Analytics Core Facility Essen

Look into ACE --- left hand side

Look into ACE --- left side

From left to right: Exactive Plus EMR, Ultimate 3000, LCQ, HDx-Pal , Easy nLC 1200 and Orbitrap Fusion Lumos

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Look into ACE --- right hand side

Look into ACE --- right hand side

From left to right: column packing station, Evosep One, Easy nLC 1000, Orbitrap Elite, solvent cupboard, chemical hood

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Organization

The Analytics Core Facility Essen (ACE) is a central facility of the Center of Medical Biotechnology (ZMB) and of the Faculty of Biology of the University Duisburg-Essen. It offers access to state-of-the-art mass spectrometry-based proteomics for internal and external users. The timely technical equipment of the ACE allows us to provide a wide variety of different “bottom-up” and “top-down” proteomics methods. Furthermore, we also develop innovative solutions for project-specific demands. The service comprises all aspects of mass spectrometry-based proteomics, starting from technical advice and project planning, sample preparation and analysis to data analysis, data management and interpretation of results. We are also listed in the RIsources Poral of the DFG (RI_00466).

Contact

Address

Analytics Core Facility Essen (ACE)

Universität Duisburg-Essen
ZMB
Universitätsstr. 2
45141 Essen

https://www.uni-due.de/ace

ACE Core Facility Manager

Dr. Farnusch Kaschani

office: S03 S02 A62

MS-Labs: T03 R-1 C11/C14/C18

office: +49201 183 4924

MS-Lab: +49201 183 6839

Email

Management Board

Prof. Markus Kaiser
Prof. Michael Ehrmann
Prof. Peter Bayer
Prof. Hemmo Meyer
Dr. Farnusch Kaschani

Publications

    2022

  • Klaus, Thomas; Ninck, Sabrina; Albersmeier, Andreas; Busche, Tobias; Wibberg, Daniel; Jiang, Jianbing; Elcheninov, Alexander G.; Zayulina, Kseniya S.; Kaschani, Farnusch; Bräsen, Christopher; Overkleeft, Herman S.; Kalinowski, Jörn; Kublanov, Ilya V.; Kaiser, Markus; Siebers, Bettina
    Activity-Based Protein Profiling for the Identification of Novel Carbohydrate-Active Enzymes Involved in Xylan Degradation in the Hyperthermophilic Euryarchaeon Thermococcus sp. Strain 2319x1E
    In: Frontiers in Microbiology Vol. 12 (2022)
    ISSN: 1664-302X
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)

    • 2021

  • van Geelen, Lasse; Kaschani, Farnusch; Sazzadeh, Shabnam S.; Adeniyi, Emmanuel T.; Meier, Dieter; Proksch, Peter; Pfeffer, Klaus; Kaiser, Markus; Ioerger, Thomas R.; Kalscheuer, Rainer
    Correction to : Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteria
    In: Applied Microbiology and Biotechnology (2021) in press
    ISSN: 1432-0614; 0175-7598; 0171-1741
    Online Full Text: dx.doi.org/ (Open Access)
  • van den Boom, Johannes; Kück, Anja; Kravic, Bojana; Müschenborn, Helen; Giesing, Maike; Pan, Dongqing; Kaschani, Farnusch; Kaiser, Markus; Musacchio, Andrea; Meyer, Hemmo
    Targeted substrate loop insertion by VCP/p97 during PP1 complex disassembly
    In: Nature Structural & Molecular Biology Vol. 28 (2021) Nr. 12, pp. 964 - 971
    ISSN: 1545-9993; 1545-9985
    Online Full Text: dx.doi.org/
  • Dorsch, Madeleine; Kowalczyk, Manuela; Planque, Mélanie; Heilmann, Geronimo; Urban, Sebastian; Dujardin, Philip; Forster, Jan; Ueffing, Kristina; Nothdurft, Silke; Oeck, Sebastian; Paul, Annika; Liffers, Sven T.; Kaschani, Farnusch; Kaiser, Markus; Schramm, Alexander; Siveke, Jens; Winslow, Monte M.; Fendt, Sarah-Maria; Nalbant, Perihan; Grüner, Barbara M.
    Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis
    In: Cell Reports Vol. 37 (2021) Nr. 8,
    ISSN: 2211-1247
    Online Full Text: dx.doi.org/ (Open Access)
  • Abreha, Kibrom B.; Alexandersson, Erik; Resjö, Svante; Lankinen, Åsa; Sueldo, Daniela; Kaschani, Farnusch; Kaiser, Markus; van der Hoorn, Renier A. L.; Levander, Fredrik; Andreasson, Erik
    Article leaf apoplast of field-grown potato analyzed by quantitative proteomics and activity-based protein profiling
    In: International Journal of Molecular Sciences (IJMS) Vol. 22 (2021) Nr. 21,
    ISSN: 1422-0067; 1661-6596
    Online Full Text: dx.doi.org/ (Open Access)
  • van der Woude, Lennard; Piotrowski, Markus; Klaasse, Gruson; Paulus, Judith K.; Krahn, Daniel; Ninck, Sabrina; Kaschani, Farnusch; Kaiser, Markus; Novák, Ondřej; Ljung, Karin; Bulder, Suzanne; van Verk, Marcel; Snoek, Basten L.; Fiers, Martijn; Martin, Nathaniel I.; van der Hoorn, Renier A. L.; Robert, Stéphanie; Smeekens, Sjef; van Zanten, Martijn
    The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases
    In: The Plant Journal Vol. 106 (2021) Nr. 6, pp. 1523 - 1540
    ISSN: 1365-313X; 0960-7412
    Online Full Text: dx.doi.org/ (Open Access)

