Imaging Centre Campus Essen
Imaging Centre Campus Essen - ICCE
The “Imaging Centre Campus Essen” (ICCE) is an imaging core facility of the Centre of Medical Biotechnology (ZMB) at the University of Duisburg-Essen. The ICCE provides state-of-the-art equipment for advanced light microscopy techniques, technical advice in sample preparation and support in image analysis for all ZMB researchers and collaborating groups of the University Duisburg-Essen or external universities and research institutions. Please, contact us to discuss if your project can be conducted at our microscope systems.
Highlights and News
Invitation to a talk given by Leica Microsystems
Heike Glauner (Leica Microsystems, Application Workflow Specialist EMEA Confocal Microscopy/ FLIM & FCS) will give a talk on: "Your New Leica TCS SP8X: Looking Forward to Your Discoveries"
Tuesday, August 14th, 1 pm in room S05 V01 E69
The newly installed Leica TCS SP8X confocal expands your possibilities in imaging in several dimensions. This talk aims to give you an overview about the techniques that can be applied by the system, such as Fluorescence Lifetime Imaging Microscopy (FLIM), Fluorescence Correlation Spectroscopy (FCS), the Lightning detection concept to extend the resolution down to 120 nm, the Resonant Scanner for fast imaging, or the new Navigator tool for fast overviews of your samples and the identification of important details in an instant. With the White Light Laser as excitation source and the Leica spectral detector you will have maximum spectral freedom. A Rapp OptoElectronic laser ablation system completes the configuration so that all in all a broad range of applications becomes possible. Come and find out what the Leica TCS SP8X can do for your research work.
New Microscope at ICCE: Leica TCS SP8X Falcon
We are excited to announce that our new confocal microscope system Leica TCS SP8X Falcon for Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) was successfully installed at the ICCE. Additional special features beyond FLIM and FC(C)S are the White Light Laser (WLL) as excitation source in combination with Leica´s spectral detection system for maximal spectral freedom, Lightning (Leica´s Detection and Image Information Extraction add-on for “confocal super-resolution” down to 120 nm), Navigator and HCS A for fast overviews, automated image acquisition and screening, a Resonant Scanner for fast imaging and a UVA (355 nm) laser ablation system (Rapp OptoElectronic) for laser microirradiation (e.g. DNA damage) and cutting/ablation. You can find more detailed information about the technical specifications under equipment.
The new equipment was obtained through DFG funding (Major Research Instrumentation Programme as per Art. 91b GG, INST 20876/294-1 FUGG) to Prof. Shirley Knauer and other ZMB members and will be fully integrated into the ICCE. A special thanks to all those involved.
New Coworker (academic part-time) at ICCE
We have a new co-worker at the ICCE: Dr. Johannes Koch will be available in part-time (Mondays, 1–6 pm and Fridays, 9–12 am) and will be mainly responsible for measurements within third-party funded projects like the CRC1093.
Two new Microscopes at ICCE: DeltaVision Elite (GE Healthcare) and Nikon Eclipse Ti2 TIRF DualCam
We are excited to announce that there are two new microscope systems available at the ICCE:
The DeltaVision Elite (GE Healthcare) is a high-resolution widefield deconvolution fluorescence microscope optimized for (fast and/or long-term) live cell imaging. The integrated image restoration tool (deconvolution) uses knowledge of the 3D imaging properties of the microscope (point spread function) to deblur acquired microscopy data by remapping the out-of-focus signal, thereby significantly enhancing image resolution and contrast.
The Nikon Eclipse Ti2 TIRF DualCam is a widefield fluorescence microscope with fully motorized 4-color TIRF illumination, two-camera setup for simultaneous dual color imaging and FRAP module for photobleaching or photoactivation/conversion experiments. Due to high-power lasers and ultra-sensitive cameras this system can also be used for imaging single molecules dynamics.
You can find more detailed information about the technical specifications under equipment.
Both instruments were obtained through DFG funding (Major Research Instrumentation Programme as per Art. 91b GG, INST 20876/274-1 and 20876/275-1 FUGG) to Prof. Stefan Westermann and will be fully integrated into the ICCE. A special thanks to all those involved.
New Publication and Cover Image in The EMBO Journal
Clearance of ruptured lysosomes by autophagy (lysophagy) is driven by the novel ELDR (endolysosomal damage response) complex and involves deubiquitination and p97 activity. For more infomation see
Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M
, Weihl CC, Meyer H
. VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy
. EMBO J.
2017 Jan 17;36(2):135-150.