ICCE Equipment - Zeiss Axio Observer 7
Widefield Systems Zeiss Axio Observer 7
Room: S05 T04 B97
Main Applications
- widefield system for epi-fluorescence applications
- multi-dimensional imaging of fixed specimen
Technical Specifications
Microscope and Accessories
- inverted Zeiss Axio Observer 7
- Colibri 7 multi-color LED illumination for fluorescence: 385/430/475/511/555/590/630 nm
- microLED for transmitted light
- mechanical stage 130x85R
- LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC, DIC; 6 positions, mot.
Cameras
Zeiss AxioCam 506 mono CCD camera, 2752 x 2208 imaging array, 4.54 µm x 4.54 µm pixels, 14-bit digitization
Objectives
| name |
magnification |
NA | immersion |
| EC Plan-Neofluar 10x/0.3 | 10x | 0.3 | dry |
| Plan-Neofluar 20x/0,50 | 20x | 0.5 | dry |
| LD Achroplan 20x/0.40 corr. Ph 2 | 20x | 0.4 | dry |
| EC Plan-Neofluar 40x/0.75 | 40x | 0.75 | dry |
| Plan-Neofluar 40x/1.3 Oil DIC | 40x | 1.3 | oil |
| C Plan-Apochromat 63x/1.4 Oil DIC | 63x | 1.4 | oil |
Fluorescence filter sets
- Set 01 UV: Ex BP 365/12, FT 395, Em LP 397 (e.g. DAPI, Hoechst)
- Set 47: Ex BP 436/20, FT 455, Em BP 480/40 (e.g. ECFP)
- Set 38 HE: Ex BP 470/40, FT 495, Em BP 525/50 (e.g. EGFP, Alexa Fluor 488, FITC)
- Set 46: Ex BP 500/20, FT 515, Em BP 535/30 (e.g. EYFP, Alexa Fluor 488, Citrine)
- Set 15: Ex BP 546/12, FT 580, Em LP 590 (e.g. TAMRA, Rhodamine, Alexa Fluor 555)
- Set 50: Ex BP 640/30, FT 660, Em BP 690/50 (e.g. Cy5, Alexa Fluor 647, Atto 647)
Software
ZEN 2.3 (Zeiss) with software modules Measurement, Multi Channel, Panorama, Manual Extended Focus, Z-Stack, Extended Focus and Image Analysis