    • 2020

  • Smolin, Daniel; Tötsch, Niklas; Grad, Jean-Noël; Linders, Jürgen; Kaschani, Farnusch; Kaiser, Markus; Kirsch, Michael; Hoffmann, Daniel; Schrader, Thomas
    Accelerated trypsin autolysis by affinity polymer templates
    In: RSC Advances Vol. 10 (2020) Nr. 48, pp. 28711 - 28719
    ISSN: 2046-2069
    Online Full Text: dx.doi.org/ (Open Access)
  • Kracht, Matthias; van den Boom, Johannes; Seiler, Jonas; Kröning, Alexander; Kaschani, Farnusch; Kaiser, Markus; Meyer, Hemmo
    Protein Phosphatase-1 Complex Disassembly by p97 is Initiated through Multivalent Recognition of Catalytic and Regulatory Subunits by the p97 SEP-domain Adapters
    In: Journal of Molecular Biology (JMB) Vol. 432 (2020) Nr. 23, pp. 6061 - 6074
    ISSN: 1089-8638; 0022-2836
    Online Full Text: dx.doi.org/
  • Smolin, Daniel; Tötsch, Niklas; Grad, Jean-Noël; Linders, Jürgen; Kaschani, Farnusch; Kaiser, Markus; Kirsch, Michael; Hoffmann, Daniel; Schrader, Thomas
    Accelerated trypsin autolysis by affinity polymer templates
    In: RSC Advances Vol. 10 (2020) Nr. 48, pp. 28711 - 28719
    ISSN: 2046-2069
    Online Full Text: dx.doi.org/ (Open Access)
  • Bach-Pages, Marcel; Homma, Felix; Kourelis, Jiorgos; Kaschani, Farnusch; Mohammed, Shabaz; Kaiser, Markus; van der Hoorn, Renier A. L.; Castello, Alfredo; Preston, Gail M.
    Discovering the RNA-binding proteome of plant leaves with an improved RNA interactome capture method
    In: Biomolecules Vol. 10 (2020) Nr. 4,
    ISSN: 2218-273X
    Online Full Text: dx.doi.org/ (Open Access)
  • van Geelen, Lasse; Kaschani, Farnusch; Sazzadeh, Shabnam S.; Adeniyi, Emmanuel T.; Meier, Dieter; Proksch, Peter; Pfeffer, Klaus; Kaiser, Markus; Ioerger, Thomas R.; Kalscheuer, Rainer
    Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteria
    In: Applied Microbiology and Biotechnology Vol. 104 (2020) Nr. 13, pp. 5985 - 5998
    ISSN: 1432-0614; 0175-7598; 0171-1741; 0340-2118
    Online Full Text: dx.doi.org/ (Open Access)
  • Göhring, Anna; Michin, Irina; Gerdes, Tina; Schulze, Nina; Blüggel, Mike; Rehic, Edisa; Kaschani, Farnusch; Kaiser, Markus; Bayer, Peter
    Targeting of parvulin interactors by diazirine mediated cross-linking discloses a cellular role of human Par14/17 in actin polymerization
    In: Biological Chemistry Vol. 401 (2020) Nr. 8, pp. 955 - 968
    ISSN: 1437-4315; 1431-6730
    Online Full Text: dx.doi.org/ (Open Access)
  • Azizan, Asma; Kaschani, Farnusch; Barinas, Hellen; Blaskowski, Svenja; Kaiser, Markus; Denecke, Martin
    Using proteomics for an insight into the performance of activated sludge in a lab-scale WWTP
    In: International Biodeterioration & Biodegradation Vol. 149 (2020) pp. 104934
    ISSN: 0964-8305
    Online Full Text: dx.doi.org/
  • Krahn, Daniel; Heilmann, Geronimo; Vogel, Felix C. E.; Papadopoulos, Chrisovalantis; Zweerink, Susanne; Kaschani, Farnusch; Meyer, Hemmo; Rösch, Alexander; Kaiser, Markus
    Zelkovamycin is an OXPHOS Inhibitory Member of the Argyrin Natural Product Family
    In: Chemistry: A European Journal Vol. 26 (2020) Nr. 39, pp. 8524 - 8531
    ISSN: 1521-3765; 0947-6539
    Online Full Text: dx.doi.org/ (Open Access)

