Publications of the Aquatic Ecosystem Research group


70. Elbrecht V, Peinert B, Leese F.  (2017) Sorting things out: Assessing effects of unequal specimen biomass on DNA metabarcoding. Ecol Evol.;7:6918–6926.

69. Adrienne Jochum, Alexander M. Weigand, Estee Bochud, Thomas Inäbnit, Dorian D. Dörge, Bernhard Ruthensteiner, Adrien Favre, Gunhild Martels, Marian Kampschulte (2017) Three new species of Carychium O.F. Müller, 1773 from the Southeastern USA, Belize and Panama are described using computer tomography (CT) (Eupulmonata, Ellobioidea, Carychiidae). ZooKeys, doi:10.3897/zookeys.675.12453.

68. Mock T et al. [...] Leese, F, [...] & Grigoriev IV (2017) Evolutionary genomics of the cold-adapted diatom Fragilariopsis cylindrus. Nature 541: 536-540.

67. Bruce NL, Brix S, Balfour N, Kihara TC, Weigand AM, Mehterian S & Iliffe TM (2017) A new genus for Cirolana troglexuma Botosaneanu & Iliffe, 1997, an anchialine cave dwelling cirolanid isopod (Crustacea, Isopoda, Cirolanidae) from the Bahamas. Subterranean Biology 21: 57-92.

66. Macher JN, Zizka V, Weigand AM & Leese F (2017) A simple centrifugation protocol leads to a 55-fold mitochondrial DNA enrichment and paves the way for future mitogenomic research. bioRxiv doi:



65. Leese F et al. / DNAqua-Net consortium (2016) DNAqua-Net: Developing new genetic tools for bioassessment and monitoring of aquatic ecosystems in Europe. Research Ideas & Outcomes 2: e11321.

64. Macher JN, Weiss M, Beermann A & Leese F (2016) Cryptic diversity and population structure at small scales: the freshwater snail Ancylus (Planorbidae, Pulmonata) in the Montseny mountain range. International Journal of Limnology 52: 387-399.

63. Weiss M & Leese F (2016) Widely distributed and regionally isolated! Drivers of genetic structure in Gammarus fossarum in a human-impacted landscape. BMC Evolutionary Biology 16: 153.

62. Romero PE, Weigand AM & Pfenninger M (2016) Positive selection on panpulmonate mitogenomes provide new clues on adaptations to terrestrial life. BMC Evolutionary Biology 16: 164.

61. Rozenberg A, Leese F, Weiss LC & Tollrian R (2016) Digital gene expression analysis with sample multiplexing and PCR duplicate detection: a straightforward protocol. Biotechniques 61: 26-32.

60. Melzer RR, Bursic M, Ceseña F, Dömel JS, Heß S, Landmann S, Metz M, Pfannkuchen M, Reed I & Meyer R (2016) High decapod diversity revealed by minimal-invasive, short-term survey of Brijuni marine protected area. Biodiversity and Conservation 25: 1559-1567.

59. Elbrecht V, Taberlet P, Dejean T, Valentini A, Usseglio-Polatera P, Beisel J, Coissac E, Boyer F & Leese F (2016) Testing the potential of a ribosomal 16S marker for DNA metabarcoding of insects. PeerJ 4: e1966.

58. Katouzian AR, Sari A, Macher JN, Weiss M, Saboori A, Leese FWeigand AM (2016) Drastic underestimation of amphipod biodiversity in the endangered Irano-Anatolian and Caucasus biodiversity hotspots. Scientific Reports 6: 22507.

57. Weigand AM, Kremers J & Grabner D (2016) Shared microsporidian profiles between an obligate (Niphargus) and facultative subterranean amphipod population (Gammarus) at sympatry provide indications for underground transmission pathways. Limnologica 58: 7-10. 

56. Elbrecht V, Beermann AJ, Goessler G, Neumann J, Tollrian R, Wagner R, Wlecklik A, Piggott JJ, Matthaei CD & Leese F (2016) Multiple‐stressor effects on stream invertebrates: a mesocosm experiment manipulating nutrients, fine sediment and flow velocity. Freshwater Biology 61: 362-375.

