ZMB Member Barbara Saccà

Barbara Saccà

Prof. Dr. Barbara Saccà

Phone: +49 201 183 3395
Email

 
Fakultät für Biologie
Room T03 R01 D36
Universität Duisburg-Essen
45117 Essen

Research Overview

We use DNA nanotechnology to construct simplified models of complex biological systems, where single structural and functional parameters can be manipulated in a predictable fashion.
Our scientific ambition is to gain a better understanding of three fundamental aspects of natural self-assembling systems: hierarchical order, spatial confinement, and dynamic response.

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    Journal articles

  • Jaekel, Andreas; Stegemann, Pierre; Saccà, Barbara
    Manipulating Enzymes Properties with DNA Nanostructures.
    In: Molecules: A Journal of Synthetic Chemistry and Natural Product Chemistry Vol. 24 (2019) Nr. 20, pp. 3694
    ISSN: 1420-3049
  • Kosinski, Richard; Mukhortava, Ann; Pfeifer, Wolfgang; Candelli, Andrea; Rauch, Philipp; Sacca, Barbara
    Sites of high local frustration in DNA origami
    In: Nature Communications Vol. 10 (2019) Nr. 1, pp. 1061
    ISSN: 2041-1723
  • Pfeifer, Wolfgang; Lill, Pascal; Gatsogiannis, Christos; Sacca, Barbara
    Hierarchical Assembly of DNA Filaments with Designer Elastic Properties
    In: ACS Nano Vol. 12 (2018) Nr. 1, pp. 44 - 55
    ISSN: 1936-086X; 1936-0851
  • Pfeifer, Wolfgang; Sacca, Barbara
    Synthetic DNA filaments : from design to applications
    In: Biological Chemistry Vol. 399 (2018) Nr. 7, pp. 773 - 785
    ISSN: 1437-4315; 1431-6730
  • Daems, Devin; Pfeifer, Wolfgang; Rutten, Iene; Sacca, Barbara; Spasic, Dragana; Lammertyn, Jeroen
    Three-Dimensional DNA Origami as Programmable Anchoring Points for Bioreceptors in Fiber Optic Surface Plasmon Resonance Biosensing
    In: ACS Applied Materials & Interfaces Vol. 10 (2018) Nr. 28, pp. 23539 - 23547
    ISSN: 1944-8252; 1944-8244
  • Grossi, Guido; Jaekel, Andreas; Andersen, Ebbe Sloth; Sacca, Barbara
    Enzyme-functionalized DNA nanostructures as tools for organizing and controlling enzymatic reactions
    In: MRS Bulletin Vol. 42 (2017) Nr. 12, pp. 920 - 924
    ISSN: 1938-1425
  • Preisler, Zdeněk; Sacca, Barbara; Whitelam, Stephen
    Irregular model DNA particles self-assemble into a regular structure
    In: Soft Matter Vol. 13 (2017) Nr. 47, pp. 8894 - 8902
    ISSN: 1744-6848; 1744-683X
  • Sprengel, Andreas; Lill, Pascal; Stegemann, Pierre; Bravo-Rodriguez, Kenny; Schöneweiß, Elisa-Charlott; Merdanovic, Melisa; Gudnason, Daniel; Aznauryan, Mikayel; Gamrad; Barcikowski, Stephan; Sanchez-Garcia, Elsa; Birkedal, Victoria; Gatsogiannis, Christos; Ehrmann, Michael; Sacca, Barbara
    Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions
    In: Nature Communications Vol. 8 (2017) pp. 14472
    ISSN: 2041-1723
  • Schöneweiß, Elisa-Charlott; Sacca, Barbara
    The collective behavior of spring-like motifs tethered to a DNA origami nanostructure
    In: Nanoscale Vol. 9 (2017) Nr. 13, pp. 4486 - 4496
    ISSN: 2040-3372; 2040-3364
  • Streich, Carmen; Akkari, Laura; Decker, Christina; Bormann, Jenny; Rehbock, Christoph; Müller-Schiffmann, Andreas; Niemeyer, Felix; Nagel-Steger, Luitgard; Willbold, Dieter; Sacca, Barbara; Kor, Carsten
