ICCE Equipment - Andor/Nikon Spinning Disk confocal and TIRF

Confocal Systems Andor/Nikon Spinning Disk confocal and TIRF

Main Applications

  • confocal imaging (Spinning Disk), optical sectioning
  • fast multi-dimensional live cell + fixed specimen imaging
  • multi-position acquisition
  • FRAP, Photoactivation / Photoconversion
  • TIRF
  • epi
  • DIC

Technical Specifications

Microscope and Accessories

  • inverted Nikon Ti-E
  • Perfect Focus System PFS
  • motorized xy-stage
  • Prior Scientific NanoScanZ Piezo stage
  • external fluorescence lamp Nikon Intensilight
  • Halogen lamp for transmitted light
  • EMBLEM microscope incubator box with control device (temperature & CO2 control for live-cell imaging)

Lasers

Andor REVOLUTION 500 series AOTF laser modulator and combiner unit, suitable for launch into CSU/CSUX, TIRF or FRAPPA

  • Diode Laser cw 405 nm (100 mW)
  • Diode Laser cw 488 nm (50 mW)
  • Diode Laser cw 561 nm (50 mW)
  • Diode Laser cw 640 nm (100 mW)

Dual Spinning Disk Confocal Scanner

  • Yokogawa CSU-X1 Spinning Disk unit, max. 5000 rpm disk spin speed/1000 frames per second
  • Andor Revolution FRAP and Photo Activation illumination system (FRAPPA)

Cameras

  • Andor Technology iXon DU-897 monochrome backilluminated EMCCD camera, 512 x 512 imaging array, 16 x 16-µm pixels, 14-bit digitization (for Spinning Disk, TIRF)
  • Andor Technology Clara camera interline-transfer CCD camera, 1392 x 1040 imaging array, 6.45 x 6.45-µm pixels, 14-bit digitization (for widefield, TIRF)

Objectives

name

magnification

NA immersion
CFI Plan Fluor 10x/0.3 10x 0.3 dry
CFI Pl Apo VC 20x/0.75 20x 0.75 dry
CFI Pl Apo 40x/0.95 40x 0.95 dry
CFI Apo TIRF 60x/1.49 60x 1.49 oil
CFI Apo TIRF 100x/1.49 100x 1.49 oil

Fluorescence filters & Dichroics

for confocal imaging (emission discrimination and filters):
  • CSU Quad Dichroic mirror set for laser wavelength 405 nm, 488 nm, 561 nm, 640 nm
  • single band emission filters:
    • DAPI: BP 447/60 nm (Semrock BrightLine)
    • RFP: BP 617/73 nm (Semrock BrightLine)
    • Cy5: BP 685/40 nm (Semrock BrightLine)
  • dual band emission filters:
  • GFP/RFP: BP 512/23 nm + BP 630/91 nm (Semrock BrightLine)
  • GFP/Cy5: BP 538/50 nm + BP 685/45 nm (Semrock BrightLine)
for TIRF imaging (488 nm and 561 nm only):
  • Imaging with Andor iXON DU-897
    • TIRF Dual Line Beamsplitter zt 488/561rpc (use together with emission filters of CSU)
  • Imaging with Andor Clara
    • GFP: Beamsplitter zt 491 RDCXT, BrightLine HC 525/45
    • RFP: Beamsplitter zt 561 RDCXT, BrightLine HC 509/54
for widefield imaging (filter sets):
  • DAPI: EX 340-380, DM 400, BA 435-485
  • FITC: EX 465-495, DM 505, BA 515-555
  • TRITC: EX 540/25, DM 565, BA 605/55

Software

Andor IQ3

Location

S03 V04 F54

Contact

ICCE Manager

Dr. Nina Schulze

- on maternity leave -

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Nina.Schulze@uni-due.de

Dr. Nina Schulze

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Johannes.Koch@uni-due.de