ZMB Member Manuela Wülling

ZMB Member
Manuela Wülling

Next ZMB-Member
portrait photo of ZMB member Manuela Wülling

Dr. Manuela Wülling

Group

Developmental Biology

Transcriptional and epigenetic control of endochondral ossification

Center of Medical Biotechnology (ZMB)
Faculty of Biology
University of Duisburg-Essen
Universitätsstr. 2
45141 Essen

The main interest of the group are the transcriptional mechanisms that govern chondrocyte development and pathology. Dysregulation of chondrocyte proliferation and differentiation lead to shortened and malformed endochondral bones and the degenerative diseases of the connecting joints. Our goal is to decipher how transcriptional cascades interact to coordinate cell cycle progression and differentiation of skeletal cells. Epigenetic modifiers link chromatin structure to the transcriptional output. Changes in epigenetic profiles have been reported to affect pathological processes like osteoarthritis. Using next generation sequencing technologies, we are currently integrating epigenetic and transcriptional profiles to understand how alterations in chromatin structure and transcription factor binding affect skeletal development and disease.

Publications

    Journal articles

  • Neu, Christoph; Wülling, Manuela; Waterkamp, Christoph; Hoffmann, Daniel; Vortkamp, Andrea
    Bath : A Bayesian approach to analyze epigenetic transitions reveals a dual role of H3K27me3 in chondrogenesis
    In: Epigenetics and Chromatin, Vol. 18, 2025, Nr. 1, 38
    DOI (Open Access)
  • Wülling, Manuela; Neu, Christoph; Thiesen, Andrea M.; Kitanovski, Simo; Cao, Yingying; Lange, Anja; Westendorf, Astrid; Hoffmann, Daniel; Vortkamp, Andrea
    Epigenetic mechanisms mediating cell state transitions in chondrocytes
    In: Journal of Bone and Mineral Research (JBMR), Vol. 36, 2021, Nr. 5, pp. 968 – 985
    DOI, Online Full Text (Open Access)
  • Wülling, Manuela; Schneider, Sabine; Schröther, Verena A.; Waterkamp, Christoph; Hoffmann, Daniel; Vortkamp, Andrea
    Wnt5a is a transcriptional target of Gli3 and Trps1 at the onset of chondrocyte hypertrophy
    In: Developmental Biology, Vol. 457, 2020, Nr. 1, pp. 104 – 118
    DOI (Open Access)
  • Grüneboom, Anika; Hawwari, Ibrahim; Weidner, Daniela; Culemann, Stephan; Müller, Sylvia; Henneberg, Sophie; Brenzel, Alexandra; Merz, Simon; Bornemann, Lea; Zec, Kristina; Wülling, Manuela; Kling, Lasse; Hasenberg, Mike; Voortmann, Sylvia; Lang, Stefanie; Baum, Wolfgang; Ohs, Alexandra; Kraff, Oliver; Quick, Harald H.; Jäger, Marcus; Landgraeber, Stefan; Dudda, Marcel; Danuser, Renzo; Stein, Jens V.; Rohde, Manfred; Gelse, Kolja; Garbe, Annette I.; Adamczyk, Alexandra; Westendorf, Astrid; Hoffmann, Daniel; Christiansen, Silke; Engel, Daniel Robert; Vortkamp, Andrea; Krönke, Gerhard; Herrmann, Martin; Kamradt, Thomas; Schett, Georg; Hasenberg, Anja; Gunzer, Matthias
    A network of trans-cortical capillaries as mainstay for blood circulation in long bones
    In: Nature Metabolism, Vol. 1, 2019, Nr. 2, pp. 236 – 250
    DOI, Online Full Text (Open Access)
  • Wülling, Manuela; Vortkamp, Andrea
    A newly discovered stem cell that keeps bones growing
    In: Nature, Vol. 567, 2019, Nr. 7747, pp. 178 – 179
    DOI
  • Schröder, Nadine; Wülling, Manuela; Hoffmann, Daniel; Brand-Saberi, Beate; Vortkamp, Andrea
    Atoh8 acts as a regulator of chondrocyte proliferation and differentiation in endochondral bones
