DeltaVision Elite high resolution microscope

Widefield Systems DeltaVision Elite high resolution microscope

Main Applications

  • widefield deconvolution fluorescence microscope
  • transmitted light applications (brightfield, DIC)
  • multi-dimensional live cell + fixed specimen imaging
  • multi-position acquisition
  • Low light imaging of sensitive samples

Technical Specifications

Microscope and Accessories

  • Inverted Olympus IX-71 microscope stand
  • UltimateFocus Modul (hardware autofocus system)
  • Automated high precision Flexure stage (long travel, 25x50 mm), “3D multisite visiting”
  • Environmental chamber with temperature & CO2 control for live cell imaging

Camera

  • pco.edge 5.5 sCMOS camera, 2560 x 2160 pixels, 6.5 x 6.5 µm pixel size, 16.6 x 14 mm sensor format, 16-bit digitization, 200 fps at 1024 x 1024

Light sources

  • DV Light SSI 7 color ultra-fast solid state illumination

    excitation filters

Fluorochrome excitation bandpass [nm]
DAPI 390/18
CFP 438/24
GFP/TRITC 475/28
YFP 513/17
TRITC 542/27
mCherry/Alexa594 575/25
Cy5 632/22
  • LED transmitted light module

Emission filters & Polychroic beamsplitters

Single band emission filters

Fluorochrome emission bandpass [nm]
DAPI 435/48 (use with Polychroic #1 or #2)
CFP 475/24 (use with Polychroic #3)
GFP/TRITC 525/48 (use with Polychroic #1 or #2)
YFP 548/22 (use with Polychroic #3)
TRITC 597/45 (use with Polychroic # 1)
mCherry/Alexa594 625/45 (use with Polychroic #2 or #3)
Cy5 679/37 (use with Polychroic #1 or #2)

Polychroic beamsplitters

Position Fluorochromes
Polychroic #1       DAPI/FITC/TRITC/Cy5
Polychroic #2 DAPI/FITC/mCherry/Alexa594/Cy5
Polychroic #3 CFP/YFP/mCherry

Objectives

name

magnification

NA immersion
Super-Plan Apo 20x/0.75 DIC 20x 0.75 dry
Plan Apo 60x/1.42 oil DIC 60x 1.42 oil
Super-Plan Apo 100x/1.4 oil DIC 100x 1.4 oil

Software

  • SoftWoRx 6 and Resolve 3D (integrated in SoftWoRx) with software modules for Deconvolution (image restoration), Cell tracking, Point visiting, Optic axial integration (OAI) and 3D visualization
Read more
ZMB

Location

S05 R01 H24

Contact

Dr. Nina Schulze

Phone: +49201 183 2622

Room: S05 V05 F48

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Nina.Schulze@uni-due.de

Dr. Johannes Koch

Phone: +49201 183 2622

Room: S05 V05 F48

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Johannes.Koch@uni-due.de