ZMB Member Perihan Nalbant

ZMB Member
Perihan Nalbant

Next ZMB-Member
portrait photo of ZMB member Perihan Nalbant
© UDE/Nalbant

Prof. Dr. Perihan Nalbant

Faculty of Biology
University of Duisburg-Essen
Universitätsstr. 2
45141 Essen

Research Overview

A wide range of molecules has been shown to be important for cellular migration. Unfortunately, in cancer, many regulatory processes are aberrant and lead to highly invasive cell behavior and metastasis. In particular, the proteins of the Rho GTPase family, RhoA, Cdc42 and Rac, are most prominently known to be crucial for cell migration and other processes related to cancer invasion. The focus of our research is to understand how spatial and temporal regulation of Rho GTPase activity is organized during cellular migration and how such mechanisms are altered in cancer.
To achieve this, we are using fluorescence biosensor imaging to visualize localized GTPase activity in living cells. The combination of these tools with other contemporary cell biological methods such as siRNA technology is a powerful means to dissect how the complex network of upstream regulators affects localized Rho protein signaling and how this is translated into normal as well as aberrant cell migration.
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Selected Publications

    Journal articles

  • Kowalczyk, Manuela; Kamps, Dominic; Wu, Yaowen; Dehmelt, Leif; Nalbant, Perihan
    Monitoring the Response of Multiple Signal Network Components to Acute Chemo-Optogenetic Perturbations in Living Cells
    In: ChemBioChem Vol. 23 (2022) Nr. 4,
    ISSN: 1439-7633; 1439-4227
  • Dorsch, Madeleine; Kowalczyk, Manuela; Planque, Mélanie; Heilmann, Geronimo; Urban, Sebastian; Dujardin, Philip; Forster, Jan; Ueffing, Kristina; Nothdurft, Silke; Oeck, Sebastian; Paul, Annika; Liffers, Sven T.; Kaschani, Farnusch; Kaiser, Markus; Schramm, Alexander; Siveke, Jens; Winslow, Monte M.; Fendt, Sarah-Maria; Nalbant, Perihan; Grüner, Barbara M.
    Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis
    In: Cell Reports Vol. 37 (2021) Nr. 8,
    ISSN: 2211-1247
  • Kamps, Dominic; Koch, Johannes; Juma, Victor O.; Campillo-Funollet, Eduard; Gräßl, Melanie; Banerjee, Soumya; Mazel, Tomáš; Chen, Xi; Wu, Yao-Wen; Portet, Stephanie; Madzvamuse, Anotida; Nalbant, Perihan; Dehmelt, Leif
    Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network
    In: Cell Reports Vol. 33 (2020) Nr. 9, pp. 108467
    ISSN: 2211-1247
  • Nalbant, Perihan; Dehmelt, Leif
    Exploratory cell dynamics : A sense of touch for cells?
    In: Biological Chemistry Vol. 399 (2018) Nr. 8, pp. 809 - 819
    ISSN: 1437-4315; 1431-6730
  • Gräßl, Melanie; Koch, Johannes; Calderon, Abram; Kamps, Dominic; Banerjee, Soumya; Mazel, Tomáš; Schulze, Nina; Jungkurth, Jana; Patwardhan, Rutuja; Solouk, Djamschid; Hampe, Nico; Hoffmann, Bernd; Dehmelt, Leif; Nalbant, Perihan
    An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
    In: The Journal of Cell Biology (JCB) Vol. 216 (2017) Nr. 12, pp. 4271 - 4285
    ISSN: 1540-8140; 0021-9525
