ICCE Equipment - Olympus BX61

Widefield Systems Olympus BX61

Room: S03 V04 F54

Main Applications

  • widefield system for epi-fluorescence applications
  • multi-dimensional imaging of fixed specimen

Technical Specifications

Microscope and Accessories

  • upright Olympus BX61
  • external fluorescence lamp, mercury burner (100 W)
  • Halogen lamp for transmitted light

Camera

Olympus Soft Imaging Solutions F-View II CCD camera, 1376 x 1032 imaging array, 6.45 x 6.45-µm pixels, 12-bit digitization

Objectives

name

magnification

 NA immersion
PlanC N 10x/0.25 Ph1 10x 0.25 dry
U Plan Fl N 40x/0.75 40x 0.75 dry
U Plan Fl N 63x/1.25 oil Iris Ph3 63x 1.25 oil
U Plan S Apo 100x/1.4 oil 100x 1.4 oil

Fluorescence filter sets

  • DAPI: Ex BP 360-370, BS 395, Em 440/20 (e.g. DAPI, Hoechst
  • ECFP: Ex BP 436/20, BS 455, Em BP 480/40 (e.g. ECFP, Cerulean)
  • EGFP: Ex BP 480/20, BS 495, Em BP 510/20 (e.g. EGFP, Alexa Fluor 488, FITC)
  • EYFP: Ex BP 500/20, BS 515, Em BP 535/30 (e.g. EYFP, Alexa Fluor 488, Citrine)
  • TRITC: Ex BP 545/30, BS 570, Em 610/75 (e.g. TRITC, Cy3, Alexa Fluor 546)

 

Software

Olympus Cell^P