ZMB Member George Iliakis

ZMB Member
George Iliakis

Next ZMB-Member
George Iliakis portrait

Prof. Dr. George Iliakis

University Hospital Essen
Hufelandstr. 55
45122 Essen

Research Overview

Cellular responses to DNA damage
The genomes of eukaryotic cells are under continuous assault by cosmic and terrestrial radiation sources, as well as the byproducts of the intracellular metabolism such as reactive oxygen species and replication errors that lead to the generation of DNA double strand breaks (DSBs). In addition to accidental events, DSBs are also generated during scheduled biological processes such as meiosis or V(D)J recombination and during diagnostic procedures utilizing ionizing radiation. Finally, therapeutic interventions in the therapy of cancer, such as exposure to ionizing radiation and treatment with certain chemotherapeutic agents, leads to the generation of DSBs. DSBs are severe lesions with a high probability of conversion to lethal or mutagenic events. Therefore, eukaryotic cells have developed efficient repair pathways to remove DSBs from their genome. These repair activities are supported by signaling pathways, termed checkpoints that register the presence of DSBs and arrest or delay cell cycle progression in G1, S, or G2 phase to prevent modifications compromising repair or generating lethal events.

  • Evaluation of the relative contribution of the different pathways implicated in double strand break repair
  • Elucidation of the contribution of checkpoints in double strand break repair
  • Molecular mechanisms of hyperthermia induced checkpoint activation
  • Radiation effects in mouse embryonic tissue with emphasis on genomic instability

