ZMB Member Bernd Giebel

ZMB Member
Bernd Giebel

Next ZMB-Member
Bernd Giebel portrait
© UDE/Bettina Engel-Albustin

Prof. Dr. Bernd Giebel

Giebel Lab

Institute for Transfusion Medicine
University Hospital Essen
Hufelandstraße 55
45122 Essen

Research Overview

The group of Bernd Giebel investigates lineage relationships of early human hematopoietic progenitors as well as mechanisms controlling their self-renewal and lineage specification. In this context the group is especially interested in dissecting roles of asymmetric cell division, which they have proven to occur in at least a proportion of the cells. Since 2009, as a second topic the group is also working in the fast expanding field of extracellular vesicles (EVs), often termed exosomes or microvesicles, respectively. Here, the group is especially interested in deciphering the therapeutic potential of EVs released from mesenchymal stem/stromal cells (MSCs). After demonstrating therapeutic potentials in different systems including the treatment of a graft-versus-host disease patient, the group now aims to optimize and qualify the MSC-EV production for the clinical setting.
Keywords: Hematopoietic stem cells, hematopoiesis, mesenchymal stem cells, extracellular vesicles, exosomes
Read more

Selected Publications

    Journal articles

  • Rolandsson Enes, Sara; Dzneladze, Irakli; Hampton, Thomas H.; Neff, Samuel L.; Asarian, Lori; Barua, Jayita; Tertel, Tobias; Giebel, Bernd; Pereyra, Nicolas; McKenna, David H.; Hu, Pingzhao; Acton, Erica; Ashare, Alix; Liu, Kathleen D.; Krasnodembskaya, Anna D.; English, Karen; Stanton, Bruce A.; Rocco, Patricia R.M.; Matthay, Michael A.; dos Santos, Claudia C.; Weiss, Daniel J.
    Acute respiratory distress vs healthy lung environments differently affect mesenchymal stromal cell extracellular vesicle miRNAs
    In: Cytotherapy , Vol. 27 2025 , Nr. 5 , pp. 581 - 596
    DOI (Open Access)
  • Giebel, Bernd; Lim, Sai Kiang
    Overcoming challenges in MSC-sEV therapeutics : Insights and advances after a decade of research
    In: Cytotherapy 2025 , in press
    DOI (Open Access)
  • Mouloud, Yanis; Staubach, Simon; Stambouli, Oumaima; Mokhtari, Shakiba; Kutzner, Tanja J; Zwanziger, Denise; Hemeda, Hatim; Giebel, Bernd
    Calcium chloride declotted human platelet lysate promotes the expansion of mesenchymal stromal cells and allows manufacturing of immunomodulatory active extracellular vesicle products
    In: Cytotherapy , Vol. 26 2024 , Nr. 9 , pp. 988 - 998
    DOI (Open Access)
  • Barekzai, Jan; Refflinghaus, Laura; Okpara, Maduwuike; Tasto, Lars; Tertel, Tobias; Giebel, Bernd; Czermak, Peter; Salzig, Denise
    Process development for the production of mesenchymal stromal cell-derived extracellular vesicles in conventional 2D systems
    In: Cytotherapy , Vol. 26 2024 , Nr. 9 , pp. 999 - 1012
    DOI (Open Access)
  • Toh, Wei Seong; Yarani, Reza; El Andaloussi, Samir; Cho, Byong Seung; Choi, Chulhee; Corteling, Randolph; De Fougerolles, Antonin; Gimona, Mario; Herz, Josephine; Khoury, Maroun; Robbins, Paul D.; Williams, Douglas; Weiss, Daniel J.; Rohde, Eva; Giebel, Bernd; Lim, Sai Kiang
    A report on the International Society for Cell & Gene Therapy 2022 Scientific Signature Series, “Therapeutic advances with native and engineered human extracellular vesicles”
