Growth media - Central Collection of Algal Cultures (CCAC)
Synthetic Freshwater Medium - SFM
Preparation of Culture Medium
Add stock solutions 1-7 (or 1-8) to 950 ml of dH2O, adjust the pH to 7.0 (SFM) or 6.0 (SFM pH 6).
Bring the final volume to 1 litre and autoclave.
If not otherwise stated, growth temperature is +/- 15oC; light-dark-regime 14:10 h; light-intensity 5-15 µmol photons /m2/sec. Media are made up to 1 litre with dH2O or filtered seawater, as indicated.