Imaging Center Campus Essen
Imaging Center Campus Essen - ICCE
The “Imaging Center Campus Essen” (ICCE) is an imaging core facility of the Center of Medical Biotechnology (ZMB) at the University of Duisburg-Essen. The ICCE provides state-of-the-art equipment for advanced light microscopy techniques, technical advice in sample preparation and support in image analysis for all ZMB researchers and collaborating groups of the University Duisburg-Essen or external universities and research institutions. Please, contact us to discuss if your project can be conducted at our microscope systems.
Highlights and News
OMERO for image data management
October 28, 2020
For the management of microscopy data, the ICCE operates a dedicated OMERO instance in cooperation with the Research Data Services (RDS), the eScience unit of ZIM and the CVIS. More information about OMERO can be found here.
Please contact us, if you are interested in using OMERO.
Re-Opening of ICCE
May 11, 2020
Under consideration of special safety measures, instructed users are allowed to use the microscope systems of the ICCE again from May 11, 2020. To keep the risk of infection as low as possible, the recommendations of GerBI-GMB will apply for use of microscopes. That means:
- Only one person may be present in a microscope room at a time.
- All parts of the microscope and computer that are touched during a session (e.g. eyepiece, touchpad, joystick, focus adjustment knobs, PC keyboard and mouse) must be covered with the provided cling film (“Frischhaltefolie”). Remove the wrap after finishing your session and dispose it in the provided autoclave bags. (for details check photos in GerBI-GMB recommendations or contact us)
- Clean surfaces with the provided surface disinfectant after usage.
- The wearing of a mouth and nose protection mask and (own!) safety goggles is optional.
At present, the ICCE cannot provide training for new users. We are working on possibilities to offer this again soon. Currently, experiments on the microscopes may only be performed by trained users. In cooperation with us, we will find a possibility to measure samples of new users in urgent cases. Please contact us for this purpose.
Coronavirus Shutdown of ICCE
March 23, 2020
Following the recommendations of the University Board (https://www.uni-due.de/de/covid-19/index-en.php) to maintain only essential services, we decided to close ICCE from now until further notice. Initially, we will delete all bookings for the next 14 days. This may be extended depending on how the situation develops.
We will be available via Email and phone if you need help in image processing and analysis or future experimental planning.
We will keep you informed and hope to get back to normal operation soon.
Presentation of Zeiss AxioLab5
The company Zeiss will present their microscope AxioLab5 as a tool for a digital classroom. The presentation will take place on 03/03/2020 at 10 a.m. in S05 V05 F55. Guests are welcome.
CIC-RDM meeting in Essen
We will host the CIC-RDM Meeting on 02/03 to 02/04. The topics will be to develop workflows/usecases of OMERO, funding opportunities for RDM, data policies for core facilities and training concepts for RDM software.
If you are interested to join, please contact us.
Our office moved (again)
We had to move our office. From now on you will find us in S05 V05 F48.
Zeiss Axio Observer moved (again)
We have to move the Zeiss Axio Observer from the room T03 R02 D93 in the room S05 V-01 E98. It will be functional again at beginning of September. Please contact the ICCE if you need access to the new room.
Zeiss Axio Observer moved
We had to move the Zeiss Axio Observer from the room T03 R02 D03 in the room T03 R02 D93 (right across the hallway). It will be functional in the first week of August.
New office in T03 R01 D61
We had to move our office. From now on you will find us in T03 R01 D61.
Invitation to a talk given by Leica Microsystems
Heike Glauner (Leica Microsystems, Application Workflow Specialist EMEA Confocal Microscopy/ FLIM & FCS) will give a talk on: "Your New Leica TCS SP8X: Looking Forward to Your Discoveries"
Tuesday, August 14th, 1 pm in room S05 V01 E69
The newly installed Leica TCS SP8X confocal expands your possibilities in imaging in several dimensions. This talk aims to give you an overview about the techniques that can be applied by the system, such as Fluorescence Lifetime Imaging Microscopy (FLIM), Fluorescence Correlation Spectroscopy (FCS), the Lightning detection concept to extend the resolution down to 120 nm, the Resonant Scanner for fast imaging, or the new Navigator tool for fast overviews of your samples and the identification of important details in an instant. With the White Light Laser as excitation source and the Leica spectral detector you will have maximum spectral freedom. A Rapp OptoElectronic laser ablation system completes the configuration so that all in all a broad range of applications becomes possible. Come and find out what the Leica TCS SP8X can do for your research work.
New Microscope at ICCE: Leica TCS SP8X Falcon
We are excited to announce that our new confocal microscope system Leica TCS SP8X Falcon for Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) was successfully installed at the ICCE. Additional special features beyond FLIM and FC(C)S are the White Light Laser (WLL) as excitation source in combination with Leica´s spectral detection system for maximal spectral freedom, Lightning (Leica´s Detection and Image Information Extraction add-on for “confocal super-resolution” down to 120 nm), Navigator and HCS A for fast overviews, automated image acquisition and screening, a Resonant Scanner for fast imaging and a UVA (355 nm) laser ablation system (Rapp OptoElectronic) for laser microirradiation (e.g. DNA damage) and cutting/ablation. You can find more detailed information about the technical specifications under equipment.
The new equipment was obtained through DFG funding (Major Research Instrumentation Programme as per Art. 91b GG, INST 20876/294-1 FUGG) to Prof. Shirley Knauer and other ZMB members and will be fully integrated into the ICCE. A special thanks to all those involved.
New Coworker (academic part-time) at ICCE
We have a new co-worker at the ICCE: Dr. Johannes Koch will be available in part-time (Mondays, 1–6 pm and Fridays, 9–12 am) and will be mainly responsible for measurements within third-party funded projects like the CRC1093.
Two new Microscopes at ICCE: DeltaVision Elite (GE Healthcare) and Nikon Eclipse Ti2 TIRF DualCam
We are excited to announce that there are two new microscope systems available at the ICCE:
The DeltaVision Elite (GE Healthcare) is a high-resolution widefield deconvolution fluorescence microscope optimized for (fast and/or long-term) live cell imaging. The integrated image restoration tool (deconvolution) uses knowledge of the 3D imaging properties of the microscope (point spread function) to deblur acquired microscopy data by remapping the out-of-focus signal, thereby significantly enhancing image resolution and contrast.
The Nikon Eclipse Ti2 TIRF DualCam is a widefield fluorescence microscope with fully motorized 4-color TIRF illumination, two-camera setup for simultaneous dual color imaging and FRAP module for photobleaching or photoactivation/conversion experiments. Due to high-power lasers and ultra-sensitive cameras this system can also be used for imaging single molecules dynamics.
You can find more detailed information about the technical specifications under equipment.
Both instruments were obtained through DFG funding (Major Research Instrumentation Programme as per Art. 91b GG, INST 20876/274-1 and 20876/275-1 FUGG) to Prof. Stefan Westermann and will be fully integrated into the ICCE. A special thanks to all those involved.
New Publication and Cover Image in The EMBO Journal
Clearance of ruptured lysosomes by autophagy (lysophagy) is driven by the novel ELDR (endolysosomal damage response) complex and involves deubiquitination and p97 activity. For more infomation see