55. Dambach J, Raupach MJ, Leese F, Schwarzer J & Engler J (in press) Ocean currents determine functional connectivity in an Antarctic deep-sea shrimp. Marine Ecology



54. Jochum A, Slapnik R, Klussmann-Kolb A, Páll-Gergely B, Kampschulte M, Martels G, Vrabec M, Nesselhauf C & Weigand AM (2015) Groping through the Black Box of Variability: An integrative taxonomic and nomenclatural re-evaluation of Zospeum isselianum Pollonera, 1887 and allied species using new imaging technology (Nano-CT, SEM), conchological, histological and molecular data (Ellobioidea: Carychiidae). Subterranean Biology 16: 123-165.

53. Weiss JC, Leese F, Laforsch C & Tollrian R (2015) Dopamine is a key regulator in the signalling pathway underlying predator-induced defences in Daphnia. Proceedings of the Royal Society Series B 282: 20151440.

52. Macher J-N, Salis RK, Blakemore K, Tollrian R, Matthaei C & Leese F (2015) Multiple-stressor effects on stream invertebrates: DNA barcoding reveals contrasting responses of cryptic mayfly species. Ecological Indicators 61(2): 159-169.

51. Brand P, Ramirez S, Leese F, Quezada-Euan JG, Tollrian R & Eltz T (2015) Rapid evolution of chemosensory receptor genes in a pair of sibling species of orchid bees (Apidae: Euglossini). BMC Evolutionary Biology 15: 176.

50. Elbrecht V & Leese F (2015) Can DNA-based ecosystem assessments quantify species abundance? Testing primer bias and biomass - sequence relationships with an innovative metabarcoding protocol. PLOS ONE 10:e0130324

49. Grabner DS*, Weigand AM*, Leese F, Winking C, Hering D, Tollrian R & Sures B (2015) Invaders, natives and their enemies: distribution patterns of amphipods and their microsporidian parasites in the Ruhr Metropolis, Germany. Parasites & Vectors 8: 419. (*equal contributions)

48. Rozenberg A, Parida M, Leese F, Weiss L, Tollrian R & Manak RJ (2015) Transcriptional profiling of predator-induced phenotypic plasticity in Daphnia pulex. BMC Frontiers in Zoology 12: 18.

47. Dietz L, Arango CP, Dömel JS, Halanych K, Harder A, Held C, Mahon A, Mayer C, Melzer RR, Rouse G, Weis A, Wilson N & Leese F (2015) Regional differentiation and extensive hybridisation between mitochondrial clades of the Southern Ocean giant sea spider Colossendeis megalonyx. Royal Society Open Science 2: 140424.

46. Rozenberg A, Brand P, Rivera N, Leese F & Schubart CD (2015) Characterization of fossilized relatives of the White Spot Syndrome Virus in genomes of unrelated decapod crustaceans. BMC Evolutionary Biology 15: 142.

45. Dietz L, Pieper S, Seefeldt MA & Leese F (2015) Morphological and genetic data clarify the taxonomic status of Colossendeis robusta and C. glacialis (Chelicerata: Pycnogonida) and reveal overlooked biodiversity. Arthropod Systematics & Phylogeny 73: 107-128.

44. Doemel JS, Convey P & Leese F (2015) Genetic data support independent glacial refugia and open ocean barriers to gene flow for the Southern Ocean sea spider Austropallene cornigera (Möbius, 1902). Journal of Crustacean Biology 35: 480-490.

43. Gaebler S*, Medlin L & Leese F* (2015) A puzzle with many pieces: The genetic structure and diversity of Phaeocystis antarctica Karsten. European Journal of Phycology 50: 112-124.

42. Macher J, Rozenberg A, Pauls SU, Wagner R, Tollrian R & Leese F (2015) Assessing the phylogeographic history of the montane caddisfly Thremma gallicum: A comparison of sequence data from mitochondria and restriction-site associated DNA markers (RAD). Ecology and Evolution 5: 648-662.



41. Brix S, Svavarsson J & Leese F (2014) A multi-gene analysis reveals multiple highly divergent lineages of the isopod Chelator insignis (Hansen, 1916) south of Iceland. J. Polar Research 35: 225-240.

40. Schweyen H, Rozenberg A & Leese F (2014) Inserts of short random oligos (INTROs) help to detect PCR amplification bias and minimize allelic dropout effects in ddRAD studies. Biological Bulletin 227: 146-160.

39. Brix S, Leese F, Riehl T & Kihara T-C (2014) A new genus and new species of Desmosomatidae Sars, 1897 (Isopoda) from the east South-Atlantic abyss described by means of integrative taxonomy. Marine Biodiversity 45: 7-61.

