ZMB Member Shirley Knauer

ZMB Member
Shirley Knauer

Next ZMB-Member
portrait Shirley Knauer
© Knauer

Prof. Dr. Shirley Knauer

Group

Faculty of Biology
University Duisburg-Essen
45141 Essen

Research Overview

Our research interests focus on molecular and translational clinical oncology as well as on the biomedical transfer of the compiled results. They aim on a detailed understanding of the regulation of nucleo-cytoplasmic transport processes, their impact on malignant transformation in cancer development, the discovery of novel therapeutic targets as well as the establishment of novel drug-screening assays.

Current projects concentrate on the molecular and pathophysiological function of the "Inhibitor of Apoptosis" protein Survivin, which is attributed a decisive role in the development of cancer and other diseases.

Using corroborating experimental approaches including microinjection experiments, flourescence as well as confocal microscopy, we could demonstrate that Survivin is actively exported from the cell nucleus. This transport process was not only a prerequisite for protection of cancer cells against radio- and chemotherapy by Survivin, but also plays a causal role in the treatment response of cancer patients. Therefore, targeted interference with the nuclear export of survivin by molecular decoys seems to be a highly promising innovative strategy to impede growth and therapy resistance of cancer cells. Additionally, the detailed functional characterisation of the anti-apoptotic protein Survivin and its cellular transport activity led to novel groundbreaking insights concerning the essential involvement of transport receptors in mitotic processes.

