Imaging Center Campus Essen

Imaging Center Campus Essen - ICCE

The “Imaging Center Campus Essen” (ICCE) is an imaging core facility of the Center of Medical Biotechnology (ZMB) at the University of Duisburg-Essen. The ICCE provides state-of-the-art equipment for advanced light microscopy techniques, technical advice in sample preparation and support in image analysis for all ZMB researchers and collaborating groups of the University Duisburg-Essen or external universities and research institutions. Please, contact us to discuss if your project can be conducted at our microscope systems.

Highlights and News

CRC1430 funding approved

May 25, 2021

​We are happy to announce that our proposal has been approved by the DFG on Tuesday, May 25th, 2021, and the work in our Collaborative Research Centre 1430 "Molecular Mechanisms of Cell State Transitions" can start now. The ICCE will participate as central service project to support scientists in the detection and modulation of cell state transitions using advanced light microscopy and image analysis.

For more information, please visit the CRC1430 website.

Read more

OMERO for image data management

October 28, 2020

Omero Logo
For the management of microscopy data, the ICCE operates a dedicated OMERO instance in cooperation with the Research Data Services (RDS), the eScience unit of ZIM and the CVIS. More information about OMERO can be found here.

Please contact us, if you are interested in using OMERO.

Re-Opening of ICCE

May 11, 2020

Under consideration of special safety measures, instructed users are allowed to use the microscope systems of the ICCE again from May 11, 2020. To keep the risk of infection as low as possible, the recommendations of GerBI-GMB will apply for use of microscopes. That means:

  • Only one person may be present in a microscope room at a time.
  • All parts of the microscope and computer that are touched during a session (e.g. eyepiece, touchpad, joystick, focus adjustment knobs, PC keyboard and mouse) must be covered with the provided cling film (“Frischhaltefolie”). Remove the wrap after finishing your session and dispose it in the provided autoclave bags. (for details check photos in GerBI-GMB recommendations or contact us)
  • Clean surfaces with the provided surface disinfectant after usage.
  • The wearing of a mouth and nose protection mask and (own!) safety goggles is optional.

At present, the ICCE cannot provide training for new users. We are working on possibilities to offer this again soon. Currently, experiments on the microscopes may only be performed by trained users. In cooperation with us, we will find a possibility to measure samples of new users in urgent cases. Please contact us for this purpose.

Stay healthy!

Coronavirus Shutdown of ICCE

March 23, 2020

Following the recommendations of the University Board (https://www.uni-due.de/de/covid-19/index-en.php) to maintain only essential services, we decided to close ICCE from now until further notice. Initially, we will delete all bookings for the next 14 days. This may be extended depending on how the situation develops.

We will be available via Email and phone if you need help in image processing and analysis or future experimental planning.

We will keep you informed and hope to get back to normal operation soon.

Stay healthy!

Presentation of Zeiss AxioLab5

March 2020
The company Zeiss will present their microscope AxioLab5 as a tool for a digital classroom. The presentation will take place on 03/03/2020 at 10 a.m. in S05 V05 F55. Guests are welcome.

CIC-RDM meeting in Essen

February 2020
We will host the CIC-RDM Meeting on 02/03 to 02/04. The topics will be to develop workflows/usecases of OMERO, funding opportunities for RDM, data policies for core facilities and training concepts for RDM software.

If you are interested to join, please contact us.

Our office moved (again)

October 2019
We had to move our office. From now on you will find us in S05 V05 F48.

Zeiss Axio Observer moved (again)

August 2019
We have to move the Zeiss Axio Observer from the room T03 R02 D93 in the room  S05 V-01 E98. It will be functional again at beginning of September. Please contact the ICCE if you need access to the new room.

Zeiss Axio Observer moved

July 2019
We had to move the Zeiss Axio Observer from the room T03 R02 D03 in the room T03 R02 D93 (right across the hallway). It will be functional in the first week of August.

New office in T03 R01 D61

July 2019
We had to move our office. From now on you will find us in T03 R01 D61.

Invitation to a talk given by Leica Microsystems

August 2018
Heike Glauner (Leica Microsystems, Application Workflow Specialist EMEA Confocal Microscopy/ FLIM & FCS) will give a talk on: "Your New Leica TCS SP8X: Looking Forward to Your Discoveries"

Tuesday, August 14th, 1 pm in room S05 V01 E69

Abstract:

The newly installed Leica TCS SP8X confocal expands your possibilities in imaging in several dimensions. This talk aims to give you an overview about the techniques that can be applied by the system, such as Fluorescence Lifetime Imaging Microscopy (FLIM), Fluorescence Correlation Spectroscopy (FCS), the Lightning detection concept to extend the resolution down to 120 nm, the Resonant Scanner for fast imaging, or the new Navigator tool for fast overviews of your samples and the identification of important details in an instant. With the White Light Laser as excitation source and the Leica spectral detector you will have maximum spectral freedom. A Rapp OptoElectronic laser ablation system completes the configuration so that all in all a broad range of applications becomes possible. Come and find out what the Leica TCS SP8X can do for your research work.