38. Pauls S, Alp M, Balint, M, Bernabo P, Ciampor F, Ciamporova-Zatovicova Z, Finn D, Kohout J, Leese F, Lencioni V, Paz-Vinas I & Monaghan M (2014) Integrating molecular tools into freshwater ecology: developments and opportunities. Freshwater Biology 59: 1559-1576.

37. Raupach MJ, Bininda-Emonds O, Knebelsberger T, Laakmann S, Pfaender J & Leese F (2014) Phylogeographic analysis of the common sea slater Ligia oceanica (Crustacea: Isopoda) reveals two deep divergent mitochondrial lineages and a significant population structure. Biological Journal of the Linnean Society 112: 16-30.

36. Lampert K, Pasternak V, Brand P, Leese F & Eltz T (2014) 'Late' male sperm precedence in polyandrous wool-carder bees, Anthidium manicatum L., and its possible role in the evolution of male resource defense in Hymenoptera. Animal Behaviour 90: 211-217.

35. Elbrecht V, Feld CK, Gies M, Hering D, Sondermann M, Tollrian R & Leese F (2014) Genetic diversity and dispersal potential of the stonefly Dinocras cephalotes in a central European low mountain range. Freshwater Sciences 33: 181-192.

34. Weis A, Meyer R, Dömel J, Dietz L, Leese F & Melzer RR (2014) Pallenopsis patagonica (Hoek, 1881) - a species complex revealed by morphology and DNA barcoding with description of a new species of Pallenopsis Wilson, 1881. Zoological Journal of the Linnean Society 170: 110-131.

33. Weiss M, Macher JN, Seefeldt M & Leese F (2014) Molecular phylogenetics reveals even more overlooked diversity within the Gammarus fossarum species complex. Hydrobiologia 721: 165-184.



32. Elbrecht V, Poettker L, John U & Leese F (2013) The complete mitochondrial genome of the stonefly Dinocras cephalotes (Plecoptera, Perlidae). Mitochondrial DNA 26(3): 469-470.

31. Agrawal S, Leach E, Leese F & Held C (2013) Isolation and characterization of 10 polymorphic loci for the giant Antarctic isopod, Glyptonotus antarcticus. Conservation Genetic Resources 5: 963-965.

30. Kaiser S, Brandao S, Brix S, Barnes DKA, Bowden DA, Brenke N, Ingels J, Leese F, Linse K, Schiaparelli S, Arango CP, Badhe R, Bax N, Blazewicz-Paszkowycz M, Brandt A, Catarino AI, David B, De Ridder C, Dubois P, Ellingsen KE, Glover AG, Griffith HJ, Gutt J, Halanych KM, Havermans C, Held C, Janussen D, Lörz A-N, Pearce DA, Pierrat B, Riehl, T, Rose A, Sands CJ, Soler I Membrives A, Schüller M, Strugnell JM, Vanreusel A, Veit-Kähler G, Wilson N & Yasuhara M (2013) Pattern, process and vulnerability of Antarctic and Southern Ocean benthos – a decadal leap in knowledge and understanding. Marine Biology 160: 2295-2317.

29. Dambach J, Raupach M, Schwarzer J, Mayer C & Leese F (2013) Isolation and characterization of nine polymorphic microsatellites for the deep-sea shrimp species Nematocarcinus lanceopes (Crustacea:  Decapoda: Caridea) using enriched library and 454 data. BMC Research Notes 6:75.

28. Read B, Kegel J, Klute M, Kuo A, Lefebvre SC, Maumus F, Mayer C, Miller J, Allen A, Bidle K, Borodovsky M, Bowler C, Brownlee C, Claverie J-M, Cock M, de Vargas C, Elias M, Frickenhaus S, Gladyshev VN, Gonzalez K, Guda C, Hadaegh A, Herman E, Iglesias-Rodriguez D, Jones B, Lawson T, Leese F, Lin Y-C, Lindquist R, Lobanov A, Lucas S, Malik SB, Marsh ME, Mock T, Monier A, Moreau H, Mueller-Roeber B, Napier J, Ogata H, Parker M, Probert I, Quesneville H, Raines C, Rensing S, Riano-Pachon DM, Richier S, Rokitta S, Salamov A, Sarno AF, Schmutz J, Schroeder D, Shiraiwa Y, Soanes DM, Valentin K, van der Giezen M, Van der Peer Y, Vardi A, Verret F, Von Dassow P, Wheeler G, Williams B, Wilson W, Wolfe G, Wurch LL, Young J, Joel B. Dacks*, Charles F. Delwiche*, Sonya Dyhrman*, Glockner G, John U, Richards T, Worden A, Zhang X & Grigoriev IV (2013) Emiliania’s pan genome drives the phytoplankton’s global distribution. Nature 499: 209-213.

