According to Bold's Basal Medium - BB - (CCAP); with addition of vitamins.

Preparation of Culture Solution

Dissolve stock solutions as indicated above and make up to 1000 ml of dH₂O. pH of the final medium should be around pH 6.6.

Titriplex II  -  Ethylenediaminetetraacetic acid  -  C₁₀H₁₆N₂O₈

Growth Conditions

If not otherwise stated, growth temperature is +/- 15^oC; light-dark-regime 14:10 h; light-intensity 5-15 µmol photons /m²/sec.
Media are made up to 1 litre with dH₂O or filtered seawater, as indicated.

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