ZMB Member Robert Huber

ZMB Member
Robert Huber

Next ZMB-Member
portrait photo of ZMB member Robert Huber
© Axel Griesch, MPI for Biochemistry

Prof. Dr. Dr. h.c. mult. Robert Huber FRS

Group

Structure Research

Center of Medical Biotechnology
Room: S03 S02 A45
University of Duisburg-Essen
45141 Essen
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried

Research Overview

Professional interests

Structure and function of biological macromolecules, in particular those of large complex aggregates.

Systems studied

  • Proteases and their natural and synthetic inhibitors.
  • Metalloenzymes (iron, nickel, molybdenum, copper).
  • Proteins of the immune system (antibodies and antibody receptors).
  • Protein hormones and their receptors.
  • Protein kinases.
  • Proteins of amino acid biosynthesis (PLP containing enzymes).
  • Proteins of cofactor and vitamin biosynthesis.
  • Proteins of energy and electron transport.

Methods development

  • Patterson methods in crystallography.
  • Methods of structure determination of proteins and protein ligand complexes by NMR.
  • Synthesis and use of electron rich metal clusters.
  • Crystal annealing and improvement, methods and instruments.
  • Analysis and evaluation of targets for research and application in pharmacology and crop science.

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Selected Publications

    Journal articles

  • Lee, Jung-Hoon; Bollschweiler, Daniel; Schäfer, Tillman; Huber, Robert
    Structural basis for the regulation of nucleosome recognition and HDAC activity by histone deacetylase assemblies
    In: Science Advances Vol. 7 (2021) Nr. 2, pp. eabd4413
    ISSN: 2375-2548
    Online Full Text: dx.doi.org/ (Open Access)
  • Beaufort, Nathalie; Scharrer, Eva; Kremmer, Elisabeth; Lux, Vanda; Ehrmann, Michael; Huber, Robert; Houlden, Henry H.; Werring, David John; Haffner, Christof; Dichgans, Martin
    Cerebral small vessel disease-related protease HtrA1 processes latent TGF-β binding protein 1 and facilitates TGF-β signaling
    In: Proceedings of the National Academy of Sciences of the United States of America Vol. 111 (2014) Nr. 46, pp. 16496 - 16501
    ISSN: 1091-6490; 0027-8424
    Online Full Text: dx.doi.org/
  • Arciniega, M.; Beck, P.; Lange, O. F.; Groll, M.; Huber, Robert
    Differential global structural changes in the core particle of yeast and mouse proteasome induced by ligand binding
    In: Proceedings of the National Academy of Sciences of the United States of America Vol. 111 (2014) Nr. 26, pp. 9479 - 9484
    ISSN: 1091-6490; 0027-8424
    Online Full Text: dx.doi.org/
  • Lesca, E.; Lammens, A.; Huber, Robert; Augustin, M.
    Structural analysis of the human fibroblast growth factor receptor 4 kinase
    In: Journal of Molecular Biology Vol. 426 (2014) Nr. 22, pp. 3744 - 3756
    ISSN: 1089-8638; 0022-2836
    Online Full Text: dx.doi.org/
  • Baek, Sohee; Kang, Nam Joo; Popowicz, Grzegorz M.; Arciniega, Marcelino; Jung, Sung Keun; Byun, Sanguine; Song, Nu Ry; Heo, Yong-Seok; Kim, Bo Yeon; Lee, Hyong Joo; Holak, Tad A.; Augustin, Martin; Bode, Ann M.; Huber, Robert; Dong, Zigang; Lee, Ki Won
    Structural and Functional Analysis of the Natural JNK1 Inhibitor Quercetagetin
    In: Journal of Molecular Biology Vol. 425 (2013) Nr. 2, pp. 411 - 423