    In: Cytotherapy , Vol. 25 2023 , Nr. 8 , pp. 810 - 814
    DOI
  • Bauer, Fabiola Nardi; Tertel, Tobias; Stambouli, Oumaima; Wang, Chen; Dittrich, Robin; Staubach, Simon; Börger, Verena; Hermann, Dirk M.; Brandau, Sven; Giebel, Bernd
    CD73 activity of mesenchymal stromal cell-derived extracellular vesicle preparations is detergent-resistant and does not correlate with immunomodulatory capabilities
    In: Cytotherapy , Vol. 25 2023 , Nr. 2 , pp. 138 - 147
    DOI (Open Access)
  • Madel, Rabea J.; Börger, Verena; Dittrich, Robin; Bremer, Michel; Tertel, Tobias; Phuong, Nhi Ngo Thi; Baba, Hideo Andreas; Kordelas, Lambros; Staubach, Simon; Stein, Frank; Haberkant, Per; Hackl, Matthias; Grillari, Regina; Grillari, Johannes; Buer, Jan; Horn, Peter; Westendorf, Astrid; Brandau, Sven; Kirschning, Carsten Jürgen; Giebel, Bernd
    Independent human mesenchymal stromal cell–derived extracellular vesicle preparations differentially attenuate symptoms in an advanced murine graft-versus-host disease model
    In: Cytotherapy 2023
    DOI (Open Access)
  • Bremer, Michel; Nardi Bauer, Fabiola; Tertel, Tobias; Dittrich, Robin; Horn, Peter; Börger, Verena; Giebel, Bernd
    Qualification of a multidonor mixed lymphocyte reaction assay for the functional characterization of immunomodulatory extracellular vesicles
    In: Cytotherapy , Vol. 25 2023 , Nr. 8 , pp. 847 - 857
    DOI
  • Tertel, Tobias; Schoppet, Melanie; Stambouli, Oumaima; Al-Jipouri, Ali; James, Patrick F.; Giebel, Bernd
    Imaging flow cytometry challenges the usefulness of classically used extracellular vesicle labeling dyes and qualifies the novel dye Exoria for the labeling of mesenchymal stromal cell–extracellular vesicle preparations
    In: Cytotherapy , Vol. 24 2022 , Nr. 6 , pp. 619 - 628
    DOI (Open Access)
  • Wang, Chen; Börger, Verena; Mohamud Yusuf, Ayan; Tertel, Tobias; Stambouli, Oumaima; Murke, Florian; Freund, Nico; Kleinschnitz, Christoph; Herz, Josephine; Gunzer, Matthias; Popa-Wagner, Aurel; Döppner, Thorsten R.; Giebel, Bernd; Hermann, Dirk M.
    Postischemic Neuroprotection Associated With Anti-Inflammatory Effects by Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles in Aged Mice
    In: Stroke , Vol. 53 2022 , Nr. 1 , pp. E14 - E18
    DOI (Open Access)
  • Hermann, Dirk M.; Döppner, Thorsten R.; Giebel, Bernd
    Circulating MicroRNAs : Posttranscriptional Regulators and Disease Markers Holding Promise in Stroke Prediction
    In: Stroke , Vol. 52 2021 , Nr. 3 , pp. 954 - 956
    DOI (Open Access)
  • Gimona, Mario; Brizzi, Maria Felice; Choo, Andre Boon Hwa; Dominici, Massimo; Davidson, Sean M.; Grillari, Johannes; Hermann, Dirk M.; Hill, Andrew F.; de Kleijn, Dominique; Lai, Ruenn Chai; Lai, Charles P.; Lim, Rebecca; Monguió-Tortajada, Marta; Muraca, Maurizio; Ochiya, Takahiro; Ortiz, Luis A.; Toh, Wei Seong; Yi, Yong Weon; Witwer, Kenneth W.; Giebel, Bernd; Lim, Sai Kiang
    Critical considerations for the development of potency tests for therapeutic applications of mesenchymal stromal cell-derived small extracellular vesicles
    In: Cytotherapy , Vol. 23 2021 , Nr. 5 , pp. 373 - 380
    DOI (Open Access)
