ZMB Member George Iliakis
ZMB Member
George Iliakis
Next ZMB-Member
Prof. Dr. George Iliakis
University Hospital Essen
Hufelandstr. 55
45122 Essen
- +49 201 723 4152
- Website
- Selected Publications
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- ZMB Research Program
Oncology
Research Overview
Cellular responses to DNA damage
The genomes of eukaryotic cells are under continuous assault by cosmic and terrestrial radiation sources, as well as the byproducts of the intracellular metabolism such as reactive oxygen species and replication errors that lead to the generation of DNA double strand breaks (DSBs). In addition to accidental events, DSBs are also generated during scheduled biological processes such as meiosis or V(D)J recombination and during diagnostic procedures utilizing ionizing radiation. Finally, therapeutic interventions in the therapy of cancer, such as exposure to ionizing radiation and treatment with certain chemotherapeutic agents, leads to the generation of DSBs. DSBs are severe lesions with a high probability of conversion to lethal or mutagenic events. Therefore, eukaryotic cells have developed efficient repair pathways to remove DSBs from their genome. These repair activities are supported by signaling pathways, termed checkpoints that register the presence of DSBs and arrest or delay cell cycle progression in G1, S, or G2 phase to prevent modifications compromising repair or generating lethal events.
- Evaluation of the relative contribution of the different pathways implicated in double strand break repair
- Elucidation of the contribution of checkpoints in double strand break repair
- Molecular mechanisms of hyperthermia induced checkpoint activation
- Radiation effects in mouse embryonic tissue with emphasis on genomic instability
Selected Publications
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Biochemical evidence for Ku-independent backup pathways of NHEJIn: Nucleic Acids Research Vol. 48 (2020) Nr. 9, pp. 5200
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
Involvement of Hus1 in the chain elongation step of DNA replication after exposure to camptothecin or ionizing radiationIn: Nucleic Acids Research Vol. 48 (2020) Nr. 6, pp. 3396
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
Strong suppression of gene conversion with increasing DNA double-strand break load delimited by 53BP1 and RAD52In: Nucleic Acids Research Vol. 48 (2020) Nr. 4, pp. 1905 - 1924
ISSN: 1362-4962; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
Necessities in the processing of DNA double strand breaks and their effects on genomic instability and cancerIn: Cancers Vol. 11 (2019) Nr. 11, pp. 1671
ISSN: 2072-6694Online Full Text: dx.doi.org/ Online Full Text (Open Access) -
Chromosome thripsis by DNA double strand break clusters causes enhanced cell lethality, chromosomal translocations and 53BP1-recruitmentIn: Nucleic Acids Research Vol. 44 (2016) Nr. 16, pp. 7673 - 7690
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
VCP/p97 Extracts Sterically Trapped Ku70/80 Rings from DNA in Double-Strand Break RepairIn: Molecular Cell Vol. 64 (2016) Nr. 1, pp. 189 - 198
ISSN: 1097-4164; 1097-2765Online Full Text: dx.doi.org/ (Open Access) -
Balance shift between error-free and error prone DNA double-strand break repair as a novel mechanism of radiosensitization by nucleoside analogsIn: Cancer Research Vol. 75 (2015) Nr. Suppl. 15, pp. 3629
ISSN: 0008-5472; 1538-7445Online Full Text: dx.doi.org/ -
Break-induced replication repair of damaged forks induces genomic duplications in human cellsIn: Science Vol. 343 (2014) Nr. 6166, pp. 88 - 91
ISSN: 0036-8075Online Full Text: dx.doi.org/ Online Full Text (Open Access) -
Requirement for Parp-1 and DNA ligases 1 or 3 but not of Xrcc1 in chromosomal translocation formation by backup end joiningIn: Nucleic Acids Research Vol. 42 (2014) Nr. 10, pp. 6380 - 6392
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
DNA double-strand-break complexity levels and their possible contributions to the probability for error-prone processing and repair pathway choiceIn: Nucleic Acids Research Vol. 41 (2013) Nr. 16, pp. 7589 - 7605
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
Functional redundancy between DNA ligases i and III in DNA replication in vertebrate cellsIn: Nucleic Acids Research Vol. 40 (2012) Nr. 6, pp. 2599 - 2610
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
Post-irradiation chemical processing of DNA damage generates double-strand breaks in cells already engaged in repairIn: Nucleic Acids Research Vol. 39 (2011) Nr. 19, pp. 8416 - 8429
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cellsIn: Cancer Research Vol. 70 (2010) Nr. 21, pp. 8498 - 8506
ISSN: 0008-5472; 1538-7445Online Full Text: dx.doi.org/