    ISSN: 1089-8638; 0022-2836
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Huber, Robert
    “How I Chose Research on Proteases or, More Correctly, How it Chose Me”
    In: Angewandte Chemie International Edition Vol. 52 (2013) Nr. 1, pp. 68 - 73
    ISSN: 1521-3773
    Online Full Text: dx.doi.org/
  • Weski, Juliane; Meltzer, Michael; Spaan, Lina; Mönig, Timon; Oeljeklaus, Julian; Hauske, Patrick; Vouilleme, Lars; Volkmer, Rudolf; Boisguerin, Prisca; Boyd, Dana; Huber, Robert; Kaiser, Markus; Ehrmann, Michael
    Chemical Biology Approaches Reveal Conserved Features of a C-Terminal Processing PDZ Protease
    In: ChemBioChem Vol. 13 (2012) Nr. 3, pp. 402 - 408
    ISSN: 1439-4227; 1439-7633
    Online Full Text: dx.doi.org/
  • Gallastegui, Nerea; Beck, Philipp; Arciniega, Marcelino; Huber, Robert; Hillebrand, Stefan; Groll, Michael
    Hydroxyureas as noncovalent proteasome inhibitors
    In: Angewandte Chemie International Edition Vol. 51 (2012) Nr. 1, pp. 247 - 249
    ISSN: 1521-3773
    Online Full Text: dx.doi.org/
  • Gallastegui, Nerea; Stein, Martin; Schmidt, Boris; Kloetzel, Peter-Michael; Huber, Robert; Groll, Michael; Gräwert, Melissa Ann
    Elucidation of the α-keto-aldehyde binding mechanism : A lead structure motif for proteasome inhibition
    In: Angewandte Chemie International Edition Vol. 50 (2011) Nr. 2, pp. 542 - 544
    ISSN: 1521-3773
    Online Full Text: dx.doi.org/
  • Clausen, Tim; Kaiser, Markus; Huber, Robert; Ehrmann, Michael
    HTRA proteases : regulated proteolysis in protein quality control
    In: Nature Reviews Molecular Cell Biology Vol. 12 (2011) Nr. 3, pp. 152 - 162
    ISSN: 1471-0072
    Online Full Text: dx.doi.org/
  • Sitar, Tomasz; Gallinger, Julia; Ducka, Anna M.; Ikonen, Teemu P.; Wohlhoefler, Michael; Schmoller, Kurt M.; Bausch, Andreas R.; Joel, Peteranne; Trybus, Kathleen M.; Noegel, Angelika A.; Schleicher, Michael; Huber, Robert; Holak, Tad A.
    Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly
    In: Proceedings of the National Academy of Sciences of the United States of America Vol. 108 (2011) Nr. 49, pp. 19575 - 19580
    ISSN: 1091-6490; 0027-8424
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Merdanovic, Melisa; Clausen, Tim; Kaiser, Markus; Huber, Robert; Ehrmann, Michael
    Protein quality control in the bacterial periplasm
    In: Annual Review of Microbiology Vol. 65 (2011) pp. 149 - 168
    ISSN: 1545-3251; 0066-4227
    Online Full Text: dx.doi.org/
  • Schneider, Elisabeth V.; Böttcher, Jark; Blaesse, Michael; Neumann, Lars; Huber, Robert; Maskos, Klaus
    The structure of CDK8/CycC implicates specificity in the CDK/cyclin family and reveals interaction with a deep pocket binder
    In: Journal of Molecular Biology Vol. 412 (2011) Nr. 2, pp. 251 - 266
    ISSN: 0022-2836; 1089-8638
    Online Full Text: dx.doi.org/
  • Hasenbein, Sonja; Meltzer, Michael; Hauske, Patrick; Kaiser, Markus; Huber, Robert; Clausen, Tim; Ehrmann, Michael
    Conversion of a regulatory into a degradative protease
    In: Journal of Molecular Biology Vol. 397 (2010) Nr. 4, pp. 957 - 966
    ISSN: 0022-2836; 1089-8638
    Online Full Text: dx.doi.org/
  • Merdanovic, Melisa; Mamant, Nicolette; Meltzer, Michael; Poepsel, Simon; Auckenthaler, Alexandra; Melgaard, Rie; Hauske, Patrick; Nagel-Steger, Luitgard; Clarke, Anthony R.; Kaiser, Markus; Huber, Robert; Ehrmann, Michael
