Chemical Ligands and Methods
A6: DNA-based nanocontainers for predesigned spatial confinement of proteins
In project A6 prefabricated hexagonal DNA-nanotubes of defined sizes will be equipped with recognition labels developed within the CRC and then loaded with selected HtrA proteases, whose biological role is strictly correlated to their oligomeric state. The altered protein properties will be investigated in detail. In the long run, this project will provide a general method which uses supramolecular chemistry for the spatial confinement of minimally modified proteins in engineered DNA-nanochambers, in order to dissect specific biochemical events on a single-molecule scale and generate biomaterials with tailored properties.