New Microscope at ICCE: Leica TCS SP8X Falcon

August 2018

We are excited to announce that our new confocal microscope system Leica TCS SP8X Falcon for Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) was successfully installed at the ICCE. Additional special features beyond FLIM and FC(C)S are the White Light Laser (WLL) as excitation source in combination with Leica´s spectral detection system for maximal spectral freedom, Lightning (Leica´s Detection and Image Information Extraction add-on for “confocal super-resolution” down to 120 nm), Navigator and HCS A for fast overviews, automated image acquisition and screening, a Resonant Scanner for fast imaging and a UVA (355 nm) laser ablation system (Rapp OptoElectronic) for laser microirradiation (e.g. DNA damage) and cutting/ablation. You can find more detailed information about the technical specifications under equipment.

The new equipment was obtained through DFG funding (Major Research Instrumentation Programme as per Art. 91b GG, INST 20876/294-1 FUGG) to Prof. Shirley Knauer and other ZMB members and will be fully integrated into the ICCE. A special thanks to all those involved.

New Coworker (academic part-time) at ICCE

April 2018
We have a new co-worker at the ICCE: Dr. Johannes Koch will be available in part-time (Mondays, 1–6 pm and Fridays, 9–12 am) and will be mainly responsible for measurements within third-party funded projects like the CRC1093.

Two new Microscopes at ICCE: DeltaVision Elite (GE Healthcare) and Nikon Eclipse Ti2 TIRF DualCam

December 2017
We are excited to announce that there are two new microscope systems available at the ICCE:

The DeltaVision Elite (GE Healthcare) is a high-resolution widefield deconvolution fluorescence microscope optimized for (fast and/or long-term) live cell imaging. The integrated image restoration tool (deconvolution) uses knowledge of the 3D imaging properties of the microscope (point spread function) to deblur acquired microscopy data by remapping the out-of-focus signal, thereby significantly enhancing image resolution and contrast.

The Nikon Eclipse Ti2 TIRF DualCam is a widefield fluorescence microscope with fully motorized 4-color TIRF illumination, two-camera setup for simultaneous dual color imaging and FRAP module for photobleaching or photoactivation/conversion experiments. Due to high-power lasers and ultra-sensitive cameras this system can also be used for imaging single molecules dynamics.

You can find more detailed information about the technical specifications under equipment.

Both instruments were obtained through DFG funding (Major Research Instrumentation Programme as per Art. 91b GG, INST 20876/274-1 and 20876/275-1 FUGG) to Prof. Stefan Westermann and will be fully integrated into the ICCE. A special thanks to all those involved.

New Publication and Cover Image in The EMBO Journal

January 2017
Clearance of ruptured lysosomes by autophagy (lysophagy) is driven by the novel ELDR (endolysosomal damage response) complex and involves deubiquitination and p97 activity. For more infomation see

Cover-embo-journal-volume-36-issue-2-01-17
Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H. VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy. EMBO J. 2017 Jan 17;36(2):135-150.

Team

Nina-schulze

Dr. Nina Schulze

ICCE Manager

nina.schulze@uni-due.de

Johannes Koch

Dr. Johannes Koch

Research Associate
project-related support

johannes.koch@uni-due.de

Contact

Phone: +49201 183 2622

Room: S05 V05 F48

Address:
Campus Essen
Universitätsstraße 2
45141 Essen



Bild Johannes homepage

Dr. Johannes Koch

Phone: +49201 183 2622

Room: S05 V05 F48

Address:
Campus Essen
Universitätsstraße 2
45141 Essen

Johannes.Koch@uni-due.de

Latest Publications

Kamps, D., Koch, J., Juma, VO., Campillo-Funollet, E., Graessl, M., Banerjee, S., Mazel, T., Chen, X., Wu, YW., Portet, S., Madzvamuse, A., Nalbant, P., Dehmelt, L. (2020). Optogenetic tuning reveals Rho amplification-dependent dynamics of a cell contraction signal networkCell Rep 33(9).

Kornakov, N., Mollers, B., and Westermann, S. (2020). The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation. J Cell Biol 219.

Kollenda, S., Kopp, M., Wens, J., Koch, J., Schulze, N., Papadopoulos, C., Pohler, R., Meyer, H., and Epple, M. (2020).
A pH-sensitive fluorescent protein sensor to follow the pathway of calcium phosphate nanoparticles into cells. Acta Biomater 111, 406-417.

>> More publications