27. Dietz L, Krapp F, Hendrickx M, Arango C, Krabbe K, Spaak JM & Leese F (2013) Morphological and genetic data show that Colossendeis tenera Hilton, 1943 (Arthropoda: Pycnogonida) does not belong to the Colossendeis megalonyx Hoek, 1881 complex. Organisms, Diversity & Evolution 13: 151-162.

26. Stemmer K*, Burghardt I, Mayer C, Reinecke G, Tollrian R, Wägele H & Leese F* (2013) Species assignments within xeniid soft corals (Octocorallia; Alcyonacea) Morphological and genetic approaches. Organisms, Diversity & Evolution 13: 135-150.



25. Leese F, Brand B, Rozenberg A, Mayer C, Agrawal S, Dambach J, Dietz L, Dömel JS, Goodall-Copstake WP, Held C, Jackson JA, Lampert KP, Linse K, Macher JN, Nolzen J, Raupch MJ, Rivera N, Schubart CD, Striewski S, Tollrian R & Sands CJ  (2012) Exploring Pandora’s Box: Potential and pitfalls of low coverage genome surveys for evolutionary biology. PLoS ONE e7:40202.

24. Bernhard B, Lampert KP, Leese F, Mayer C & Tollrian R (2012) Schreckstoff evolution: Are shoals of the Eurasian minnow (Phoxinus phoxinus) formed by close kin? Journal of Fish Biology 80: 713-721.

23. Hui M, Kochzius M & Leese F (2012) Isolation and characterisation of nine microsatellite markers in the boring giant clam (Tridacna crocea) and cross-amplification in five other tridacnid species. Marine Biodiversity 42: 285-287.



22. Leese F & Held C (2011) Analyzing intraspecific genetic variation: A practical guide using mitochondrial DNA and microsatellites. Crustacean Issues Vol 19, Chapter 1.

21. Chen J, Dai F, Balakrishnan-Renuka A, Leese F, Schempp W, Schaller F, Hoffmann MM, Morosan-Puopolo G, Yusuf F, Bisschoff IJ, Chankiewitz V, Xue J, Chen J, Ying K & Brand-Saberi B (2011) Diversification and Molecular Evolution of ATOH8, a Gene Encoding a bHLH Transcription Factor. PLoS ONE 6:e23005.

20. Kurtenbach S, Mayer C, Pelz T, Hatt H, Leese F & Neuhaus EM (2011) Molecular evolution of a chordate specific family of G protein-coupled receptors. BMC Evolutionary Biology 11:234.

19. Brix S, Riehl T & Leese F (2011) First genetic data for species of the genus Haploniscus Richardson, 1908 (Isopoda: Asellota: Haploniscidae) from neighbouring deep-sea basins in the South Atlantic. Zootaxa 2838: 79-84.

18. Dietz L, Mayer C, Arango C & Leese F (2011) The mitochondrial genome of Colossendeis megalonyx supports a basal position of Colossendeidae within the Pycnogonida. Molecular Phylogenetics and Evolution 58: 523-528.

17. Aggarwal RK, Allainguillaume J, Bajay MM, Barthwal S, Bertolino P, Chauhan P, Consuegra S, Croxford A, Dalton DL, den Belder E, Díaz-Ferguson E, Douglas MR, Drees M, Elderson J, Esselink GD, Fernández-Manjarrés JF, Frascaria-Lacoste N, Gäbler-Schwarz S, Garcia de Leaniz C, Ginwal HS, Goodisman MA, Guo B, Hamilton MB, Hayes PK, Hong Y, Kajita T, Kalinowski ST, Keller L, Koop BF, Kotzé A, Lalremruata A, Leese F, Li C, Liew WY, Martinelli S, Matthews EA, Medlin LK, Messmer AM, Meyer EI, Monteiro M, Moyer GR, Nelson RJ, Nguyen TT, Omoto C, Ono J, Pavinato VA, Pearcy M, Pinheiro JB, Power LD, Rawat A, Reusch TB, Sanderson D, Sannier J, Sathe S, Sheridan CK, Smulders MJ, Sukganah A, Takayama K, Tamura M, Tateishi Y, Vanhaecke D, Vu NV, Wickneswari R, Williams AS, Wimp GM, Witte V & Zucchi MI, (2011) Permanent genetic resources added to Molecular Ecology Resources Database 1 August 2010-30 September 2010, Molecular Ecology Resources 11: 219-222.