  • Stambouli, O.; Dittrich, Robin; Nardi-Bauer, Fabiola; Tertel, Tobias; Horn, Peter; Giebel, Bernd
    Impact of mesenchymal stromal/stem cell isolation strategies on the immunomodulatory activity of their extracellular vesicles
    In: Cytotherapy , Vol. 23 2021 , Nr. Suppl. 5 , pp. 113
  • Hermann, Dirk M.; Döppner, Thorsten R.; Giebel, Bernd
    New Light on the Horizon : Extracellular Vesicles as Diagnostic Tool in Transient Ischemic Attack and Ischemic Stroke
    In: Stroke , Vol. 52 2021 , Nr. 10 , pp. 3348 - 3350
    DOI (Open Access)
  • Börger, Verena; Weiss, Daniel J.; Anderson, Johnathon D.; Borràs, Francesc E.; Bussolati, Benedetta; Carter, David R.F.; Dominici, Massimo; Falcón-Pérez, Juan M.; Gimona, Mario; Hill, Andrew F.; Hoffman, Andrew M.; de Kleijn, Dominique; Levine, Bruce L.; Lim, Rebecca; Lötvall, Jan; Mitsialis, S. Alex; Monguió-Tortajada, Marta; Muraca, Maurizio; Nieuwland, Rienk; Nowocin, Anna; O'Driscoll, Lorraine; Ortiz, Luis A.; Phinney, Donald G; Reischl, Ilona; Rohde, Eva; Sanzenbacher, Ralf; Théry, Clotilde; Toh, Wei Seong; Witwer, Kenneth W.; Lim, Sai Kiang; Giebel, Bernd
    International Society for Extracellular Vesicles and International Society for Cell and Gene Therapy statement on extracellular vesicles from mesenchymal stromal cells and other cells : considerations for potential therapeutic agents to suppress coronavirus disease-19
    In: Cytotherapy , Vol. 22 2020 , Nr. 9 , pp. 482 - 485
    DOI (Open Access)
  • Wang, Chen; Börger, Verena; Sardari, Maryam; Murke, Florian; Skuljec, Jelena; Pul, Refik; Hagemann, Nina; Dzyubenko, Egor; Dittrich, Robin; Gregorius, Jonas; Hasenberg, Mike; Kleinschnitz, Christoph; Popa-Wagner, Aurel; Döppner, Thorsten R.; Gunzer, Matthias; Giebel, Bernd; Hermann, Dirk M.
    Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Induce Ischemic Neuroprotection by Modulating Leukocytes and Specifically Neutrophils
    In: Stroke , Vol. 9 2020 , Nr. 10 , pp. 1825 - 1834
    DOI (Open Access)
  • Sokolova, Viktoriya; Nzou, Goodwell; van der Meer, Selina; Ruks, Tatjana; Heggen, Marc; Loza, Kateryna; Hagemann, Nina; Murke, Florian; Giebel, Bernd; Hermann, Dirk M.; Atala, Anthony J.; Epple, Matthias
    Ultrasmall gold nanoparticles (2 nm) can penetrate and enter cell nuclei in an in vitro 3D brain spheroid model
    In: Acta Biomaterialia , Vol. 111 2020 , pp. 349 - 362
    DOI
  • Bremer, M.; Börger, Verena; Horn, Peter; Giebel, Bernd
    A mixed lymphocyte reaction as a functional assay for extracellular vesicles of different origins
    In: Cytotherapy , Vol. 21 2019 , Nr. 5 , pp. 57
    DOI
  • Staubach, S.; Reiter, C.; Weber, G.; Giebel, Bernd
    Free flow electrophoresis allows preparation of extracellular vesicles with high purity
    In: Cytotherapy , Vol. 21 2019 , Nr. Suppl. 5 , pp. 57 - S58
    DOI
  • Madel, R.; Borger, V.; Bremer, M.; Horn, Peter; Baba, Hideo Andreas; Brandau, Sven; Buer, Jan; Giebel, Bernd; Kirschning, Carsten Jürgen
    MSC-EVS protect mice from graft-versus-host disease pathology in a preparation dependent manner
    In: Cytotherapy , Vol. 21 2019 , Nr. Suppl. 5 , pp. 57
    DOI
  • Börger, Verena; Dittrich, Ralf; Staubach, S.; Zumegen, S.; Horn, Peter; Giebel, Bernd
    Tangential flow filtration, a potential method for the scaled preparation of extracellular vesicles