  • Jeruschke, Stefanie; Jeruschke, Kay; DiStasio, Andrew; Karaterzi, Sinem; Büscher (A.), Anja; Nalbant, Perihan; Klein-Hitpaß, Ludger; Hoyer, Peter Friedrich; Weiss, Jürgen; Stottmann, Rolf W; Weber, Stefanie
    Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression
    In: PLoS ONE Vol. 10 (2015) Nr. 9, pp. e0137043
    ISSN: 1932-6203
  • Jeruschke, Stefanie; Rekasi, Heike; Karaterzi, Sinem; Buescher, AnjaKarin; Klein-Hitpaß, Ludger; Jeruschke, Kay; Weiss, Juergen; Saleem, MoinAhson; Hoyer, Peter Friedrich; Nalbant, Perihan; Weber, Stefanie
    Everolimus Prevents Podocyte Injury Via Stabilizing Tubb2b And Dcdc2 Expression
    In: Pediatric Nephrology Vol. 29 (2014) Nr. 9, pp. 1674 - 1674
    ISSN: 0931-041X
  • Schulze, Nina; Gräßl, Melanie; Soares, Alexandra Blancke; Geyer, Matthias; Dehmelt, Leif; Nalbant, Perihan
    FHOD1 regulates stress fiber organization by controlling the dynamics of transverse arcs and dorsal fibers
    In: Journal of Cell Science Vol. 127 (2014) Nr. 7, pp. 1379 - 1393
    ISSN: 0021-9533; 1477-9137; 0370-2952
  • Jeruschke, S.; Büscher (A.), Anja; Rekasi, H.; Karaterzi, S.; Saleem, M. A.; Oh, J.; Hoyer, Peter Friedrich; Nalbant, Perihan; Weber, Stefanie
    Differential expression of cytoskeletal-associated genes in human podocytes by the mTOR inhibitor everolimus
    In: Pediatric Nephrology Vol. 28 (2013) Nr. 8, pp. 1438 - 1438
    ISSN: 0931-041X
  • Jeruschke, Stefanie; Büscher, Anja Katrin; Oh, Jun; Saleem, Moin Ahson; Hoyer, Peter Friedrich; Weber, Stefanie; Nalbant, Perihan
    Protective effects of the mTOR inhibitor everolimus on cytoskeletal injury in human podocytes are mediated by RhoA signaling
    In: PLoS ONE Vol. 8 (2013) Nr. 2, pp. e55980
    ISSN: 1932-6203
  • Sokolova, Viktoriya; Rotan, Olga; Klesing, Jan; Nalbant, Perihan; Buer, Jan; Knuschke, Torben; Westendorf, Astrid; Epple, Matthias
    Calcium phosphate nanoparticles as versatile carrier for small and large molecules across cell membranes
    In: Journal of Nanoparticle Research Vol. 14 (2012) Nr. 6,
    ISSN: 1388-0764; 1572-896X
  • Jeruschke, Stefanie; Büscher (A.), Anja; Saleem, Moin; Hoyer, Peter Friedrich; Oh, Jun; Nalbant, Perihan; Weber, Stefanie
    Protective effects of everolimus on puromycin-induced cytoskeletal alterations in human podocytes are mediated by RhoA-signalling
    In: Pediatric Nephrology Vol. 27 (2012) Nr. 9, pp. 1638 - 1638
    ISSN: 0931-041X
  • MacHacek, Matthias; Hodgson, Louis; Welch, Christopher; Elliott, Hunter; Pertz, Oliver; Nalbant, Perihan; Abell, Amy; Johnson, Gary L.; Hahn, Klaus M.; Danuser, Gaudenz
    Coordination of Rho GTPase activities during cell protrusion
    In: Nature Vol. 461 (2009) Nr. 7260, pp. 99 - 103
    ISSN: 0028-0836; 1476-4687
  • Nalbant, Perihan; Chang, Y.-C.; Birkenfeld, J.; Chang, Z.-F.; Bokoch, G.M.
    Guanine nucleotide exchange factor-H1 regulates cell migration via localized activation of RhoA at the leading edge
    In: Molecular Biology of the Cell Vol. 20 (2009) Nr. 18, pp. 4070 - 4082
  • Manukyan, Maria; Nalbant, Perihan; Luxen, Sylvia; Hahn, Klaus M.; Knaus, Ulla G.
    RhoA GTPase activation by TLR2 and TLR3 ligands: Connecting via Src to NF-κB