    Characterizing the Effect of Multivalent Conjugates Composed of Aβ-Specific Ligands and Metal Nanoparticles on Neurotoxic Fibrillar Aggregation
    In: ACS Nano (2016) pp. 7582 - 7597
    ISSN: 1936-086X; 1936-0851
  • Pfeifer, Wolfgang; Sacca, Barbara
    From Nano to Macro through Hierarchical Self-Assembly : The DNA Paradigm
    In: ChemBioChem Vol. 17 (2016) Nr. 12, pp. 1063 - 1080
    ISSN: 1439-4227; 1439-7633
  • Poepsel, Simon; Sprengel, Andreas; Sacca, Barbara; Kaschani, Farnusch; Kaiser, Markus; Gatsogiannis, Christos; Raunser, Stefan; Clausen, Tim; Ehrmann, Michael
    Determinants of amyloid fibril degradation by the PDZ protease HTRA1
    In: Nature Chemical Biology Vol. 11 (2015) Nr. 11, pp. 862 - 869
    ISSN: 1552-4450; 1552-4469
  • Sacca, Barbara; Ishitsuka, Yuji; Meyer, Rebecca; Sprengel, Andreas; Schöneweiß, Elisa-Charlott; Nienhaus, G. Ulrich; Niemeyer, Christof M.
    Reversible reconfiguration of DNA origami nanochambers monitored by single-molecule FRET
    In: Angewandte Chemie International Edition Vol. 54 (2015) Nr. 12, pp. 3592 - 3597
    ISSN: 1433-7851; 1521-3773
  • Meyer, Rebecca; Sacca, Barbara; Niemeyer, Christof M.
    Site-Directed, on-surface assembly of DNA nanostructures
    In: Angewandte Chemie International Edition Vol. 54 (2015) Nr. 41, pp. 12039 - 12043
    ISSN: 1433-7851
  • Erkelenz, Michael; Bauer, Dennis M.; Meyer, Rebecca; Gatsogiannis, Christos; Raunser, Stefan; Sacca, Barbara; Niemeyer, Christof M.
    A facile method for preparation of tailored scaffolds for DNA-origami
    In: Small Vol. 10 (2014) Nr. 1, pp. 73 - 77
    ISSN: 1613-6810; 1613-6829
  • Sacca, Barbara
    Nucleic Acids Nanotechnology
    In: Methods: A Companion to Methods in Enzymology Vol. 67 (2014) Nr. 2, pp. 103 - 104
    ISSN: 1046-2023; 1095-9130
  • Sacca, Barbara; Niemeyer, Christof M.
    DNA Origami : The Art of Folding DNA
    In: Angewandte Chemie International Edition Vol. 51 (2012) Nr. 1, pp. 58 - 66
    ISSN: 1521-3773; 0570-0833
  • Tennstaedt, Annette; Pöpsel, Simon; Trübestein, Linda; Hauske, Patrick; Brockmann, Anke; Schmidt, Nina; Irle, Inga; Sacca, Barbara; Niemeyer, Christof M.; Brandt, Roland; Ksiezak-Reding, Hanna; Tirniceriu, Anca Laura; Egensperger, Rupert; Baldi, Alfonso; Dehmelt, Leif; Kaiser, Markus; Huber, Robert; Clausen, Tim; Ehrmann, Michael
    Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates
    In: Journal of Biological Chemistry Vol. 287 (2012) Nr. 25, pp. 20931 - 20941
    ISSN: 0021-9258; 1083-351X
  • Sacca, Barbara; Siebers, Benjamin; Meyer, Rebecca; Bayer, Manfred; Niemeyer, Christof M.
    Nanolattices of Switchable DNA-Based Motors
    In: Small Vol. 8 (2012) Nr. 19, pp. 3000 - 3008
    ISSN: 1613-6810; 1613-6829
  • Sacca, Barbara; Niemeyer, Christof M.
    Covalent Tethering of Protruding Arms for Addressable DNA Nanostructures
    In: Small Vol. 7 (2011) Nr. 20, pp. 2887 - 2898
    ISSN: 1613-6810; 1613-6829
  • Sacca, Barbara; Niemeyer, Christof M.
    Functionalization of DNA nanostructures with proteins
