ICCE Equipment - Zeiss Axio Observer 7

Widefield Systems Zeiss Axio Observer 7

Main Applications

  • widefield system for epi-fluorescence applications
  • multi-dimensional imaging of fixed specimen

Technical Specifications

Microscope and Accessories

  • inverted Zeiss Axio Observer 7
  • Colibri 7 multi-color LED illumination for fluorescence: 385/430/475/511/555/590/630 nm
  • microLED for transmitted light
  • mechanical stage 130x85R
  • LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC, DIC; 6 positions, mot.

Cameras

Zeiss AxioCam 506 mono CCD camera, 2752 x 2208 imaging array, 4.54 µm x 4.54 µm pixels, 14-bit digitization

Objectives

name

magnification

NA immersion
EC Plan-Neofluar 10x/0.3 10x 0.3 dry
Plan-Neofluar 20x/0,50 20x 0.5 dry
LD Achroplan 20x/0.40 corr. Ph 2 20x 0.4 dry
EC Plan-Neofluar 40x/0.75 40x 0.75 dry
Plan-Neofluar 40x/1.3 Oil DIC 40x 1.3 oil
C Plan-Apochromat 63x/1.4 Oil DIC 63x 1.4 oil

Fluorescence filter sets

  • Set 01 UV: Ex BP 365/12, FT 395, Em LP 397 (e.g. DAPI, Hoechst)
  • Set 47: Ex BP 436/20, FT 455, Em BP 480/40 (e.g. ECFP)
  • Set 38 HE: Ex BP 470/40, FT 495, Em BP 525/50 (e.g. EGFP, Alexa Fluor 488, FITC)
  • Set 46: Ex BP 500/20, FT 515, Em BP 535/30 (e.g. EYFP, Alexa Fluor 488, Citrine)
  • Set 15: Ex BP 546/12, FT 580, Em LP 590 (e.g. TAMRA, Rhodamine, Alexa Fluor 555)
  • Set 50: Ex BP 640/30, FT 660, Em BP 690/50 (e.g. Cy5, Alexa Fluor 647, Atto 647)

Software

ZEN 2.3 (Zeiss) with software modules Measurement, Multi Channel, Panorama, Manual Extended Focus, Z-Stack, Extended Focus and Image Analysis

Location

S05 V-01 E98

Contact

ICCE Manager

Dr. Nina Schulze

- on maternity leave -

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Nina.Schulze@uni-due.de

Contact

Dr. Johannes Koch

Phone: +49201 183 2622

Room: S05 V04 F17

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Johannes.Koch@uni-due.de