    In: The Journal of Immunology Vol. 182 (2009) Nr. 6, pp. 3522 - 3529
    ISSN: 0022-1767
  • Chang, Y.-C.; Nalbant, Perihan; Birkenfeld, J.; Chang, Z.-F.; Bokoch, G.M.
    3GEF-H1 couples nocodazole-induced microtubule disassembly to cell contractility via RhoA
    In: Molecular Biology of the Cell Vol. 19 (2008) Nr. 5, pp. 2147 - 2153
    ISSN: 1059-1524
  • Birkenfeld, Jörg; Nalbant, Perihan; Yoon, Soon-Hee; Bokoch, Gary M.
    Cellular functions of GEF-H1, a microtubule-regulated Rho-GEF: is altered GEF-H1 activity a crucial determinant of disease pathogenesis?
    In: Trends in Cell Biology Vol. 18 (2008) Nr. 5, pp. 210 - 219
    ISSN: 0962-8924
  • Carlile, Mark; Nalbant, Perihan; Preston-Fayers, Keziah; McHaffie, Gavin S.; Werner, Andreas
    Processing of naturally occurring sense/antisense transcripts of the vertebrate Slc34a gene into short RNAs
    In: Physiological Genomics Vol. 34 (2008) Nr. 1, pp. 95 - 100
    ISSN: 1094-8341; 1531-2267
  • Lim, Yangmi; Lim, Ssang-Taek; Tomar, Alok; Gardel, Margaret; Bernard-Trifilo, Joie A.; Xiao, Lei Chen; Uryu, Sean A.; Canete-Soler, Rafaela; Zha, Jinbin; Lin, Hong; Schlaepfer, William W.; Nalbant, Perihan; Bokoch, Gary; Ilic, Dusko; Waterman-Storer, Clare; Schlaepfer, David D.
    PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility
    In: The Journal of Cell Biology (JCB) Vol. 180 (2008) Nr. 1, pp. 187 - 203
    ISSN: 0021-9525; 1540-8140
  • El-Sibai, Mirvat; Nalbant, Perihan; Pang, Huan; Flinn, Rory J.; Sarmiento, Corina; Macaluso, Frank; Cammer, Michael; Condeelis, John S.; Hahn, Klaus M.; Backer, Jonathan M.
    Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells
    In: Journal of Cell Science Vol. 120 (2007) Nr. 19, pp. 3465 - 3474
    ISSN: 0021-9533; 1477-9137; 0370-2952
  • Birkenfeld, Jörg; Nalbant, Perihan; Bohl, Benjamin P.; Pertz, Oliver; Hahn, Klaus M.; Bokoch, Gary M.
    GEF-H1 Modulates Localized RhoA Activation during Cytokinesis under the Control of Mitotic Kinases
    In: Developmental Cell Vol. 12 (2007) Nr. 5, pp. 699 - 712
    ISSN: 1534-5807; 1878-1551
  • Frantz, Christian; Karydis, Anastasios; Nalbant, Perihan; Hahn, Klaus M.; Barber, Diane L.
    Positive feedback between Cdc42 activity and H+ efflux by the Na-H exchanger NHE1 for polarity of migrating cells
    In: Journal of Cell Biology Vol. 179 (2007) Nr. 3, pp. 403 - 410
    ISSN: 0021-9525
  • Zhao, Tieming; Nalbant, Perihan; Hoshino, Mikio; Dong, Xuemei; Wu, Dianqing; Bokoch, Gary M.
    Signaling requirements for translocation of P-Rex1, a key Rac2 exchange factor involved in chemoattractant-stimulated human neutrophil function
    In: Journal of Leukocyte Biology Vol. 81 (2007) Nr. 4, pp. 1127 - 1136
    ISSN: 0741-5400
  • Manukyan, Maria; Nalbant, Perihan; Pertz, Oliver; Halut, Klaus; Knaus, Ulla G.
    Visualizing TLR2- and TLR3-dependent RhoA activation by fret biosensor
    In: Journal of Leukocyte Biology (2007) Nr. Supplement, pp. 36 - 36
    ISSN: 0741-5400; 1938-3673
  • Dehmelt, Leif; Nalbant, Perihan; Steffen, Walter; Halpain, Shelley
    A microtubule-based, dynein-dependent force induces local cell protrusions: Implications for neurite initiation
