Biological Targets B7: Manipulation of the kinetochore-microtubule interface by supramolecular ligands

Error-free propagation of the genome from one cell generation to the next relies on kinetochores, large protein complexes that establish controlled interactions between chromosomes and the microtubule cytoskeleton. In project part B7 we will use supramolecular chemistry to reconstitute multivalent kinetochore-microtubule attachments in vitro and specifically perturb their function using tailored chemical compounds.  The interplay between multiprotein complexes and dynamic microtubule filaments will be visualized in real-time using single-molecule fluorescence microscopy. These strategies will facilitate a mechanistic analysis of force generation, microtubule-dynamics control and attachment-sensing by kinetochores.