    Determinants of structural and functional plasticity of a widely conserved protease chaperone complex
    In: Nature Structural & Molecular Biology Vol. 17 (2010) Nr. 7, pp. 837 - 843
    ISSN: 1545-9993; 1545-9985
    Online Full Text: dx.doi.org/
  • Krojer, Tobias; Sawa, Justyna; Huber, Robert; Clausen, Tim
    HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues
    In: Nature Structural & Molecular Biology Vol. 17 (2010) Nr. 7, pp. 844 - 852
    ISSN: 1545-9993; 1545-9985
    Online Full Text: dx.doi.org/
  • Ottmann, Christian; Rose, Rolf; Huttenlocher, Franziska; Cedzich, Anna; Hauske, Patrick; Kaiser, Markus; Huber, Robert; Schaller, Andreas
    Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3
    In: Proceedings of the National Academy of Sciences of the United States of America Vol. 106 (2009) Nr. 40, pp. 17223 - 17228
    ISSN: 1091-6490; 0027-8424
    Online Full Text: dx.doi.org/
  • Meltzer, Michael; Hasenbein, Sonja; Mamant, Nicolette; Merdanovic, Melisa; Poepsel, Simon; Hauske, Patrick; Kaiser, Markus; Huber, Robert; Krojer, Tobias; Clausen, Tim; Ehrmann, Michael
    Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli
    In: Research in Microbiology Vol. 160 (2009) Nr. 9, pp. 660 - 666
    ISSN: 0923-2508; 1769-7123
    Online Full Text: dx.doi.org/
  • Clerc, Jérôme; Groll, Michael; Illich, Damir J.; Bachmann, André S.; Huber, Robert; Schellenberg, Barbara; Dudler, Robert; Kaiser, Markus
    Synthetic and structural studies on Syringolin A and B reveal critical determinants for selectivity and potency of proteasome inhibition
    In: Proceedings of the National Academy of Sciences of the United States of America Vol. 106 (2009) Nr. 16, pp. 6507 - 6512
    ISSN: 1091-6490; 0027-8424
    Online Full Text
  • Groll, Michael; Schellenberg, Barbara; Bachmann, André S.; Archer, Crystal R.; Huber, Robert; Powell, Tracy K.; Lindow, Steven; Kaiser, Markus; Dudler, Robert
    A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism
    In: Nature Vol. 452 (2008) pp. 755 - 758
    ISSN: 0028-0836; 1476-4687
    Online Full Text
  • Krojer, Tobias; Pangerl, Karen; Kurt, Juliane; Sawa, Justyna; Stingl, Christoph; Mechtler, Karl; Huber, Robert; Ehrmann, Michael; Clausen, Tim
    Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins
    In: Proceedings of the National Academy of Sciences of the United States of America Vol. 22 (2008) pp. 7702 - 7707
    ISSN: 1091-6490; 0027-8424
    Online Full Text
  • Kim, Jeong-Sun; Groll, Michael; Musiol, Hans-Jürgen; Behrendt, Raymond; Kaiser, Markus; Moroder, Luis; Huber, Robert; Brandstetter, Hans
    Navigation inside a protease: Substrate selection and product exit in the tricorn protease from Thermoplasma acidophilum
    In: Journal of Molecular Biology Vol. 324 (2002) Nr. 5, pp. 1041 - 1050
    ISSN: 0022-2836; 1089-8638
    Online Full Text: dx.doi.org/
  • Kaiser, Markus; Groll, Michael; Renner, Christian; Huber, Robert; Moroder, Luis
    The Core Structure of TMC-95A Is a Promising Lead for Reversible Proteasome Inhibition
    In: Angewandte Chemie - International Edition Vol. 41 (2002) Nr. 5, pp. 780 - 783
    ISSN: 0044-8249; 0570-0833; 1433-7851
    Online Full Text
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