    In: Chemical Society Reviews Vol. 40 (2011) Nr. 12, pp. 5910 - 5921
    ISSN: 1460-4744; 0306-0012
  • Sacca, Barbara; Niemeyer, Christof M.; Rabe, Kersten; Schroeder, Hendrik; Arndt, Andreas; Kiko, Kathrin; Erkelenz, Michael; Meyer, Rebecca
    Orthogonal Protein Decoration of DNA Origami
    In: Angewandte Chemie International Edition Vol. 49 (2010) Nr. 49, pp. 9378 - 9383
    ISSN: 1521-3773
  • Sacca, Barbara; Meyer, Rebecca; Niemeyer, Christof M.
    Analysis of the Self-Assembly of 4×4 DNA Tiles by Temperature-Dependent FRET Spectroscopy
    In: ChemPhysChem Vol. 18 (2009) Nr. 18, pp. 3239 - 3248
    ISSN: 1439-7641; 1439-4235
  • Feldkamp, Udo; Niemeyer, Christof M.; Sacca, Barbara
    Dendritic DNA Building Blocks for Amplified Detection Assays and Biomaterials
    In: Angewandte Chemie International Edition Vol. 48 (2009) Nr. 33, pp. 5996 - 6000
    ISSN: 1521-3773; 0570-0833
  • Sacca, Barbara; Niemeyer, Christof M.; Meyer, Rebecca
    Temperature-dependent FRET spectroscopy for the high-throughput analysis of self-assembled DNA nanostructures in real time
    In: Nature protocols Vol. 4 (2009) Nr. 3, pp. 271 - 285
    ISSN: 1750-2799
  • De Cian, Anne; Gros, Julien; Guédin, Aurore; Haddi, Meriem; Lyonnais, Sébastien; Guittat, Lionel; Riou, Jean-François; Trentesaux, Chantal; Sacca, Barbara; Lacroix, Laurent; Alberti, Patrizia; Mergny, Jean-Louis
    DNA and RNA quadruplex ligands
    In: Nucleic Acids Symposium Series Vol. 52 (2008) Nr. 1, pp. 7 - 8
    ISSN: 1746-8272
  • Sacca, Barbara; Niemeyer, Christof M.; Schroeder, Hendrik; Feldkamp, Udo; Meyer, Rebecca
    High-Throughput, Real-Time Monitoring of the Self-Assembly of DNA Nanostructures by FRET Spectroscopy
    In: Angewandte Chemie International Edition Vol. 47 (2008) Nr. 11, pp. 2135 - 2137
    ISSN: 1521-3773; 0570-0833
  • Rao, V. Ashutosh; Conti, Chiara; Guirouilh-Barbat, Josee; Nakamura, Asako; Miao, Ze-Hong; Davies, Sally L.; Sacca, Barbara; Hickson, Ian D.; Bensimon, Aaron; Pommier, Yves
    Endogenous γ-H2AX-ATM-Chk2 checkpoint activation in Bloom's syndrome helicase-deficient cells is related to DNA replication arrested forks
    In: Molecular Cancer Research Vol. 5 (2007) Nr. 7, pp. 713 - 724
    ISSN: 1541-7786
  • De Cian, Anne; Guittat, Lionel; Kaiser, Markus; Sacca, Barbara; Amrane, Samir; Bourdoncle, Anne; Alberti, Patrizia; Teulade-Fichou, Marie-Paule; Lacroix, Laurent; Mergny, Jean-Louis
    Fluorescence-based melting assays for studying quadruplex ligands
    In: Methods: A Companion to Methods in Enzymology Vol. 42 (2007) Nr. 2, pp. 183 - 195
    ISSN: 1046-2023; 1095-9130
  • Conti, Chiara; Sacca, Barbara; Herrick, John; Lalou, Claude; Pommier, Yves; Bensimon, Aaron
    Replication fork velocities at adjacent replication origins are coordinately modified during DNA replication in human cells
    In: Molecular Biology of the Cell Vol. 18 (2007) Nr. 8, pp. 3059 - 3067
    ISSN: 1939-4586
  • Alberti, Patrizia; Bourdoncle, Anne; Sacca, Barbara; Lacroix, Laurent; Mergny, Jean-Louis
    DNA nanomachines and nanostructures involving quadruplexes
    In: Organic and Biomolecular Chemistry Vol. 4 (2006) Nr. 18, pp. 3383 - 3391