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Selected Publications

    Journal articles

  • Gigante, Alba; Sijbesma, Eline; Sánchez-Murcia, Pedro A.; Hu, Xiaoyu; Bier, David; Bäcker, Sandra; Knauer, Shirley; Gago, Federico; Ottmann, Christian; Schmuck, Carsten
    A Supramolecular Stabilizer of the 14-3-3ζ/ERα Protein-Protein Interaction with a Synergistic Mode of Action
    In: Angewandte Chemie International Edition Vol. 132 (2020) Nr. 13, pp. 5322 - 5325
    ISSN: 1521-3773; 1433-7851; 0570-0833
  • Siemer, Svenja; Westmeier, Dana; Barz, Matthias; Eckrich, Jonas; Wünsch, Désirée; Seckert, Christof; Thyssen, Christian; Schilling, Oliver; Hasenberg, Mike; Pang, Chengfang; Docter, Dominic; Knauer, Shirley K.; Stauber, Roland H.; Strieth, Sebastian
    Corrigendum to “Biomolecule-corona formation confers resistance of bacteria to nanoparticle-induced killing: Implications for the design of improved nanoantibiotics” [Biomaterials 192 (2019) 551–559]
    In: Biomaterials Vol. 263 (2020) pp. 120371
    ISSN: 1878-5905; 0142-9612
  • Vallet, Cecilia; Aschmann, Dennis; Beuck, Christine; Killa, Matthias; Meiners, Annika; Mertel, Marcel; Ehlers, Martin; Bayer, Peter; Schmuck, Carsten; Giese, Michael R. A.; Knauer, Shirley
    Functional Disruption of the Cancer-Relevant Interaction between Survivin and Histone H3 with a Guanidiniocarbonyl Pyrrole Ligand
    In: Angewandte Chemie International Edition Vol. 59 (2020) Nr. 14, pp. 5567 - 5571
    ISSN: 1521-3773; 1433-7851; 0570-0833
  • Siemer, Svenja; Westmeier, Dana; Barz, Matthias; Eckrich, Jonas; Wünsch, Désirée; Seckert, Christof; Thyssen, Christian; Schilling, Oliver; Hasenberg, Mike; Pang, Chengfan; Docter, Dominic; Knauer, Shirley K.; Stauber, Roland H.; Strieth, Sebastian
    Biomolecule-corona formation confers resistance of bacteria to nanoparticle-induced killing : Implications for the design of improved nanoantibiotics
    In: Biomaterials Vol. 192 (2019) pp. 551 - 559
    ISSN: 0142-9612; 1878-5905
  • Westmeier, Dana; Hahlbrock, Angelina; Reinhardt, Christoph; Fröhlich-Nowoisky, Janine; Wessler, Silja; Vallet, Cecilia; Pöschl, Ulrich; Knauer, Shirley; Stauber, Roland H.
    Nanomaterial-microbe cross-talk : physicochemical principles and (patho)biological consequences
    In: Chemical Society Reviews Vol. 47 (2018) Nr. 14, pp. 5312 - 5337
    ISSN: 1460-4744; 0306-0012
  • Westmeier, Dana; Solouk-Saran, Djamschid; Vallet, Cecilia; Siemer, Svenja; Docter, Dominic; Götz, Hermann; Männ, Linda; Hasenberg, Anja; Hahlbrock, Angelina; Erler, Kathrin; Reinhardt, Christoph; Schilling, Oliver; Becker, Sven; Gunzer, Matthias; Hasenberg, Mike; Knauer, Shirley; Stauber, Roland H.
    Nanoparticle decoration impacts airborne fungal pathobiology
    In: Proceedings of the National Academy of Sciences of the United States of America Vol. 115 (2018) Nr. 27, pp. 7087 - 7092
    ISSN: 1091-6490; 0027-8424
  • Jana, Poulami; Samanta, Krishnananda; Bäcker, Sandra; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles
    In: Angewandte Chemie International Edition Vol. 56 (2017) Nr. 28, pp. 8083 - 8088
    ISSN: 1521-3773; 1433-7851
  • Jiang, Hao; Hu, Xiao-Yu; Schlesiger, Stefanie; Li, Mao; Zellermann, Eilo; Knauer, Shirley; Schmuck, Carsten
    Morphology-Dependent Cell Imaging by Using a Self-Assembled Diacetylene Peptide Amphiphile
    In: Angewandte Chemie International Edition Vol. 56 (2017) Nr. 46, pp. 14526 - 14530
    ISSN: 1521-3773; 1433-7851
  • Li, Mao; Ehlers, Martin; Schlesiger, Stefanie; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Incorporation of a Non-Natural Arginine Analogue into a Cyclic Peptide Leads to Formation of Positively Charged Nanofibers Capable of Gene Transfection
    In: Angewandte Chemie International Edition Vol. 55 (2016) Nr. 2, pp. 598 - 601
    ISSN: 1521-3773; 1433-7851
  • Li, Mao; Schlesiger, Stefanie; Knauer, Shirley; Schmuck, Carsten
    A Tailor-Made Specific Anion-Binding Motif in the Side Chain Transforms a Tetrapeptide into an Efficient Vector for Gene Delivery
    In: Angewandte Chemie International Edition Vol. 54 (2015) Nr. 10, pp. 2941 - 2944
    ISSN: 1433-7851; 0044-8249; 0570-0833
  • Docter, Dominic; Westmeier, Dana; Markiewicz, Marta; Stolte, Stefan; Knauer, Shirley; Stauber, Roland H.
    The nanoparticle biomolecule corona : lessons learned – challenge accepted?
    In: Chemical Society Reviews Vol. 44 (2015) Nr. 17, pp. 6094 - 6121
    ISSN: 1460-4744; 0306-0012
  • Docter, Dominic; Distler, Ute; Storck, Wiebke; Kuharev, Jörg; Wünsch, Desirée; Hahlbrock, Angelina; Knauer, Shirley K.; Tenzer, Stefan; Stauber, Roland H.
    Quantitative profiling of the protein coronas that form around nanoparticles
    In: Nature Protocols Vol. 9 (2014) Nr. 9, pp. 2030 - 2044
    ISSN: 1750-2799; 1754-2189
  • Tenzer, Stefan; Docter, Dominic; Kuharev, Jörg; Musyanovych, Anna; Fetz, Verena; Hecht, Rouven; Schlenk, Florian; Fischer, Dagmar; Kiouptsi, Klytaimnistra; Reinhardt, Christoph; Landfester, Katharina; Schild, Hansjörg; Maskos, Michael; Knauer, Shirley; Stauber, Roland H.
    Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
    In: Nature Nanotechnology Vol. 8 (2013) Nr. 10, pp. 772 - 781
    ISSN: 1748-3395
  • Krämer, Oliver H.; Stauber, Roland H.; Bug, Gesine; Hartkamp, Jörg; Knauer, Shirley
    SIAH proteins : Critical roles in leukemogenesis
    In: Leukemia Vol. 27 (2013) Nr. 4, pp. 792 - 802
    ISSN: 0887-6924; 1476-5551
  • Kuchelmeister, Hannes Y.; Karczewski (ehem.Tillmann), Sarah; Gutschmidt, Aljona; Knauer, Shirley; Schmuck, Carsten
    Utilizing Combinatorial Chemistry and Rational Design : Peptidic Tweezers with Nanomolar Affinity to DNA Can Be Transformed into Efficient Vectors for Gene Delivery by Addition of a Lipophilic Tail
    In: Angewandte Chemie International Edition Vol. 52 (2013) Nr. 52, pp. 14016 - 14020
    ISSN: 0570-0833; 1521-3773
  • Pless, Birgit; Oehm, Clarissa; Knauer, Shirley; Stauber, Roland; Dingermann, Theo; Marschalek, Rolf
    The heterodimerization domains of MLL-FYRN and FYRC-are potential target structures in t(4;11) leukemia.
    In: Leukemia Vol. 25 (2011) Nr. 4, pp. 663 - 670
    ISSN: 0887-6924
  • Knauer, Shirley; Bier, Carolin; Habtemichael, Negusse; Stauber, Roland H.
    The Survivin-Crm1 interaction is essential for chromosomal passenger complex localization and function.
    In: EMBO Reports Vol. 7 (2006) Nr. 12, pp. 1259 - 1265
    ISSN: 1469-221X