Growth media - Central Collection of Algal Cultures (CCAC)
Culture Medium BBM
According to Bold's Basal Medium - BB - (CCAP); with addition of vitamins.
Preparation of Culture Solution
Dissolve stock solutions as indicated above and make up to 1000 ml of dH2O. pH of the final medium should be around pH 6.6.
Titriplex II - Ethylenediaminetetraacetic acid - C10H16N2O8
If not otherwise stated, growth temperature is +/- 15oC; light-dark-regime 14:10 h; light-intensity 5-15 µmol photons /m2/sec.
Media are made up to 1 litre with dH2O or filtered seawater, as indicated.