ZMB Member George Iliakis

ZMB Member
George Iliakis

Next ZMB-Member
George Iliakis portrait

Prof. Dr. George Iliakis

University Hospital Essen
Hufelandstr. 55
45122 Essen

Research Overview

Cellular responses to DNA damage
The genomes of eukaryotic cells are under continuous assault by cosmic and terrestrial radiation sources, as well as the byproducts of the intracellular metabolism such as reactive oxygen species and replication errors that lead to the generation of DNA double strand breaks (DSBs). In addition to accidental events, DSBs are also generated during scheduled biological processes such as meiosis or V(D)J recombination and during diagnostic procedures utilizing ionizing radiation. Finally, therapeutic interventions in the therapy of cancer, such as exposure to ionizing radiation and treatment with certain chemotherapeutic agents, leads to the generation of DSBs. DSBs are severe lesions with a high probability of conversion to lethal or mutagenic events. Therefore, eukaryotic cells have developed efficient repair pathways to remove DSBs from their genome. These repair activities are supported by signaling pathways, termed checkpoints that register the presence of DSBs and arrest or delay cell cycle progression in G1, S, or G2 phase to prevent modifications compromising repair or generating lethal events.

  • Evaluation of the relative contribution of the different pathways implicated in double strand break repair
  • Elucidation of the contribution of checkpoints in double strand break repair
  • Molecular mechanisms of hyperthermia induced checkpoint activation
  • Radiation effects in mouse embryonic tissue with emphasis on genomic instability

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Selected Publications

    Journal articles

  • Wang, Huichen; Perrault, Ange Ronel; Takeda, Yoshihiko; Qin, Wei; Wang, Hongyan; Iliakis, George
    Biochemical evidence for Ku-independent backup pathways of NHEJ
    In: Nucleic Acids Research Vol. 48 (2020) Nr. 9, pp. 5200
    ISSN: 1362-4962; 0301-5610; 0305-1048
  • Wang, Xiang; Guan, Jun; Hu, Baocheng; Weiss, Robert S.; Iliakis, George; Wang, Ya
    Involvement of Hus1 in the chain elongation step of DNA replication after exposure to camptothecin or ionizing radiation
    In: Nucleic Acids Research Vol. 48 (2020) Nr. 6, pp. 3396
    ISSN: 1362-4962; 0301-5610; 0305-1048
  • Mladenov, Emil; Staudt, Christian; Soni, Aashish; Murmann-Konda, Tamara; Siemann-Loekes, Maria; Iliakis, George
    Strong suppression of gene conversion with increasing DNA double-strand break load delimited by 53BP1 and RAD52
    In: Nucleic Acids Research Vol. 48 (2020) Nr. 4, pp. 1905 - 1924
    ISSN: 1362-4962; 0305-1048
  • Iliakis, George; Mladenov, Emil; Mladenova, Veronika
    Necessities in the processing of DNA double strand breaks and their effects on genomic instability and cancer
    In: Cancers Vol. 11 (2019) Nr. 11, pp. 1671
    ISSN: 2072-6694
  • Schipler, Agnes; Mladenova, Veronika; Soni, Aashish; Nikolov, Vladimir; Saha, Janapriya; Mladenov, Emil; Iliakis, George
    Chromosome thripsis by DNA double strand break clusters causes enhanced cell lethality, chromosomal translocations and 53BP1-recruitment
    In: Nucleic Acids Research Vol. 44 (2016) Nr. 16, pp. 7673 - 7690
    ISSN: 1362-4962; 0301-5610; 0305-1048
  • van den Boom, Johannes; Wolf, Markus; Weimann, Lena; Schulze, Nina; Li, Fanghua; Kaschani, Farnusch; Riemer, Anne; Zierhut, Christian; Kaiser, Markus; Iliakis, George; Funabiki, Hironori; Meyer, Hemmo
    VCP/p97 Extracts Sterically Trapped Ku70/80 Rings from DNA in Double-Strand Break Repair
    In: Molecular Cell Vol. 64 (2016) Nr. 1, pp. 189 - 198
    ISSN: 1097-4164; 1097-2765
  • Iliakis, George; Magin, Simon
    Balance shift between error-free and error prone DNA double-strand break repair as a novel mechanism of radiosensitization by nucleoside analogs
    In: Cancer Research Vol. 75 (2015) Nr. Suppl. 15, pp. 3629
    ISSN: 0008-5472; 1538-7445
  • Costantino, L.; Sotiriou, S. K.; Rantala, J. K.; Magin, S.; Mladenov, E.; Helleday, T.; Haber, J. E.; Iliakis, George; Kallioniemi, O. P.; Halazonetis, T. D.
    Break-induced replication repair of damaged forks induces genomic duplications in human cells
    In: Science Vol. 343 (2014) Nr. 6166, pp. 88 - 91
    ISSN: 0036-8075
  • Soni, Aashish; Siemann, Maria; Grabos, Martha; Murmann, Tamara; Pantelias, Gabriel E; Iliakis, George
    Requirement for Parp-1 and DNA ligases 1 or 3 but not of Xrcc1 in chromosomal translocation formation by backup end joining
    In: Nucleic Acids Research Vol. 42 (2014) Nr. 10, pp. 6380 - 6392
    ISSN: 1362-4962; 0301-5610; 0305-1048
  • Schipler, Agnes; Iliakis, George
    DNA double-strand-break complexity levels and their possible contributions to the probability for error-prone processing and repair pathway choice
    In: Nucleic Acids Research Vol. 41 (2013) Nr. 16, pp. 7589 - 7605
    ISSN: 1362-4962; 0301-5610; 0305-1048
  • Arakawa, Hiroshi; Bednar, Theresa; Wang, Minli; Paul, Katja; Mladenov, Emil; Bencsik-Theilen, Alena A.; Iliakis, George
    Functional redundancy between DNA ligases i and III in DNA replication in vertebrate cells
    In: Nucleic Acids Research Vol. 40 (2012) Nr. 6, pp. 2599 - 2610
    ISSN: 1362-4962; 0301-5610; 0305-1048
  • Singh, Satyendra K.; Wang, Minli; Staudt, Christian; Iliakis, George
    Post-irradiation chemical processing of DNA damage generates double-strand breaks in cells already engaged in repair
    In: Nucleic Acids Research Vol. 39 (2011) Nr. 19, pp. 8416 - 8429
    ISSN: 1362-4962; 0301-5610; 0305-1048
  • Klammer, Holger; Kadhim, Munira; Iliakis, George
    Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells
    In: Cancer Research Vol. 70 (2010) Nr. 21, pp. 8498 - 8506
    ISSN: 0008-5472; 1538-7445