ICCE Equipment - Andor/Nikon Spinning Disk confocal

Confocal SystemsAndor/Nikon Spinning Disk confocal

Room: S03 V04 F54

Main Applications

  • confocal imaging (Spinning Disk), optical sectioning
  • fast multi-dimensional live cell + fixed specimen imaging
  • multi-position acquisition
  • FRAP, Photoactivation / Photoconversion
  • epi
  • DIC

Funding

Andor/Nikon Spinning Disk confocal  microscope was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 181168673.

 

Technical Specifications

Microscope and Accessories

  • inverted Nikon Ti-E
  • Perfect Focus System PFS
  • motorized xy-stage
  • Prior Scientific NanoScanZ Piezo stage
  • external fluorescence lamp Nikon Intensilight
  • Halogen lamp for transmitted light
  • EMBLEM microscope incubator box with control device (temperature & CO2 control for live-cell imaging)

Lasers

Andor 4 line ILE multimode

  • Solid state 405nm smart diode laser (100 mW)
  • Solid state 488nm smart diode laser (50 mW)
  • Solid state 561nm smart diode laser (50 mW)
  • Solid state 637nm smart diode laser (140 mW)

Dual Spinning Disk Confocal Scanner

  • Yokogawa CSU-X1 Spinning Disk unit, max. 5000 rpm disk spin speed/1000 frames per second
  • Andor MicroPoint 4 Photostimulation & Optogenetics Tool

Cameras

  • Andor Technology ZL 41 Cell sCmos, 4.1 Megapixel, up to 100 fps, up to 100 nm resolution, up to 82% QE

Objectives

name

magnification

 NA immersion
CFI Plan Apo 60x/1.2 60x 1.2 water
CFI Pl Apo VC 20x/0.75 20x 0.75 dry
CFI Pl Apo 40x/0.95 40x 0.95 dry
CFI Apo TIRF 60x/1.49 60x 1.49 oil
CFI Plan Apo Lambda S100x/1.35 100x 1.35 silicone oil

Fluorescence filters & Dichroics

for confocal imaging (emission discrimination and filters):
  • CSU Quad Dichroic mirror set for laser wavelength 405 nm, 488 nm, 561 nm, 640 nm
  • single band emission filters:
    • DAPI: BP 447/60 nm (Semrock BrightLine)
    • RFP: BP 617/73 nm (Semrock BrightLine)
    • Cy5: BP 685/40 nm (Semrock BrightLine)
  • dual band emission filters:
  • GFP/RFP: BP 512/23 nm + BP 630/91 nm (Semrock BrightLine)
  • GFP/Cy5: BP 538/50 nm + BP 685/45 nm (Semrock BrightLine)
for widefield imaging (filter sets):
  • DAPI: EX 340-380, DM 400, BA 435-485
  • FITC: EX 465-495, DM 505, BA 515-555
  • TRITC: EX 540/25, DM 565, BA 605/55

 

Software

Andor IQ3