    In: Cytotherapy , Vol. 21 2019 , Nr. Suppl. 5 , pp. 57
    DOI
  • Van Deun, Jan; Mestdagh, Pieter; Agostinis, Patrizia; Akay, Özden; Anand, Sushma; Anckaert, Jasper; Martinez, Zoraida Andreu; Baetens, Tine; Beghein, Els; Bertier, Laurence; Berx, Geert; Boere, Janneke; Boukouris, Stephanie; Bremer, Michel; Buschmann, Dominik; Byrd, James B.; Casert, Clara; Cheng, Lesley; Cmoch, Anna; Daveloose, Delphine; De Smedt, Eva; Demirsoy, Seyma; Depoorter, Victoria; Dhondt, Bert; Driedonks, Tom A.P.; Dudek, Aleksandra; Elsharawy, Abdou; Floris, Ilaria; Foers, Andrew D.; Gärtner, Kathrin; Garg, Abhishek D.; Geeurickx, Edward; Gettemans, Jan; Ghazavi, Farzaneh; Giebel, Bernd; Kormelink, Tom Groot; Hancock, Grace; Helsmoortel, Hetty; Hill, Andrew F.; Hyenne, Vincent; Kalra, Hina; Kim, David; Kowal, Joanna; Kraemer, Sandra; Leidinger, Petra; Leonelli, Carina; Liang, Yaxuan; Lippens, Lien; Liu, Shu; Lo Cicero, Alessandra; Martin, Shaun; Mathivanan, Suresh; Mathiyalagan, Prabhu; Matusek, Támas; Milani, Gloria; Monguió-Tortajada, Marta; Mus, Liselot M.; Muth, Dillon C.; Németh, Andrea; Nolte-'T Hoen, Esther N.M.; O'Driscoll, Lorraine; Palmulli, Roberta; Pfaffl, Michael W.; Primdal-Bengtson, Bjarke; Romano, Erminia; Rousseau, Quentin; Sahoo, Susmita; Sampaio, Natalia; Samuel, Monisha; Scicluna, Benjamin; Soen, Bieke; Steels, Anneleen; Swinnen, Johannes V.; Takatalo, Maarit; Thaminy, Safia; Théry, Clotilde; Tulkens, Joeri; Van Audenhove, Isabel; Van Der Grein, Susanne; Van Goethem, Alan; Van Herwijnen, Martijn J.; Van Niel, Guillaume; Van Roy, Nadine; Van Vliet, Alexander R.; Vandamme, Niels; Vanhauwaert, Suzanne; Vergauwen, Glenn; Verweij, Frederik; Wallaert, Annelynn; Wauben, Marca; Witwer, Kenneth W.; Zonneveld, Marijke I.; De Wever, Olivier; Vandesompele, Jo; Hendrix, An
    EV-TRACK : Transparent reporting and centralizing knowledge in extracellular vesicle research
    In: Nature Methods , Vol. 14 2017 , Nr. 3 , pp. 228 - 232
    DOI
  • König, Lisa; Rebmann, Vera; Hoffmann, Oliver; Bittner, A. K.; Wagner, B.; Manvailer, L. F. S.; Schramm, S.; Bankfalvi, Agnes; Giebel, Bernd; Kimmig, Rainer; Horn, Peter A.; Kasimir-Bauer, Sabine
    Breast cancer-derived extracellular vesicles : clinical and prognostic impact
    In: Cancer Research , Vol. 76 2016 , Nr. 14 Suppl. , pp. 3152
    DOI
  • Fais, Stefano; O'Driscoll, Lorraine; Borras, Francesc E.; Buzas, Edit; Camussi, Giovanni; Cappello, Francesco; Carvalho, Joana; Cordeiro Da Silva, Anabela; Del Portillo, Hernando; El Andaloussi, Samir; Ficko Trček, Tanja; Furlan, Roberto; Hendrix, An; Gursel, Ihsan; Kralj-Iglic, Veronika; Kaeffer, Bertrand; Kosanovic, Maja; Lekka, Marilena E.; Lipps, Georg; Logozzi, Mariantonia; Marcilla, Antonio; Sammar, Marei; Llorente, Alicia; Nazarenko, Irina; Oliveira, Carla; Pocsfalvi, Gabriella; Rajendran, Lawrence; Raposo, Graça; Rohde, Eva; Siljander, Pia; Van Niel, Guillaume; Vasconcelos, M. Helena; Yáñez-Mó, María; Yliperttula, Marjo L.; Zarovni, Natasa; Zavec, Apolonija Bedina; Giebel, Bernd
    Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
    In: ACS Nano , Vol. 10 2016 , Nr. 4 , pp. 3886 - 3899
    DOI
