ICCE Equipment - Leica TCS SP8X Falcon

Confocal Systems Leica TCS SP8X Falcon

Main Applications

  • confocal (point) laser scanning microscopy, optical sectioning
  • FLIM
  • F(C)CS, FLCS
  • laser microirradiation (e.g. DNA damage) via UVA laser ablation system (355 nm)
  • Photoactivation / Photoconversion
  • Confocal super-resolution with Leica Lightning (Detection and Image Information Extraction/ Deconvolution for “confocal super-resolution” down to 120 nm)
  • FRAP
  • FRET (FLIM-FRET, acceptor photobleaching & sensitized emission)
  • λ2 Excitation-Emission scans
  • multi-dimensional live cell + fixed specimen imaging
  • multi-position, tile scan / Mosaic
  • automated imaging & screening (Leica HCS A, Leica Navigator)

Technical Specifications

Microscope and Accessories

  • inverted Leica DMi8 CS
  • Leica Adaptive Focus Control (AFC)
  • motorized xy-stage
  • SuperZ Galvo
  • SuperZ stage accessories: Microtiterplate insert, adapter for petri dishes (36 mm dishes) and universal sample holder
  • external fluorescence lamp LQ-HXP 120 LEJ
  • LED lamp for transmitted light and equipment for confocal DIC
  • controlled environmental conditions for live cell imaging (temperature, CO2)

Lasers

  • continuous wave lasers
    • Diode Laser (50 mW): 405 nm DMOD
    • Argon Laser (65 mW): 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
  • pulsed lasers
    • WLL E laser + pulsepicker: 470 nm to 670 nm, avg. power 1 mW, integrated pulsepicker: 80 MHz, 40 MHz, 20 MHz, 10 MHz
  • Laser ablation system (UVA laser)
    • UGA-42 Caliburn 355/42 (Rapp OptoElectronic), 355 nm

Scanners

  • non-resonant (conventional/variable) Scanner: 1 – 1800 Hz (7 fps @ 512 x 512 px, 84 fps @ 512 x 16 px), line frequency up to 3600 lines/second (bidirectional), max. scan format/image resolution 8192 x 8192 px (64 Mpixel), scanfield 22 mm (SFZ)
  • resonant Scanner (ultra-fast, reduced photodamage): 8000 Hz (28 fps @ 512 x 512 px, 290 fps @ 512 x 16 px), line frequency up to 16000 lines/second (bidirectional), max. scan format/image resolution 1248 x 1248 px (> 1 Mpixel), scanfield 13 mm (SFZ)

Detector System

  • 5-channel SP-Detector: 2 HyDs SMD, 1 HyD, 2 PMTs
  • 1 PMT for transmitted light (PMT trans)

Beam splitting between excitation and emission light is controlled via AOBS.

Objectives

name

magnification

NA immersion
HC PL APO 20x/0.75 CS2 20x 0.75 dry
HC PL APO 63x/1.2 W motCORR CS2 63x 1.2 water
HC PL APO 63x/1.3 GLYC CORR CS2 63x 1.3 glycerol
HC PL APO 63x/1.4 Oil CS2 63x 1.4 oil

Filter sets for LQ HXP-120

  • Filterset DAPI (Excitation BP 350/50, Dichroic 400, Emission BP 460/50)
  • Filterset FITC (Excitation BP 480/40, Dichroic 505, Emission BP 527/30)
  • Filterset RHOD (Excitation BP 546/10, Dichroic 560, Emission BP 585/40)

Software

  • Main CUDA workstation equipped with software modules:
    • LAS X Core
    • LAS X FCS (acquisition and analysis of FCS, F(C)CS, and FLCS data)
    • LAS X Lightning Expert (detection and Image Information Extraction/ Deconvolution for “confocal super-resolution” down to 120 nm)
    • LAS X Navigator (fast overviews, automated image acquisition)
    • HCS-A Matrix Screener (for automated microscopy, high content screening)
    • LAS X Live Data Mode (Interactive data recording allowing job-sequencing and online evaluation)
    • LAS X MicroLab (FRET, FRAP, FLIP)
      • FRAP wizard, FRAP XT wizard
      • FRET wizard (acceptor photobleaching & sensitized emission)
    • LAS X 3D Visualization
    • LAS X Dye Finder (Multi-color restoration, channel unmixing)
    • LAS X Environmental control (full control of your experimental conditions, log of the environmental data and monitoring during experiment)
    • Rapp OptoElectronic SysCon2 (control and setup of UVA laser ablation system and connection to LAS X via LocalCam plugin)
    Offline workstation equipped with software modules:
    • LAS X Basic License
    • LAS X FLIM offline analysis
    • LAS X FCS offline analysis
    • LAS X 3D Visualization

Location

S05 T04 B97

Contact

ICCE Manager

Dr. Nina Schulze

- on maternity leave -

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Nina.Schulze@uni-due.de

Dr. Johannes Koch

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Johannes.Koch@uni-due.de