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Selected Publications

    Journal articles

  • Soni, Aashish; Lin, Xixi; Mladenov, Emil; Mladenova, Veronika; Stuschke, Martin; Iliakis, George
    BMN673 Is a PARP Inhibitor with Unique Radiosensitizing Properties: : Mechanisms and Potential in Radiation Therapy
    In: Cancers Vol. 14 (2022) Nr. 22, 5619
  • Tremi, Ioanna; Havaki, Sophia; Georgitsopoulou, Sofia; Terzoudi, Georgia; Lykakis, Ioannis N.; Iliakis, George; Georgakilas, Vasilios; Gorgoulis, Vassilis G.; Georgakilas, Alexandros G.
    Biological Response of Human Cancer Cells to Ionizing Radiation in Combination with Gold Nanoparticles
    In: Cancers Vol. 14 (2022) Nr. 20, 5086
  • Hassiepen, Christina; Soni, Aashish; Rudolf, Ines; Boron, Vivian; Oeck, Sebastian; Iliakis, George; Schramm, Alexander
    NTRK1/TrkA activation overrides the G₂ /M-checkpoint upon irradiation
    In: Cancers Vol. 13 (2021) Nr. 23, 6023
  • Iliakis, George E.
    New players in the regulation of DNA-PK activity : Survivin joins the crowd
    In: Cancer Research Vol. 81 (2021) Nr. 9, pp. 2270 - 2271
  • Wang, Huichen; Perrault, Ange Ronel; Takeda, Yoshihiko; Qin, Wei; Wang, Hongyan; Iliakis, George
    Biochemical evidence for Ku-independent backup pathways of NHEJ
    In: Nucleic Acids Research Vol. 48 (2020) Nr. 9, pp. 5200
  • Wang, Xiang; Guan, Jun; Hu, Baocheng; Weiss, Robert S.; Iliakis, George; Wang, Ya
    Involvement of Hus1 in the chain elongation step of DNA replication after exposure to camptothecin or ionizing radiation
    In: Nucleic Acids Research Vol. 48 (2020) Nr. 6, pp. 3396
  • Mladenov, Emil; Staudt, Christian; Soni, Aashish; Murmann-Konda, Tamara; Siemann-Loekes, Maria; Iliakis, George
    Strong suppression of gene conversion with increasing DNA double-strand break load delimited by 53BP1 and RAD52
    In: Nucleic Acids Research Vol. 48 (2020) Nr. 4, pp. 1905 - 1924
  • Iliakis, George; Mladenov, Emil; Mladenova, Veronika
    Necessities in the processing of DNA double strand breaks and their effects on genomic instability and cancer
    In: Cancers Vol. 11 (2019) Nr. 11, pp. 1671
  • Schipler, Agnes; Mladenova, Veronika; Soni, Aashish; Nikolov, Vladimir; Saha, Janapriya; Mladenov, Emil; Iliakis, George
    Chromosome thripsis by DNA double strand break clusters causes enhanced cell lethality, chromosomal translocations and 53BP1-recruitment
    In: Nucleic Acids Research Vol. 44 (2016) Nr. 16, pp. 7673 - 7690
  • van den Boom, Johannes; Wolf, Markus; Weimann, Lena; Schulze, Nina; Li, Fanghua; Kaschani, Farnusch; Riemer, Anne; Zierhut, Christian; Kaiser, Markus; Iliakis, George; Funabiki, Hironori; Meyer, Hemmo
    VCP/p97 Extracts Sterically Trapped Ku70/80 Rings from DNA in Double-Strand Break Repair
    In: Molecular Cell Vol. 64 (2016) Nr. 1, pp. 189 - 198
  • Iliakis, George; Magin, Simon
    Balance shift between error-free and error prone DNA double-strand break repair as a novel mechanism of radiosensitization by nucleoside analogs
    In: Cancer Research Vol. 75 (2015) Nr. Suppl. 15, pp. 3629
  • Costantino, L.; Sotiriou, S. K.; Rantala, J. K.; Magin, S.; Mladenov, E.; Helleday, T.; Haber, J. E.; Iliakis, George; Kallioniemi, O. P.; Halazonetis, T. D.
    Break-induced replication repair of damaged forks induces genomic duplications in human cells
    In: Science Vol. 343 (2014) Nr. 6166, pp. 88 - 91
  • Soni, Aashish; Siemann, Maria; Grabos, Martha; Murmann, Tamara; Pantelias, Gabriel E; Iliakis, George
    Requirement for Parp-1 and DNA ligases 1 or 3 but not of Xrcc1 in chromosomal translocation formation by backup end joining
    In: Nucleic Acids Research Vol. 42 (2014) Nr. 10, pp. 6380 - 6392
  • Schipler, Agnes; Iliakis, George
    DNA double-strand-break complexity levels and their possible contributions to the probability for error-prone processing and repair pathway choice
    In: Nucleic Acids Research Vol. 41 (2013) Nr. 16, pp. 7589 - 7605
  • Arakawa, Hiroshi; Bednar, Theresa; Wang, Minli; Paul, Katja; Mladenov, Emil; Bencsik-Theilen, Alena A.; Iliakis, George
    Functional redundancy between DNA ligases i and III in DNA replication in vertebrate cells
    In: Nucleic Acids Research Vol. 40 (2012) Nr. 6, pp. 2599 - 2610
  • Singh, Satyendra K.; Wang, Minli; Staudt, Christian; Iliakis, George
