ZMB Member Robert Huber
ZMB Member
Robert Huber
Next ZMB-Member
Prof. Dr. Dr. h.c. mult. Robert Huber FRS
Group
Structure ResearchCenter of Medical Biotechnology
Room: S03 S02 A45
University of Duisburg-Essen
45141 Essen
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
- +49 201 183 4141
- Website
- Selected Publications
- Publication Metrics
- ZMB Research Program
Molecular and Chemical Cell Biology
Research Overview
Professional interests
Structure and function of biological macromolecules, in particular those of large complex aggregates.
Systems studied
- Proteases and their natural and synthetic inhibitors.
- Metalloenzymes (iron, nickel, molybdenum, copper).
- Proteins of the immune system (antibodies and antibody receptors).
- Protein hormones and their receptors.
- Protein kinases.
- Proteins of amino acid biosynthesis (PLP containing enzymes).
- Proteins of cofactor and vitamin biosynthesis.
- Proteins of energy and electron transport.
Methods development
- Patterson methods in crystallography.
- Methods of structure determination of proteins and protein ligand complexes by NMR.
- Synthesis and use of electron rich metal clusters.
- Crystal annealing and improvement, methods and instruments.
- Analysis and evaluation of targets for research and application in pharmacology and crop science.
Selected Publications
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Rational correction of pathogenic conformational defects in HTRA1In: Nature Communications Vol. 15 (2024) Nr. 1, 5944Online Full Text: dx.doi.org/ (Open Access)
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Facile Multicomponent Synthesis of Oxazolidinones from Primary Amines and Cesium (Hydrogen)CarbonateIn: European Journal of Organic Chemistry Vol. 26 (2023) Nr. 27, e202300135Online Full Text: dx.doi.org/ (Open Access)
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Hemophagocytic lymphohistiocytosis : Like hyperinflammation due to a de novo mutation in DPP9In: The Journal of Allergy and Clinical Immunology Vol. 152 (2023) Nr. 5, pp. 1336 - 1344.e5Online Full Text: dx.doi.org/
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Chemoproteomics‐Enabled Identification of 4‐Oxo‐β‐Lactams as Inhibitors of Dipeptidyl Peptidases 8 and 9In: Angewandte Chemie International Edition Vol. 61 (2022) Nr. 47, e202210498Online Full Text: dx.doi.org/ (Open Access)
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Dipeptidyl peptidase 9 triggers BRCA2 degradation and promotes DNA damage repairIn: EMBO Reports Vol. 23 (2022) Nr. 10, e54136Online Full Text: dx.doi.org/ (Open Access)
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Aerosol-based ligand soaking of reservoir-free protein crystalsIn: Journal of Applied Crystallography Vol. 54 (2021) Nr. 3, pp. 895 - 902Online Full Text: dx.doi.org/ (Open Access)
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Structural basis for the regulation of nucleosome recognition and HDAC activity by histone deacetylase assembliesIn: Science Advances Vol. 7 (2021) Nr. 2, pp. eabd4413Online Full Text: dx.doi.org/ (Open Access)
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A precisely positioned MED12 activation helix stimulates CDK8 kinase activityIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 117 (2020) Nr. 6, pp. 2894 - 2905Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Activation by substoichiometric inhibitionIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 117 (2020) Nr. 3, pp. 1414 - 1418Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Crystal structure and mechanism of human carboxypeptidase O : Insights into its specific activity for acidic residuesIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 115 (2018) Nr. 17, pp. E3932 - E3939Online Full Text: dx.doi.org/ (Open Access)
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Structures and mechanism of dipeptidyl peptidases 8 and 9, important players in cellular homeostasis and cancerIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 115 (2018) Nr. 7, pp. E1437 - E1445Online Full Text: dx.doi.org/ (Open Access)
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Structure of the unliganded form of the proprotein convertase furin suggests activation by a substrate-induced mechanismIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 113 (2016) Nr. 40, pp. 11196 - 11201Online Full Text: dx.doi.org/ (Open Access)
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Design, Synthesis, and Biological Evaluation of Quercetagetin Analogues as JNK1 InhibitorsIn: Chemistry - A European Journal Vol. 21 (2015) Nr. 47, pp. 16887 - 16894Online Full Text: dx.doi.org/
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Flt3 is a target of coumestrol in protecting against UVB-induced skin photoagingIn: Biochemical Pharmacology Vol. 98 (2015) Nr. 3, pp. 473 - 483Online Full Text: dx.doi.org/
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Cerebral small vessel disease-related protease HtrA1 processes latent TGF-β binding protein 1 and facilitates TGF-β signalingIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 111 (2014) Nr. 46, pp. 16496 - 16501Online Full Text: dx.doi.org/
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Differential global structural changes in the core particle of yeast and mouse proteasome induced by ligand bindingIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 111 (2014) Nr. 26, pp. 9479 - 9484Online Full Text: dx.doi.org/
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IR laser-induced protein crystal transformationIn: Acta Crystallographica Section D: Biological Crystallography Vol. D70 (2014) Nr. 5, pp. 1224 - 1232Online Full Text: dx.doi.org/ (Open Access)
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Structural analysis of the human fibroblast growth factor receptor 4 kinaseIn: Journal of Molecular Biology (JMB) Vol. 426 (2014) Nr. 22, pp. 3744 - 3756Online Full Text: dx.doi.org/
