CRC 1093 - Principal Investigator Sebastian Schlücker
Area A: Chemical Ligands and Methods A9: Raman spectroscopic monitoring of protein recognition by supramolecular ligands
A9 develops nanodiagnostic assays for target proteins which are present as dimers or oligomers and exhibit multiple allosteric binding sites for partner proteins/peptides. Peptide-functionalized, Raman reporter-labeled metal nanoparticles (PNPs) selectively bind to these sites and induce PNP aggregation, monitored by surface-enhanced Raman scattering (SERS). Addition of supramolecular ligands leads to quantifyable changes. Label-free and site-specific detection of pro-tein recognition by supramolecular ligands in solution will be achieved by ultraviolet resonance Ra-man (UVRR) difference spectroscopy in combination with site-directed mutagenesis.Read more
|2012‐today||Full Professor (W3) of Physical Chemistry, University of Duisburg‐Essen|
|2008-2012||Associate Professor (W2) of Experimental Physics, University of Osnabrück|
|2002-2004||Postdoctoral Research Assistant with Dr. I.W. Levin, Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, MD, USA|
Scientific education and degrees
|2007||Habilitation and Venia legendi (Physical Chemistry) at the University of Würzburg, Mentor: Prof. Dr. W. Kiefer|
|1998‐2002||Ph.D. at the University of Würzburg with Prof. Dr. Wolfgang Kiefer|
|1993-1998||Study of Chemistry (Dipl.) at the University of Würzburg, Diploma thesis with Prof. Dr. Wolfgang Kiefer, Würzburg|
E. Cortes, W. Xie, J. Cambiasso, A. S. Jermyn, R. Sundararaman, P. Narang, S. Schlücker, S. A. Maier: Plasmonic hot electron transport drives nano-localized chemistry. Nat. Commun., 2017, 8, 14880.
W. Xie, R. Grzeschik, S. Schlücker: Metal Nanoparticle-Catalyzed Reduction Using Borohydride in Aqueous Media: A Kinetic Analysis of the Surface Reaction by Microfluidic SERS. Angew. Chem. Int. Ed. 2016, 55, 13729-13733.
W. Xie, S. Schlücker: Hot electron-induced reduction of small molecules on photo-recycling metal surfaces. Nat. Commun. 2015, 7, 7570.
Y. Wang, M. Salehi, M. Schütz, S. Schlücker: Femtogram detection of cytokines in a direct dot-blot assay using SERS microspectroscopy and hydrophilically stabilized Au–Ag nanoshells. Chem. Comm. 2014, 50, 2711-2714.
W. Xie, B. Walkenfort, S. Schlücker: Label‐Free SERS Monitoring of Chemical Reactions Catalyzed by Small Gold Nanoparticles Using 3D Plasmonic Superstructures. J. Am. Chem. Soc. 2013, 135, 1657‐1660.
S. Niebling, H. Y. Kuchelmeister, C. Schmuck, S. Schlücker: Quantitative label‐free monitoring of peptide recognition by artificial receptors: a comparative FT‐IR and UV resonance Raman spectroscopic study. Chem. Sci. 2012, 3, 3371‐3377.
W. Xie, C. Herrmann, K. Kömpe, M. Haase, S. Schlücker: Synthesis of Bifunctional Au/Pt/Au Core/Shell Nanoraspberries for in Situ SERS Monitoring of Platinum‐Catalyzed Reactions. J. Am. Chem. Soc. 2011, 133, 19302‐19305.
B. Küstner, M. Gellner, M. Schütz, F. Schöppler, A. Marx, P. Ströbel, P. Adam, C. Schmuck, S. Schlücker: SERS labels for red laser excitation: silica‐encapsulated SAM on gold/silver nanoshells. Angew. Chem. Int. Ed. 2009, 48, 1950‐1953.
C. Schmuck, P. Wich, B. Küstner, W. Kiefer, S. Schlücker: Direct and label‐free detection of solidphase bound compounds by using surface enhanced Raman scattering (SERS) microspectroscopy. Angew. Chem. Int. Ed. 2007, 46, 4786 – 4789.