ICCE Equipment - Zeiss AxioObserver Z.1 microinjection

Widefield Systems Zeiss AxioObserver Z.1 microinjection

Main Applications

  • widefield system for epi-fluorescence and transmitted light applications (brightfield)
  • multi-dimensional live cell + fixed specimen imaging
  • computer-assisted microinjection

Technical Specifications

Microscope and Accessories

  • inverted Zeiss AxioObserver Z1
  • motorized xy-stage
  • external fluorescence lamp, mercury burner (100 W)
  • Halogen lamp for transmitted light
  • Micromanipulation unit: LN manipulator block xyz, motorized for microinjection; AIS 2 FemtoJet
  • EMBLEM microscope incubator box with control device (temperature & CO2 control for live-cell imaging)

Cameras

  • The Imaging Source DMK31BF03 CCD camera, 1024 x 768 imaging array, 4.65 x 4.65-µm pixels, 8-bit digitization
  • Zeiss AxioCam MRm CCD camera, 1388 x 1040 imaging array, 6.45 x 6.45-µm pixels, 12-bit digitization

Objectives

name

magnification

NA immersion
A-Plan 5x/0.12 5x 0.2 dry
LD Plan-Neofluar 20x/0.4 Corr Ph2 M27 20x 0.4 dry
LD Plan-Neofluar 40x/0.6 Corr Ph2 M27 40x 0.6 dry
Plan-Apochromat 63x/1.40 Oil 63x 1.4 oil

Fluorescence filter sets

  • Set 01 UV: Ex BP 365/12, FT 395, Em LP 397 (e.g DAPI, Hoechst)
  • Set 10: Ex BP 450-490, FT 510, Em BP 515-565 (e.g. EGFP, Alexa Fluor 488, FITC)
  • Set 15: Ex BP 546/12, FT 580, Em LP 590 (e.g. TAMRA, Rhodamine, Alexa Fluor 555)
  • Set 26: Ex BP 575-625, FT 645, Em BP 660-710 (e.g mPlum, Nile Red)

Software

Zeiss ZEN 2.0

Location

S05 V04 E65

Contact

ICCE Manager

Dr. Nina Schulze

- on maternity leave -

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Nina.Schulze@uni-due.de

Dr. Johannes Koch

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Johannes.Koch@uni-due.de