    In: PLoS ONE, Vol. 14, 2019, Nr. 8, pp. e0218230
    DOI, Online Full Text (Open Access)
  • Piombo, Virginia; Jochmann, Katja; Hoffmann, Daniel; Wülling, Manuela; Vortkamp, Andrea
    Signaling systems affecting the severity of multiple osteochondromas
    In: Bone: Official Journal of the International Bone and Mineral Society (IBMS), Vol. 111, 2018, pp. 71 – 81
    DOI (Open Access)
  • Wülling, Manuela; Thiesen, Andrea M.; Vortkamp, Andrea
    Epigenetic regulation of chondrocyte differentiation
    18th International Congress of Developmental Biology, 18-22 June, Singapore,
    In: Mechanisms of Development, Vol. 145, 2017, Nr. Supplement: 18th International Congress of Developmental Biology 18-22 June, University Cultural Centre, National University of Singapore, pp. S141
    DOI
  • Kipkeew, Friederike; Kirsch, Manuela; Klein, Diana; Wülling, Manuela; Winterhager, Elke; Gellhaus, Alexandra
    CCN1 (CYR61) and CCN3 (NOV) signaling drives human trophoblast cells into senescence and stimulates migration properties
    In: Cell Adhesion & Migration, Vol. 10, 2016, Nr. 1-2, pp. 163 – 178
    DOI, Online Full Text (Open Access)
  • Kirsch, Michael; Kipkeew, Friederike; Klein, Diana; Wülling, Manuela; Winterhager, Elke; Gellhaus, Alexandra
    CCN3 signaling is dysregulated in the pregnancy disorder preeclampsia
    In: Journal of Reproductive Immunology, Vol. 115, 2016, pp. 71 – 71
    DOI
  • Mattheij, Nadine J. A.; Kunzelmann, Karl; Vortkamp, Andrea; Schreiber, Rainer; Köhler, Ralf; Wülling, Manuela; Nieswandt, Bernhard; Pircher, Joachim; Castoldi, Elisabetta; van Kruchten, Roger; Braun, Attila
    Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage
    In: The FASEB Journal, Vol. 30, 2016, Nr. 2, pp. 727 – 737
    DOI
  • Ousingsawat, Jiraporn; Wanitchakool, Podchanart; Schreiber, Rainer; Wülling, Manuela; Vortkamp, Andrea; Kunzelmann, Karl
    Anoctamin-6 Controls Bone Mineralization by Activating the Calcium Transporter NCX1
    In: The Journal of Biological Chemistry (JBC), Vol. 290, 2015, Nr. 10, pp. 6270 – 6280
    DOI (Open Access)
  • Kipkeew, Friederike; Klein, Diana; Wülling, Manuela; Whitley, Guy; Köninger, Angela; Winterhager, Elke; Gellhaus, Alexandra
    CCN3 (NOV) : A key player in trophoblast differentiation promoting cell cycle exit and migration of extravillous trophoblast cells and its role in preeclampsia
    In: Placenta, Vol. 35, 2014, Nr. 9, pp. A108
    DOI
  • Dittmann, Kai; Wülling, Manuela; Uhmann, Anja; Dullin, Christian; Hahn, Heidi; Schweyer, Stefan; Vortkamp, Andrea; Wienands, Jürgen
    Inactivation of Patched1 in murine chondrocytes causes spinal fusion without inflammation
    In: Arthritis & Rheumatology, Vol. 66, 2014, Nr. 4, pp. 831 – 840
    DOI
  • Ehlen, Harald W. A.; Chinenkova, Milana; Moser, Markus; Munter, Hans-Markus; Krause, Yvonne Stefanie; Gross, Stefanie; Brachvogel, Bent; Wülling, Manuela; Kornak, Uwe; Vortkamp, Andrea
    Inactivation of anoctamin-6/Tmem16f, a regulator of phosphatidylserine scrambling in osteoblasts, leads to decreased mineral deposition in skeletal tissues
    In: Journal of Bone and Mineral Research (JBMR), Vol. 28, 2013, Nr. 2, pp. 246 – 259
    DOI
  • Wülling, Manuela; Pasdziernik, Markus; Moll, Carina; Thiesen, Andrea; Schneider, Sabine; Johannes, Christian; Vortkamp, Andrea
    The multi zinc-finger protein Trps1 acts as a regulator of histone deacetylation during mitosis
