Growth media - Central Collection of Algal Cultures (CCAC)
Culture Medium L1
According to: Guillard, R.R.L. and Hargraves, P.E. 1993. Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia 32: 234-236.
Preparation of Culture Solution
Add the stock solutions as indicated above to 990 ml of filtered seawater and make up to 1 litre. The final pH should be 8.0 - 8.2.
L1 - Modifications
L/2 = L1 - Medium : filtered seawater = 1 : 1
1/2 L1 = L1 - Medium : dH2O = 1 : 1
If not otherwise stated, growth temperature is +/- 15oC; light-dark-regime 14:10 h; light-intensity 5-15 µmol photons /m2/sec.
Media are made up to 1 litre with dH2O or filtered seawater, as indicated.