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Structural and Functional Analysis of the Natural JNK1 Inhibitor QuercetagetinIn: Journal of Molecular Biology (JMB) Vol. 425 (2013) Nr. 2, pp. 411 - 423Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Structure-kinetic relationship study of CDK8/CycC specific compoundsIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 110 (2013) Nr. 20, pp. 8081 - 8086Online Full Text: dx.doi.org/
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“How I Chose Research on Proteases or, More Correctly, How it Chose Me”In: Angewandte Chemie International Edition Vol. 52 (2013) Nr. 1, pp. 68 - 73Online Full Text: dx.doi.org/
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Chemical Biology Approaches Reveal Conserved Features of a C-Terminal Processing PDZ ProteaseIn: ChemBioChem Vol. 13 (2012) Nr. 3, pp. 402 - 408Online Full Text: dx.doi.org/
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Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregatesIn: The Journal of Biological Chemistry (JBC) Vol. 287 (2012) Nr. 25, pp. 20931 - 20941Online Full Text: dx.doi.org/
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Hydroxyureas as noncovalent proteasome inhibitorsIn: Angewandte Chemie International Edition Vol. 51 (2012) Nr. 1, pp. 247 - 249Online Full Text: dx.doi.org/
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Structure of the stapled p53 peptide bound to Mdm2In: Journal of the American Chemical Society: JACS Vol. 134 (2012) Nr. 1, pp. 103 - 106Online Full Text: dx.doi.org/
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Elucidation of the α-keto-aldehyde binding mechanism : A lead structure motif for proteasome inhibitionIn: Angewandte Chemie International Edition Vol. 50 (2011) Nr. 2, pp. 542 - 544Online Full Text: dx.doi.org/
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HTRA proteases : regulated proteolysis in protein quality controlIn: Nature Reviews Molecular Cell Biology Vol. 12 (2011) Nr. 3, pp. 152 - 162Online Full Text: dx.doi.org/
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Molecular architecture of the Spire-actin nucleus and its implication for actin filament assemblyIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 108 (2011) Nr. 49, pp. 19575 - 19580Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Protein quality control in the bacterial periplasmIn: Annual Review of Microbiology Vol. 65 (2011) pp. 149 - 168Online Full Text: dx.doi.org/
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The structure of CDK8/CycC implicates specificity in the CDK/cyclin family and reveals interaction with a deep pocket binderIn: Journal of Molecular Biology (JMB) Vol. 412 (2011) Nr. 2, pp. 251 - 266Online Full Text: dx.doi.org/
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Convergent synthesis and biological evaluation of syringolin a and derivatives as eukaryotic 20S proteasome inhibitorsIn: European Journal of Organic Chemistry (2010) Nr. 21, pp. 3991 - 4003Online Full Text: dx.doi.org/
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Conversion of a regulatory into a degradative proteaseIn: Journal of Molecular Biology (JMB) Vol. 397 (2010) Nr. 4, pp. 957 - 966Online Full Text: dx.doi.org/
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Determinants of structural and functional plasticity of a widely conserved protease chaperone complexIn: Nature Structural & Molecular Biology Vol. 17 (2010) Nr. 7, pp. 837 - 843Online Full Text: dx.doi.org/
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HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cuesIn: Nature Structural & Molecular Biology Vol. 17 (2010) Nr. 7, pp. 844 - 852Online Full Text: dx.doi.org/
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Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleationIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 107 (2010) Nr. 26, pp. 11757 - 11762Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3In: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 106 (2009) Nr. 40, pp. 17223 - 17228Online Full Text: dx.doi.org/
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Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coliIn: Research in Microbiology Vol. 160 (2009) Nr. 9, pp. 660 - 666Online Full Text: dx.doi.org/
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Synthetic and structural studies on Syringolin A and B reveal critical determinants for selectivity and potency of proteasome inhibitionIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 106 (2009) Nr. 16, pp. 6507 - 6512
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Syringolin A selectively labels the 20S proteasome in murine EL4 and wildtype and bortezomib adapted leukemic cell linesIn: ChemBioChem Vol. 10 (2009) Nr. 16, pp. 2638 - 2643Online Full Text: dx.doi.org/ Online Full Text
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A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanismIn: Nature Vol. 452 (2008) pp. 755 - 758
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Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteinsIn: Proceedings of the National Academy of Sciences of the United States of America (PNAS) Vol. 22 (2008) pp. 7702 - 7707
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Binding mode of TMC-95A analogues to eukaryotic 20S proteasomeIn: ChemBioChem Vol. 5 (2004) Nr. 9, pp. 1256 - 1266Online Full Text: dx.doi.org/
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Navigation inside a protease: Substrate selection and product exit in the tricorn protease from Thermoplasma acidophilumIn: Journal of Molecular Biology (JMB) Vol. 324 (2002) Nr. 5, pp. 1041 - 1050Online Full Text: dx.doi.org/
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The Core Structure of TMC-95A Is a Promising Lead for Reversible Proteasome InhibitionIn: Angewandte Chemie International Edition Vol. 41 (2002) Nr. 5, pp. 780 - 783Online Full Text: dx.doi.org/