  • Hönes, J M; Botezatu, L; Helness, A; Vadnais, C; Vassen, L; Robert, F; Hergenhan, S M; Thivakaran, A; Schütte, J; Al-Matary, Y S; Lams, R F; Fraszscak, J; Makishima, H; Radivoyevitch, T; Przychodzen, B; da Conceição Castro, S V; Görgens, A; Giebel, Bernd; Klein-Hitpaß, Ludger; Lennartz, K; Heuser, M; Thiede, C; Ehninger, G; Dührsen, Ulrich; Maciejewski, J; Möröy, T; Khandanpour, Cyrus
    GFI1 as a novel prognostic and therapeutic factor for AML/MDS
    In: Leukemia , Vol. 30 2016 , Nr. 6 , pp. 1237 - 1245
    DOI
  • Radtke, Stefan; Görgens, André; Kordelas, Lambros; Schmidt, Markus; Kimmig, Rainer; Köninger, Angela; Horn, Peter; Giebel, Bernd
    CD133 allows elaborated discrimination and quantification of haematopoietic progenitor subsets in human haematopoietic stem cell transplants
    In: British Journal of Haematology , Vol. 169 2015 , Nr. 6 , pp. 868 - 878
    DOI
  • Goergens, Andre; Ludwig, Anna-Krist; Moellmann, Michael; Krawczyk, Adalbert; Dürig, Jan; Hanenberg, Helmut; Horn, Peter A.; Giebel, Bernd
    Multipotent Hematopoietic Progenitors Divide Asymmetrically to Create Progenitors of the Lymphomyeloid and Erythromyeloid Lineages
    In: Stem Cell Reports , Vol. 5 2015 , Nr. 1 , pp. 154 - 155
    DOI (Open Access)
  • Malyar, Mohammad; Radtke, Stefan; Malyar, Khalil; Arjumand, Jawed; Horn, Peter; Kröger, Knut; Freisinger, Eva; Reinecke, Holger; Giebel, Bernd; Brock, Franz-Eduard
    Autologous bone marrow mononuclear cell therapy improves symptoms in patients with end-stage peripheral arterial disease and reduces inflammation-associated parameters
    In: Cytotherapy , Vol. 16 2014 , Nr. 9 , pp. 1270 - 1279
    DOI
  • Hemeda, Hatim; Giebel, Bernd; Wagner, Wolfgang
    Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells
    In: Cytotherapy , Vol. 16 2014 , Nr. 2 , pp. 170 - 180
    DOI
  • Kordelas, L; Rebmann, Vera; Ludwig, A-K; Radtke, Stefan; Ruesing, J; Döppner, Thorsten R.; Epple, Matthias; Horn, Peter; Beelen, Dietrich; Giebel, Bernd
    MSC-derived exosomes : a novel tool to treat therapy-refractory graft-versus-host disease
    In: Leukemia , Vol. 28 2014 , Nr. 4 , pp. 970 - 973
    DOI
  • Gorgens, A.; Ludwig, A. K.; Mollmann, M.; Krawczyk, Adalbert; Dürig, Jan; Hanenberg, H.; Horn, Peter; Giebel, Bernd
    Multipotent hematopoietic progenitors divide asymmetrically to create progenitors of the lymphomyeloid and erythromyeloid lineages
    In: Stem Cell Reports , Vol. 3 2014 , Nr. 6 , pp. 1058 - 1072
    DOI (Open Access)
  • Görgens, André; Radtke, Stefan; Möllmann, Michael; Cross, Michael; Dürig, Jan; Horn, Peter; Giebel, Bernd
    Revision of the Human Hematopoietic Tree: Granulocyte Subtypes Derive from Distinct Hematopoietic Lineages
    In: Cell Reports , Vol. 3 2013 , Nr. 5 , pp. 1539 - 1552
    DOI
  • Weide, Benjamin; Schilling, Bastian; Paschen, Annette; Görgens, Andre; Sucker, Antje; Zhao, Fang; Giebel, Bernd; Griewank, Klaus; Schadendorf, Dirk
    Vemurafenib reverses immunosuppression by myeloid derived suppressor cells
    In: Cancer Research , Vol. 73 2013 , Nr. 8 Suppl. , pp. 4714
    DOI
  • Goebel, Maria; Möllmann, Michael; Gorgens, A.; Dührsen, Ulrich; Huttmann, A.; Giebel, Bernd; Unger, A.; Choidas, A.; Dürig, Jan
    Preclinical Testing of a Novel Axl-Kinase Inhibitor in Chronic Lymphocytic Leukemia
    In: Blood , Vol. 120 2012 , Nr. 21
Read more