    In: Brain Cell Biology Vol. 35 (2006) Nr. 1, pp. 39 - 56
    ISSN: 1559-7105
  • Hodgson, Louis; Nalbant, Perihan; Shen, Feimo; Hahn, Klaus M.
    Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation
    In: Methods in Enzymology Vol. 406 (2006) Nr. 12, pp. 140 - 156
    ISSN: 0076-6879; 1557-7988
  • Belvindrah, R.; Nalbant, Perihan; Ding, S.; Wu, C.; Bokoch, Gary M.; Müller, U.
    Integrin-linked kinase regulates Bergmann glial differentiation during cerebellar development
    In: Molecular and Cellular Neuroscience (MCN) Vol. 33 (2006) Nr. 2, pp. 109 - 125
    ISSN: 1044-7431; 1095-9327
  • Subauste, M. Cecilia; Nalbant, Perihan; Adamson, Eileen D.; Hahn, Klaus M.
    Vinculin controls PTEN protein level by maintaining the interaction of the adherens junction protein β-catenin with the scaffolding protein MAGI-2
    In: The Journal of Biological Chemistry (JBC) Vol. 280 (2005) Nr. 7, pp. 5676 - 5681
    ISSN: 0021-9258; 1083-351X
  • Nalbant, Perihan; Hodgson, Louis; Kraynov, Vadim; Toutchkine, Alexei; Hahn, Klaus M.
    Activation of endogenous Cdc42 visualized in living cells
    In: Science Vol. 305 (2004) Nr. 5690, pp. 1615 - 1619
    ISSN: 0036-8075
  • Tan, Xin; Nalbant, Perihan; Toutchkine, Alexei; Hu, Dehong; Vorpagel, Erich R.; Hahn, Klaus M.; Lu, H. Peter
    Single-Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry Vol. 108 (2004) Nr. 2, pp. 737 - 744
    ISSN: 1520-6106; 1089-5647; 1520-5207
  • Graham, C.; Nalbant, Perihan; Schölermann, B.; Hentschel, H.; Kinne, R. K. H.; Werner, A.
    Characterization of a type IIb sodium-phosphate cotransporter from zebrafish (Danio rerio) kidney
    In: American Journal of Physiology - Renal Physiology Vol. 284 (2003) Nr. 4, pp. F727 - F736
    ISSN: 0363-6127
  • Tan, Xin; Nalbant, Perihan; Hu, Dehong; Vorpagel, Erich R.; Hahn, Klaus M.; Lu, H. Peter
    Single-molecule protein-protein interaction dynamics of GTP-binding protein Cdc42 with downstream effector WASP
    In: Molecular Biology of the Cell Vol. 84 (2003) Nr. Suppl 2 Pt 2, pp. 485a
    ISSN: 0006-3495
  • Toutchkine, Alexei; Nalbant, Perihan; Hahn, Klaus M.
    Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility
    In: Bioconjugate Chemistry Vol. 13 (2002) Nr. 3, pp. 387 - 391
    ISSN: 1043-1802; 1520-4812
  • Werner, A.; Preston-Fayers, K.; Dehmelt, L.; Nalbant, Perihan
    Regulation of the NPT gene by a naturally occurring antisense transcript
    In: Cell Biochemistry and Biophysics Vol. 36 (2002) Nr. 2/3, pp. 241 - 252
    ISSN: 1085-9195
  • Nalbant, Perihan; Boehmer, Christoph; Dehmelt, Leif; Wehner, Frank; Werner, Andreas
    Functional characterization of a Na+-phosphate cotransporter (NaP(i)-II) from zebrafish and identification of related transcripts
    In: Journal of Physiology Vol. 520 (1999) Nr. 1, pp. 79 - 89
    ISSN: 0022-3751
  • Werner, Andreas; Dehmelt, Leif; Nalbant, Perihan
    Na+-dependent phosphate cotransporters : The NaPi protein families
    In: The Journal of Experimental Biology Vol. 201 (1998) Nr. 23, pp. 3135 - 3142
    ISSN: 0022-0949; 1477-9145