment, allows to rank multiple binding sites within one protein. For larger proteins, fast-acquisition (BEST-TROSY; SOFAST) as well as Methyl-TROSY experiments are employed. Furthermore, NMR is used to determine the structures of proteins or isolated domains and probe their size, oligomerization status and dynamics or reaction kinetics.

Protein Structure Determination

I elucidate the structures of proteins with both solution NMR spectroscopy and X-Ray crystallography.

NMR structures:

X-Ray structures:

Biochemical Characterization of Protein Complexes

To perform their task within the cell, proteins are interacting with a multitude of other biomolecules like other proteins, nu

Curriculum Vitae

Professional career

Since 2014 Assistant Professor (Habilitandin) with Prof. Dr. P. Bayer, University of Duisburg-Essen, Germany
2014 Staff Scientist with Prof. Dr. J. R. Williamson, The Scripps Research Institute, La Jolla, USA
2012 - 2014 Senior Research Associate with Prof. Dr. J. R. Williamson, The Scripps Research Institute, La Jolla, USA
2005 - 2012 Research Associate (Postdoc) with Prof. Dr. J. R. Williamson, The Scripps Research Institute, La Jolla, USA
2005 Guest scientist with Prof. Dr. M. Shionoya, Tokyo University, Tokyo, Japan, as part of the GDCh/DFG Japan exchange program 2005
2003 Exchange researcher with Dr. habil. S. Klimašauskas, Institute of Biotechnology, Vilnius, Lithuania

 

Scientific education and degrees

2001 - 2005 Ph.D. in Chemistry, with Prof. Dr. E. Weinhold, RWTH Aachen
1996 - 2001 Study of Chemistry (Dipl.) at the Technical University of Dortmund, Diploma thesis with Prof. Dr. E. Weinhold, Max Planck Institute for Molecular Physiology

 