    Post-irradiation chemical processing of DNA damage generates double-strand breaks in cells already engaged in repair
    In: Nucleic Acids Research Vol. 39 (2011) Nr. 19, pp. 8416 - 8429
  • Klammer, Holger; Kadhim, Munira; Iliakis, George
    Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells
    In: Cancer Research Vol. 70 (2010) Nr. 21, pp. 8498 - 8506
  • Kinner, Andrea; Wu, Wenqi; Staudt, Christian; Iliakis, George
    Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin.
    In: Nucleic Acids Research Vol. 36 (2008) Nr. 17, pp. 5678 - 5694
  • Rosidi, Bustanur; Wang, Minli; Wu, Wenqi; Sharma, Aparna; Wang, Huichen; Iliakis, George
    Histone H1 functions as a stimulatory factor in backup pathways of NHEJ
    In: Nucleic Acids Research Vol. 36 (2008) Nr. 5, pp. 1610 - 1623
  • Wang, Minli; Wu, Weizhong; Wu, Wenqi; Rosidi, Bustanur; Zhang, Lihua; Wang, Huichen; Iliakis, George
    PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways
    In: Nucleic Acids Research Vol. 34 (2006) Nr. 21, pp. 6170 - 6182
  • Terzoudi, Georgia I.; Manola, Kaliopi N.; Pantelias, Gabriel E.; Iliakis, George
    Checkpoint abrogation in G₂ compromises repair of chromosomal breaks in ataxia telangiectasia cells
    In: Cancer Research Vol. 65 (2005) Nr. 24, pp. 11292 - 11296
  • Wang, Huichen; Rosidi, Bustanur; Perrault, Ronel; Wang, Minli; Zhang, Lihua; Windhofer, Frank; Iliakis, George
    DNA ligase III as a candidate component of backup pathways of nonhomologous end joining
    In: Cancer Research Vol. 65 (2005) Nr. 10, pp. 4020 - 4030
  • Martín, Marta; Genescà, Anna; Latre, Laura; Jaco, Isabel; Taccioli, Guillermo E.; Egozcue, Josep; Blasco, María A.; Iliakis, George; Tusell, Laura
    Postreplicative joining of DNA double-strand breaks causes genomic instability in DNA-PKcs-deficient mouse embryonic fibroblasts
    In: Cancer Research Vol. 65 (2005) Nr. 22, pp. 10223 - 10232
  • Wang, Hongyan; Wang, Huichen; Powell, Simon N.; Iliakis, George; Wang, Ya
    ATR affecting cell radiosensitivity is dependent on homologous recombination repair but independent of nonhomologous end joining
    In: Cancer Research Vol. 64 (2004) Nr. 19, pp. 7139 - 7143
  • Zhou, Xiang-Yang; Wang, Xiang; Hu, Baocheng; Guan, Jun; Iliakis, George; Wang, Ya
    An ATM-independent S-phase checkpoint response involves CHK1 pathway
    In: Cancer Research Vol. 62 (2002) Nr. 6, pp. 1598 - 1603
  • Wang, Xiang; Li, Gloria C; Iliakis, George; Wang, Ya
    Ku affects the CHK1-dependent G(2) checkpoint after ionizing radiation
    In: Cancer Research Vol. 62 (2002) Nr. 21, pp. 6031 - 6034
  • Wang, Hongyan; Wang, Xiang; Zhou, Xiang-Yang; Chen, David J; Li, Gloria C; Iliakis, George; Wang, Ya
    Ku affects the ataxia and Rad 3-related/CHK1-dependent S phase checkpoint response after camptothecin treatment.
    In: Cancer Research Vol. 62 (2002) Nr. 9, pp. 2483 - 2487
  • Wang, Huichen; Zeng, Zhao-Chong; Perrault, Ange R.; Cheng, Xinbo; Qin, Wei; Iliakis, George
    Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells
    In: Nucleic Acids Research Vol. 29 (2001) Nr. 8, pp. 1653 - 1660
  • Peters, K B; Wang, H; Brown, J M; Iliakis, George
    Inhibition of DNA replication by tirapazamine
    In: Cancer Research Vol. 61 (2001) Nr. 14, pp. 5425 - 5431
  • Wang, H; Zeng, Z C; Bui, T A; DiBiase, S J; Qin, W; Xia, F; Powell, S N; Iliakis, George
    Nonhomologous end-joining of ionizing radiation-induced DNA double-stranded breaks in human tumor cells deficient in BRCA1 or BRCA2
    In: Cancer Research Vol. 61 (2001) Nr. 1, pp. 270 - 277
  • Wang, H; Guan, J; Perrault, A R; Wang, Y; Iliakis, George
    Replication protein A2 phosphorylation after DNA damage by the coordinated action of ataxia telangiectasia-mutated and DNA-dependent protein kinase
    In: Cancer Research Vol. 61 (2001) Nr. 23, pp. 8554 - 8563
  • DiBiase; Zeng; Chen; Hyslop; Curran; Iliakis, George
    DNA-dependent protein kinase stimulates an independently active, nonhomologous, end-joining apparatus.
    In: Cancer Research Vol. 60 (2000) Nr. 5, pp. 1245 - 1253
  • Guan, Jun; DiBiase, Steven; Iliakis, George
    The catalytic subunit DNA-dependent protein kinase (DNA-PKcs) facilitates recovery from radiation-induced inhibition of DNA replication
    In: Nucleic Acids Research Vol. 28 (2000) Nr. 5, pp. 1183 - 1192