The expression dynaklim is a shortcut for „dynamic adaptation of regional planning and developmental processes to the impact of climate change in the Emscher-Lippe region (Ruhrgebiet)”.
Background of the project is the expected climate change in the Emscher-Lippe region which is characterized by mild wet winters and hot dry summers interrupted by torrential rainfalls. Focuses of the dynaklim project are, amongst others, prognosticated climate changes and their influences on water quality, water availability and the utilization of water in this region. Based on a collaboration of various partners in this project (over 40 network partners from fields of politics, science, economy, civil initiatives, etc.) a roadmap 2020 will be developed, describing an adaptation strategy according to the predicted climate change. dynaklim can therefore be seen as a national and international path breaking model project for climate adaptation of densely populated areas. The task of the Biofilm Centre is to perform research on the influence of temperature on hygienically relevant microorganisms (Pseudomonas aeruginosa, Legionella pneumophila, Escherichia coli and Klebsiella pneumonia) in drinking water distribution systems in close collaboration with the IWW Water Centre (Mülheim an der Ruhr). It is known that there are threshold temperatures, above which growth of hygienically relevant bacteria starts and, with increasing temperatures, can be enhanced. In laboratory experiments the integration of the bacteria mentioned above into drinking water biofilms at different temperatures (8 °C - 29 °C) will be examined. The persistence of the target organisms in the biofilms will be monitored, using both conventional culture methods and culture-independent fluorescence in situ hybridzation (FISH). The application of the FISH method will additionally give the information if the target organisms enter a viable but nonculturable (VBNC) state. Population analysis of the biofilms will be done with denaturing gradient gel electrophoresis (DGGE). In addition to the laboratory experiments, field work will be performed. Three different materials (EPDM, PE80/100, and stainless steel) are exposed in a public drinking water distribution system. Sampling of the materials will be done seasonally (winter, spring, summer) after 4 months of exposure and biofilm formation as well as the presence of hygienically relevant microorganisms will be examined.
Fig. 1: Biofilmreactor in field experiments
Fig. 2: Rotary piston reactor for laboratory experiments