Publications

    Journal articles

  • Neblik, Jonas; Kirupakaran, Abbna; Beuck, Christine; Mieres-Perez, Joel; Niemeyer, Felix; Le, My-Hue; Telgheder, Ursula; Schmuck, Jessica Felice; Dudziak, Alexander; Bayer, Peter; Sanchez-Garcia, Elsa; Westermann, Stefan; Schrader, Thomas
    Multivalent Molecular Tweezers Disrupt the Essential NDC80 Interaction with Microtubules
    In: Journal of the American Chemical Society: JACS Vol. 145 (2023) Nr. 28, pp. 15251 - 15264
    Online Full Text: dx.doi.org/
  • Seiler, Theresa; Lennartz, Annika; Klein, Kai; Hommel, Katrin; Figueroa Bietti, Antonio; Hadrovic, Inesa; Kollenda, Sebastian; Sager, Jonas; Beuck, Christine; Chlosta, Emilia; Bayer, Peter; Juul-Madsen, Kristian; Vorup-Jensen, Thomas; Schrader, Thomas; Epple, Matthias; Knauer, Shirley; Hartmann, Laura
    Potentiating Tweezer Affinity to a Protein Interface with Sequence-Defined Macromolecules on Nanoparticles
    In: Biomacromolecules Vol. 24 (2023) Nr. 8, pp. 3666 - 3679
    Online Full Text: dx.doi.org/
  • Blüggel, Mike; Kröning, Alexander; Kracht, Matthias; van den Boom, Johannes; Dabisch, Matthias; Göhring, Anna; Kaschani, Farnusch; Kaiser, Markus; Bayer, Peter; Meyer, Hemmo; Beuck, Christine
    The UBX domain in UBXD1 organizes ubiquitin binding at the C-terminus of the VCP/p97 AAA-ATPase
    In: Nature Communications Vol. 14 (2023) Nr. 1, 3258
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Wolff, Natalie; Loza, Kateryna; Heggen, Marc; Schaller, Torsten; Niemeyer, Felix; Bayer, Peter; Beuck, Christine; Oliveira, Cristiano L P; Prymak, Oleg; Weidenthaler, Claudia; Epple, Matthias
    Ultrastructure and Surface Composition of Glutathione-Terminated Ultrasmall Silver, Gold, Platinum, and Alloyed Silver-Platinum Nanoparticles (2 nm)
    In: Inorganic Chemistry Vol. 62 (2023) Nr. 42, pp. 17470 - 17485
    Online Full Text: dx.doi.org/
  • Höing, Alexander; Struth, Robin; Beuck, Christine; Rafieiolhosseini, Neda; Hoffmann, Daniel; Stauber, Roland H.; Bayer, Peter; Niemeyer, Jochen; Knauer, Shirley
    Dual activity inhibition of threonine aspartase 1 by a single bisphosphate ligand
    In: RSC Advances Vol. 12 (2022) Nr. 53, pp. 34176 - 34184
    Online Full Text: dx.doi.org/ (Open Access)
  • Höing, Alexander; Kirupakaran, Abbna; Beuck, Christine; Pörschke, Marius; Niemeyer, Felix; Seiler, Theresa; Hartmann, Laura; Bayer, Peter; Schrader, Thomas; Knauer, Shirley
    Recognition of a Flexible Protein Loop in Taspase 1 by Multivalent Supramolecular Tweezers
    In: Biomacromolecules Vol. 23 (2022) Nr. 11, pp. 4504 - 4518
    Online Full Text: dx.doi.org/
  • Wetzel, Oliver; Prymak, Oleg; Loza, Kateryna; Gumbiowski, Nina; Heggen, Marc; Bayer, Peter; Beuck, Christine; Weidenthaler, Claudia; Epple, Matthias
    Water-Based Synthesis of Ultrasmall Nanoparticles of Platinum Group Metal Oxides (1.8 nm)
    In: Inorganic Chemistry Vol. 61 (2022) Nr. 12, pp. 5133 - 5147
    Online Full Text: dx.doi.org/
  • Wetzel, Oliver; Hosseini, Shabnam; Loza, Kateryna; Heggen, Marc; Prymak, Oleg; Bayer, Peter; Beuck, Christine; Schaller, Torsten; Niemeyer, Felix; Weidenthaler, Claudia; Epple, Matthias
    Metal-Ligand Interface and Internal Structure of Ultrasmall Silver Nanoparticles (2 nm)
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry Vol. 125 (2021) Nr. 21, pp. 5645 - 5659
    Online Full Text: dx.doi.org/
  • van der Meer, Selina Beatrice; Hadrovic, Inesa; Meiners, Annika; Loza, Kateryna; Heggen, Marc; Knauer, Shirley; Bayer, Peter; Schrader, Thomas; Beuck, Christine; Epple, Matthias
    New tools to probe the protein surface : Ultrasmall gold nanoparticles carry amino acid binders
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry Vol. 125 (2021) Nr. 1, pp. 115 - 127
    Online Full Text: dx.doi.org/ (Open Access)
  • Ruks, Tatjana; Loza, Kateryna; Heggen, Marc; Prymak, Oleg; Sehnem, Andre Luiz; Oliveira, Cristiano L. P.; Bayer, Peter; Beuck, Christine; Epple, Matthias
    Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting
    In: ACS Applied Bio Materials Vol. 4 (2021) Nr. 1, pp. 945 - 965
    Online Full Text: dx.doi.org/ (Open Access)
  • Meiners, Annika; Bäcker, Sandra; Hadrovic, Inesa; Heid, Christian; Beuck, Christine; Ruiz-Blanco, Yasser B.; Mieres-Perez, Joel; Pörschke, Marius; Grad, Jean-Noël; Vallet, Cecilia; Hoffmann, Daniel; Bayer, Peter; Sánchez-García, Elsa; Schrader, Thomas; Knauer, Shirley
    Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
    In: Nature Communications Vol. 12 (2021) Nr. 1, pp. 1505
    Online Full Text: dx.doi.org/ (Open Access)
  • Ruks, Tatjana; Loza, Kateryna; Heggen, Marc; Ottmann, Christian; Bayer, Peter; Beuck, Christine; Epple, Matthias
    Targeting the Surface of the Protein 14-3-3 by Ultrasmall (1.5 nm) Gold Nanoparticles Carrying the Specific Peptide CRaf
    In: ChemBioChem Vol. 22 (2021) Nr. 8, pp. 1456 - 1463
    Online Full Text: dx.doi.org/ (Open Access)
  • Vallet, Cecilia; Aschmann, Dennis; Beuck, Christine; Killa, Matthias; Meiners, Annika; Mertel, Marcel; Ehlers, Martin; Bayer, Peter; Schmuck, Carsten; Giese, Michael R. A.; Knauer, Shirley
    Functional Disruption of the Cancer-Relevant Interaction between Survivin and Histone H3 with a Guanidiniocarbonyl Pyrrole Ligand
    In: Angewandte Chemie International Edition Vol. 59 (2020) Nr. 14, pp. 5567 - 5571
    Online Full Text: dx.doi.org/ (Open Access)
  • Bayer, Peter; Matena, Anja; Beuck, Christine
    NMR Spectroscopy of supramolecular chemistry on protein surfaces
    In: Beilstein Journal of Organic Chemistry Vol. 16 (2020) pp. 2505 - 2522
    Online Full Text: dx.doi.org/ (Open Access)
  • van der Meer, Selina; Loza, Kateryna; Wey, Karolin; Heggen, Marc; Beuck, Christine; Bayer, Peter; Epple, Matthias
    Click chemistry on the surface of ultrasmall gold nanoparticles (2 nm) for covalent ligand attachment followed by nmr spectroscopy
    In: Langmuir Vol. 35 (2019) Nr. 22, pp. 7191 - 7204
    Online Full Text: dx.doi.org/
  • Bartsch, Lina; Bartel, Maria; Gigante, Alba; Iglesias-Fernández, Javier; Ruiz-Blanco, Yasser B.; Beuck, Christine; Briels, Jeroen; Tötsch, Niklas; Bayer, Peter; Sánchez-García, Elsa; Ottmann, Christian; Schmuck, Carsten
    Multivalent Ligands with Tailor-Made Anion Binding Motif as Stabilizers of Protein–Protein Interactions
    In: ChemBioChem Vol. 20 (2019) Nr. 23, pp. 2921 - 2926
    Online Full Text: dx.doi.org/
  • Ruks, Tatjana; Beuck, Christine; Schaller, Torsten; Niemeyer, Felix; Zähres, Manfred; Loza, Kateryna; Heggen, Marc; Hagemann, Ulrich; Mayer, Christian; Bayer, Peter; Epple, Matthias
    Solution NMR Spectroscopy with Isotope-Labeled Cysteine (¹³C and ¹⁵N) Reveals the Surface Structure of l -Cysteine-Coated Ultrasmall Gold Nanoparticles (1.8 nm)
    In: Langmuir Vol. 35 (2019) Nr. 3, pp. 767 - 778
    Online Full Text: dx.doi.org/
  • Blüggel, Mike; van den Boom, Johannes; Meyer, Hemmo; Bayer, Peter; Beuck, Christine
    Structure of the PUB Domain from Ubiquitin Regulatory X Domain Protein 1 (UBXD1) and Its Interaction with the p97 AAA+ ATPase
    In: Biomolecules Vol. 9 (2019) Nr. 12, 876
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Hogeweg, Anna; Sowislok, Andrea; Schrader, Thomas; Beuck, Christine
    An NMR Method To Pinpoint Supramolecular Ligand Binding to Basic Residues on Proteins
    In: Angewandte Chemie International Edition Vol. 56 (2017) Nr. 46, pp. 14758 - 14762
    Online Full Text: dx.doi.org/
  • Trusch, Franziska; Kowski, Klaus; Bravo-Rodriguez, Kenny; Beuck, Christine; Sowislok, Andrea; Wettig, Burkhard; Matena, Anja; Sánchez-García, Elsa; Meyer, Hemmo; Schrader, Thomas; Bayer, Peter
    Molecular tweezers target a protein-protein interface and thereby modulate complex formation
    In: Chemical Communications: ChemComm Vol. 52 (2016) Nr. 98, pp. 14141 - 14144
    Online Full Text: dx.doi.org/ (Open Access)
  • van den Boom, Johannes; Trusch, Franziska; Hoppstock, Lukas; Beuck, Christine; Bayer, Peter
    Structural Characterization of the Loop at the Alpha-Subunit C-Terminus of the Mixed Lineage Leukemia Protein Activating Protease Taspase1
    In: PLoS ONE Vol. 11 (2016) Nr. 3, pp. e0151431
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Beuck, Christine; Williamson, James R.; Wüthrich, Kurt; Serrano, Pedro
    The acidic domain is a unique structural feature of the splicing factor SYNCRIP
    In: Protein Science Vol. 25 (2016) Nr. 8, pp. 1545 - 1550
    Online Full Text: dx.doi.org/ (Open Access)
  • Beuck, Christine; Weinhold, Elmar
    Reversibly locked thionucleobase pairs in DNA to study base flipping enzymes
    In: Beilstein Journal of Organic Chemistry Vol. 10 (2014) pp. 2293 - 2306
    Online Full Text: dx.doi.org/ (Open Access)