    ISSN: 1477-0539; 1477-0520
  • Mergny, Jean-Louis; De Cian, Anne; Ghelab, Amar; Sacca, Barbara; Lacroix, Laurent
    Kinetics of tetramolecular quadruplexes
    In: Nucleic Acids Research Vol. 33 (2005) Nr. 1, pp. 81 - 94
    ISSN: 1362-4962
  • Amrane, Samir; Sacca, Barbara; Mills, Martin; Chauhan, Madhu; Klump, Horst H.; Mergny, Jean-Louis
    Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats
    In: Nucleic Acids Research Vol. 33 (2005) Nr. 13, pp. 4065 - 4077
    ISSN: 1362-4962; 1362-4962
  • Sacca, Barbara; Lacroix, Laurent; Mergny, Jean-Louis
    The effect of chemical modifications on the thermal stability of different G-quadruplex-forming oligonucleotides
    In: Nucleic Acids Research Vol. 33 (2005) Nr. 4, pp. 1182 - 1192
    ISSN: 1362-4962; 1362-4962
  • Sinner, Eva-Kathrin; Reuning, Ute; Kök, Fatma Nese; Sacca, Barbara; Moroder, Luis; Knoll, Wolfgang; Oesterhelt, Dieter
    Incorporation of integrins into artificial planar lipid membranes: Characterization by plasmon-enhanced fluorescence spectroscopy
    In: Analytical Biochemistry Vol. 333 (2004) Nr. 2, pp. 216 - 224
    ISSN: 1096-0309
  • Renner, Christian; Sacca, Barbara; Moroder, Luis
    Synthetic Heterotrimeric Collagen Peptides as Mimics of Cell Adhesion Sites of the Basement Membrane
    In: Biopolymers - Peptide Science Section Vol. 76 (2004) Nr. 1, pp. 34 - 47
    ISSN: 1097-0282
  • Sacca, Barbara; Fiori, Stella; Moroder, Luis
    Studies of the local conformational properties of the cell-adhesion domain of collagen type IV in synthetic heterotrimeric peptides
    In: Biochemistry (US) Vol. 42 (2003) Nr. 12, pp. 3429 - 3436
    ISSN: 1520-4995
  • Sacca, Barbara; Sinner, Eva-Kathrin; Kaiser, Jens; Lübken, Christoph; Eble, Johannes A.; Moroder, Luis
    Binding and docking of synthetic heterotrimeric collagen type IV peptides with α1β1 integrin
    In: ChemBioChem Vol. 3 (2002) Nr. 9, pp. 904 - 907
    ISSN: 1439-4227; 1439-7633
  • Sacca, Barbara; Barth, Dirk; Musiol, Hans-Jürgen; Moroder, Luis
    Conformation-dependent side reactions in interstrand-disulfide bridging of trimeric collagenous peptides by regioselective cysteine chemistry
    In: Journal of Peptide Science Vol. 8 (2002) Nr. 5, pp. 205 - 210
    ISSN: 1099-1387
  • Fiori, Stella; Sacca, Barbara; Moroder, Luis
    Structural properties of a collagenous heterotrimer that mimics the collagenase cleavage site of collagen type I
    In: Journal of Molecular Biology Vol. 319 (2002) Nr. 5, pp. 1235 - 1242
    ISSN: 0022-2836
  • Sacca, Barbara; Moroder, Luis
    Synthesis of heterotrimeric collagen peptides containing the α 1 β 1 integrin recognition site of collagen type IV
    In: Journal of Peptide Science Vol. 8 (2002) Nr. 5, pp. 192 - 204
    ISSN: 1099-1387
  • Sacca, Barbara; Renner, Christian; Moroder, Luis
    The chain register in heterotrimeric collagen peptides affects triple helix stability and folding kinetics
    In: Journal of Molecular Biology Vol. 324 (2002) Nr. 2, pp. 309 - 318
    ISSN: 0022-2836

ZMB Research Programme

Prof. Dr. Barbara Saccà participates in the ZMB research programme III, Molecular and Chemical Cell Biology.