    In: Cell Cycle, Vol. 12, 2013, Nr. 14, pp. 2219 – 2232
    DOI
  • Gross, Stefanie; Krause, Yvonne Stefanie; Wülling, Manuela; Vortkamp, Andrea
    Hoxa11 and Hoxd11 regulate chondrocyte differentiation upstream of Runx2 and Shox2 in mice
    In: PLoS ONE, Vol. 7, 2012, Nr. 8, pp. e43553
    DOI, Online Full Text (Open Access)
  • Galli, Giorgio Giacomo; Honnens de Lichtenberg, Kristian; Carrara, Matteo; Hans, Wolfgang; Wülling, Manuela; Mentz, Bettina; Multhaupt, Hinke Arnolda; Fog, Cathrine Kolster; Jensen, Klaus Thorleif; Rappsilber, Juri; Vortkamp, Andrea; Coulton, Les; Fuchs, Helmut; Gailus-Durner, Valérie; Hrabě de Angelis, Martin; Calogero, Raffaele Adolfo; Couchman, John Robert; Lund, Anders Henrik
    Prdm5 Regulates Collagen Gene Transcription by Association with RNA Polymerase II in Developing Bone
    In: PLoS Genetics, Vol. 8, 2012, Nr. 5, pp. e1002711
    DOI (Open Access)
  • Friedrichs, Melanie; Wirsdöerfer, Florian; Flohe, Stefanie; Schneider, Sabine; Wülling, Manuela; Vortkamp, Andrea
    BMP signaling balances proliferation and differentiation of muscle satellite cell descendants.
    In: BMC Cell Biology, Vol. 12, 2011, 26
    DOI, Online Full Text (Open Access)
  • Detsch, Rainer; Hagmeyer, Daniel; Neumann, Markus C; Schaefer, Susannne; Vortkamp, Andrea; Wülling, Manuela; Ziegler, Günter; Epple, Matthias
    The resorption of nanocrystalline calcium phosphates by osteoclast-like cells
    In: Acta Biomaterialia, Vol. 6, 2010, Nr. 8, pp. 3223 – 3233
    DOI
  • Wülling, Manuela; Vortkamp, Andrea
    Transcriptional networks controlling chondrocyte proliferation and differentiation during endochondral ossification.
    In: Pediatric Nephrology, Vol. 25, 2010, Nr. 4, pp. 625 – 631
    DOI
  • Wülling, Manuela; Kaiser, Frank J.; Buelens, Laetitia; Braunholz, Diana; Shivdasani, Ramesh A.; Depping, Reinhard; Vortkamp, Andrea
    Trps1, a regulator of chondrocyte proliferation and differentiation, interacts with the activator form of Gli3
    In: Developmental Biology, Vol. 328, 2009, Nr. 1, pp. 40 – 53
    DOI
  • Kantaputra, P.; Miletich, I.; Lüdecke, Hermann-Josef; Suzuki, E. Y.; Praphanphoj, V.; Shivdasani, R.; Wülling, Manuela; Vortkamp, Andrea; Napierala, D.; Sharpe, P. T.
    Tricho-rhino-phalangeal syndrome with supernumerary teeth
    In: Journal of Dental Research: JDR Online, Vol. 87, 2008, Nr. 11, pp. 1027 – 1031
    DOI
  • Tagariello, A.; Luther, J.; Streiter, M.; Didt-Koziel, L.; Wülling, Manuela; Surmann-Schmitt, C.; Stock, M.; Adam, N.; Vortkamp, Andrea; Winterpacht, A.
    Ucma - A novel secreted factor represents a highly specific marker for distal chondrocytes
    In: Matrix Biology, Vol. 27, 2008, Nr. 1, pp. 3 – 11
    DOI
  • Didt-Koziel, L.; Wülling, Manuela; Vortkamp, Andrea
    The role of growth factors in chondrogenesis and osteogenesis
    In: Current Opinion in Orthopaedics, Vol. 17, 2006, pp. 405 – 411
    DOI
  • Didt-Koziel, L.; Wülling, Manuela; Schneider, Sabine; Vortkamp, Andrea
    Gli3 has repressing and activating functions downstream of Ihh in regulating two distinct steps of chondrocyte differentiation
    In: Development / Company of Biologists, Vol. 132, 2005, Nr. 23, pp. 5249 – 5260
    DOI
  • Wülling, Manuela; Vortkamp, Andrea
    Model systems for human muscoskeletal diseases
    In: Drug Discovery Today: Disease Models, Vol. 1, 2004, Nr. 3, pp. 359 – 364
    DOI