16. Tollrian R & Leese F (2010) Ecological Genomics: Steps towards unraveling the genetic basis of inducible defenses in Daphnia. BMC Biology 8:51.

15. Spanier KI*, Leese F*, Mayer C, Colbourne JK, Gilbert D, Pfrender M & Tollrian R (2010) Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR. BMC Molecular Biology 11:50. (*equal contributions)

14. Leese F, Agrawal S & Held C (2010) Long-distance island hopping without dispersal stages: Transportation across major zoographic barriers in a Southern Ocean isopod. Naturwissenschaften 97: 583-594.

13. Raupach MJ, Thatje S, Dambach J, Rehm P, Misof B & Leese F (2010) Genetic homogeneity and circum-Antarctic distribution of two benthic shrimp species of the Southern Ocean, Chorismus antarcticus and Nematocarcinus lanceopes. Marine Biology 157: 1783-1797.

12. Mayer C, Leese F & Tollrian R (2010) Genome-wide analysis of tandem repeats in Daphnia pulex - a comparative approach. BMC Genomics 11:277.

11. Krabbe K*, Leese F*, Mayer C, Tollrian R & Held C (2010) Cryptic mitochondrial lineages in the widespread pycnogonid Colossendeis megalonyx Hoek, 1881 from Antarctic and Subantarctic waters. Polar Biology 33: 271-280.  (*equal contributions)



10. Carstensen D, Laudien J, Leese F, Arntz W & Held C (2009) Genetic variability, shell and sperm morphology suggest that the surf clams Donax marincovichi and D. obesulus are one species. Journal of Molluscan Studies 75: 381-390.

9.   Kraemer L, Beszteri B, Gäbler-Schwarz S, Held C, Leese F, Mayer C, Pöhlmann K & Frickenhaus S (2009) STAMP: Extensions to the STADEN sequence analysis package for high throughput interactive microsatellite marker design. BMC Bioinformatics 10: e41.

8.   Leese F & Held C (2008) Identification and characterization of microsatellites from the Antarctic isopod Ceratoserolis trilobitoides - nuclear evidence for cryptic species. Conservation Genetics 9: 1369-1372.

7.   Leese F, Kop A, Wägele JW & Held C (2008) Cryptic speciation in a benthic isopod from Patagonian and Falkland Island waters and the impact of glaciations on its population structure. BMC Frontiers in Zoology 5:e19.

6.   Leese F, Kop A, Agrawal S & Held C (2008) Isolation and characterization of microsatellite markers from the marine isopods Serolis paradoxa and Septemserolis septemcarinata (Crustacea: Peracarida). Molecular Ecology Resources 8: 818-821.

5.   Leese F, Mayer C & Held C (2008) Isolation of microsatellites from unknown genomes using known genomes as enrichment templates. Limnology and Oceanography: Methods 6: 412-426.

4.   Held C* & Leese F* (2007) The utility of fast evolving molecular markers for studying speciation in the Antarctic benthos. Polar Biology 30: 513-521.

3.   Leese F & Brenke N (2005) Chauliodoniscus coronatus sp. nov., a new deep-sea species from the Angola Basin (Asellota, Janiroidea, Haploniscidae). Organisms Diversity & Evolution 5: 189-201.

2.   Leese F & Wagner R (2005) The "Sericostoma-Problem" - molecular, chemotaxonomic, and autecological approaches (Trichoptera: Sericostomatidae). Lauterbornia 54: 161-163.

1.   Wagner R, Leese F & Panesar AR (2004) Aquatic dance flies from a small Himalayan mountain stream (Diptera: Empididae: Hemerodromiinae, Trichopezinae and Clinocerinae). Bonner Zoologische Beitraege 52: 3-32.