    • 2019

  • Weiss-Sadan, Tommy; Itzhak, Gal; Kaschani, Farnusch; Yu, Zhanru; Mahameed, Mohamed; Anaki, Adi; Ben-Nun, Yael; Merquiol, Emmanuelle; Tirosh, Boaz; Kessler, Benedikt; Kaiser, Markus; Blum, Galia
    Cathepsin L regulates metabolic networks controlling rapid cell growth and proliferation
    In: Molecular and Cellular Proteomics Vol. 18 (2019) Nr. 7, pp. 1330 - 1344
    ISSN: 1535-9476; 1535-9484
    Online Full Text: dx.doi.org/ (Open Access)
  • Weiss-Sadan, Tommy; Maimoun, David; Oelschlagel, Diana; Kaschani, Farnusch; Misiak, Danny; Gaikwad, Hanmant; Ben-Nun, Yael; Merquiol, Emmanuelle; Anaki, Adi; Tsvirkun, Darya; Kaiser, Markus; Michl, Patrick; Gotsman, Israel; Blum, Galia
    Cathepsins Drive Anti-Inflammatory Activity by Regulating Autophagy and Mitochondrial Dynamics in Macrophage Foam Cells
    In: Cellular Physiology and Biochemistry Vol. 53 (2019) Nr. 3, pp. 550 - 572
    ISSN: 1015-8987; 1421-9778
    Online Full Text: dx.doi.org/ (Open Access)
  • Kourelis, Jiorgos; Kaschani, Farnusch; Grosse-Holz, Friederike M.; Homma, Felix; Kaiser, Markus; van der Hoorn, Renier A. L.
    A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana
    In: BMC Genomics Vol. 20 (2019) Nr. 1, pp. 722
    ISSN: 1471-2164
    Online Full Text: dx.doi.org/ (Open Access)
  • Halder, Vivek; Suliman, Mohamed N S; Kaschani, Farnusch; Kaiser, Markus
    Plant chemical genetics reveals colistin sulphate as a SA and NPR1-independent PR1 inducer functioning via a p38-like kinase pathway
    In: Scientific Reports Vol. 9 (2019) Nr. 1, pp. 11196
    ISSN: 2045-2322
    Online Full Text: dx.doi.org/ (Open Access)
  • Jutras, Philippe V.; Grosse-Holz, Friederike; Kaschani, Farnusch; Kaiser, Markus; Michaud, Dominique; Hoorn, Renier A. L. van der
    Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor S/CYS8 in Nicotiana benthamiana
    In: Plant Biotechnology Journal Vol. 17 (2019) Nr. 8, pp. 1670 - 1678
    ISSN: 1467-7652; 1467-7644
    Online Full Text: dx.doi.org/ (Open Access)
  • Viñegra de la Torre, Natanael; Kaschani, Farnusch; Kaiser, Markus; van der Hoorn, Renier A L; Soppe, Wim J J; Misas Villamil, Johana C.
    Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds
    In: The Biochemical Journal Vol. 476 (2019) Nr. 5, pp. 843 - 857
    ISSN: 1470-8728
    Online Full Text: dx.doi.org/ (Open Access)
  • Schulze Hüynck, Jan; Kaschani, Farnusch; van der Linde, Karina; Ziemann, Sebastian; Müller, André N.; Colby, Thomas; Kaiser, Markus; Misas Villamil, Johana C.; Doehlemann, Gunther
    Proteases underground : Analysis of the maize root apoplast identifies organ specific papain-like cysteine protease activity
    In: Frontiers in Plant Science Vol. 10 (2019)
    ISSN: 1664-462X
    Online Full Text: dx.doi.org/ (Open Access)
  • Buscaill, Pierre; Chandrasekar, Balakumaran; Sanguankiattichai, Nattapong; Kourelis, Jiorgos; Kaschani, Farnusch; Thomas, Emma L.; Morimoto, Kyoko; Kaiser, Markus; Preston, Gail M.; Ichinose, Yuki; Van Der Hoorn, Renier A.L.
    Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides
    In: Science Vol. 364 (2019) Nr. 6436, pp. eaav0748
    ISSN: 1095-9203; 0036-8075
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Kraiselburd, Ivana; Brüls, Thomas; Heilmann, Geronimo; Kaschani, Farnusch; Kaiser, Markus; Meckenstock, Rainer
    Metabolic reconstruction of the genome of candidate Desulfatiglans TRIP_1 and identification of key candidate enzymes for anaerobic phenanthrene degradation
    In: Environmental Microbiology Vol. 21 (2019) Nr. 4, pp. 1267 - 1286
    ISSN: 1462-2912; 1462-2920
    Online Full Text: dx.doi.org/ (Open Access)
  • Morimoto, Kyoko; Cole, Kyle S.; Kourelis, Jiorgos; Witt, Collin H.; Brown, Daniel; Krahn, Daniel; Stegmann, Monika; Kaschani, Farnusch; Kaiser, Markus; Burton, Jonathan; Mohammed, Shabaz; Yamaguchi-Shinozaki, Kazuko; Weerapana, Eranthie; van der Hoorn, Renier A. L.
    Triazine probes target ascorbate peroxidases in plants
    In: Plant Physiology Vol. 180 (2019) Nr. 4, pp. 1848 - 1859
    ISSN: 1532-2548; 0032-0889
    Online Full Text: dx.doi.org/ (Open Access)