ZMB Member Shirley Knauer

ZMB Member
Shirley Knauer

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portrait Shirley Knauer
© Knauer

Prof. Dr. Shirley Knauer

Group

Molecular Biology II

Faculty of Biology
University Duisburg-Essen
45141 Essen

Research Overview

In our department Molecular Biology II we follow a competitive interdisciplinary research line at the interface of basic and translational molecular biology. Building on our technological and scientific expertise in unravelling molecular mechanisms of (cancer) cell biology, nucleocytoplasmic transport and cell-based assays, the department focuses on the topic: 'Cell fate decisions in development and disease'. Our group’s projects are centered around the mechanistic (patho)biological networks of Survivin and Taspase1.

Here, we aim to achieve a detailed understanding of the different biological functions of Survivin in cell stress, including the DNA damage response and cellular senescence. Further, we want to deepen our knowledge on how the protease Taspase1 and its downstream targets contribute to cell fate decisions, and how this might be related to substrate-specific cleavage. As 'innovative tools', we combine our high-end live cell microscopy with cancer-phenotypical and biochemical protein-protein interaction assays, chemicals, nano-based probes, specific nanobodies and targeted protein degradation (TPD) to explore and answer these scientific questions.

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Selected Publications

    Journal articles

  • Stahl, Paul; Kollenda, Sebastian; Sager, Jonas; Schmidt, Laura; Schroer, Martin; Stauber, Roland H.; Epple, Matthias; Knauer, Shirley
    Tuning Nanobodies’ Bioactivity : Coupling to Ultrasmall Gold Nanoparticles Allows the Intracellular Interference with Survivin
    In: Small Vol. 19 (2023) Nr. 33, 2300871 10.1002/smll.202300871
    Online Full Text: dx.doi.org/ (Open Access)
  • Rick, Tim; Kreiling, Vanessa; Höing, Alexander; Fiedler, Svenja; Glatter, Timo; Steinchen, Wieland; Hochberg, Georg; Bähre, Heike; Seifert, Roland; Bange, Gert; Knauer, Shirley K.; Graumann, Peter L.; Thormann, Kai M.; Roland, Seifert
    GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP
    In: npj Biofilms and Microbiomes Vol. 8 (2022) Nr. 1, 35
    Online Full Text: dx.doi.org/ (Open Access)
  • Hensel, Astrid; Stahl, Paul; Moews, Lisa; König, Lena; Patwardhan, Rutuja; Höing, Alexander; Schulze, Nina; Nalbant, Perihan; Stauber, Roland H.; Knauer, Shirley
    The Taspase1/Myosin1f-axis regulates filopodia dynamics
    In: iScience Vol. 25 (2022) Nr. 6, 104355
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Meiners, Annika; Bäcker, Sandra; Hadrovic, Inesa; Heid, Christian; Beuck, Christine; Ruiz-Blanco, Yasser B.; Mieres-Perez, Joel; Pörschke, Marius; Grad, Jean-Noël; Vallet, Cecilia; Hoffmann, Daniel; Bayer, Peter; Sánchez-García, Elsa; Schrader, Thomas; Knauer, Shirley
    Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
    In: Nature Communications Vol. 12 (2021) Nr. 1, pp. 1505
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Gigante, Alba; Sijbesma, Eline; Sánchez-Murcia, Pedro A.; Hu, Xiaoyu; Bier, David; Bäcker, Sandra; Knauer, Shirley; Gago, Federico; Ottmann, Christian; Schmuck, Carsten
    A Supramolecular Stabilizer of the 14-3-3ζ/ERα Protein-Protein Interaction with a Synergistic Mode of Action
    In: Angewandte Chemie International Edition Vol. 132 (2020) Nr. 13, pp. 5322 - 5325
    Online Full Text: dx.doi.org/ (Open Access)
  • Westmeier, Dana; Posselt, Gernot; Hahlbrock, Angelina; Bartfeld, Sina; Vallet, Cecilia; Abfalter, Carmen; Docter, Dominic; Knauer, Shirley; Wessler, Silja; Stauber, Roland H.
    Erratum : Nanoparticle binding attenuates the pathobiology of gastric cancer-associated: Helicobacter pylori
    In: Nanoscale Vol. 12 (2020) Nr. 3, pp. 2154 - 2155
    Online Full Text: dx.doi.org/ (Open Access)
  • Vallet, Cecilia; Aschmann, Dennis; Beuck, Christine; Killa, Matthias; Meiners, Annika; Mertel, Marcel; Ehlers, Martin; Bayer, Peter; Schmuck, Carsten; Giese, Michael R. A.; Knauer, Shirley
    Functional Disruption of the Cancer-Relevant Interaction between Survivin and Histone H3 with a Guanidiniocarbonyl Pyrrole Ligand
    In: Angewandte Chemie International Edition Vol. 59 (2020) Nr. 14, pp. 5567 - 5571
    Online Full Text: dx.doi.org/ (Open Access)
  • van den Boom, Johannes; Hensel, Astrid; Trusch, Franziska; Matena, Anja; Siemer, Svenja; Guel, Désirée; Docter, Dominic; Höing, Alexander; Bayer, Peter; Stauber, Roland H.; Knauer, Shirley
    The other side of the corona : Nanoparticles inhibit the protease taspase1 in a size-dependent manner
    In: Nanoscale Vol. 12 (2020) Nr. 37, pp. 19093 - 19103
    Online Full Text: dx.doi.org/
  • Siemer, Svenja; Westmeier, Dana; Barz, Matthias; Eckrich, Jonas; Wünsch, Désirée; Seckert, Christof; Thyssen, Christian; Schilling, Oliver; Hasenberg, Mike; Pang, Chengfan; Docter, Dominic; Knauer, Shirley K.; Stauber, Roland H.; Strieth, Sebastian
    Biomolecule-corona formation confers resistance of bacteria to nanoparticle-induced killing : Implications for the design of improved nanoantibiotics
    In: Biomaterials Vol. 192 (2019) pp. 551 - 559
    Online Full Text: dx.doi.org/
  • Hu, Xiao-Yu; Gao, Lei; Mosel, Stefanie; Ehlers, Martin; Zellermann, Elio; Jiang, Hao; Knauer, Shirley; Wang, Leyong; Schmuck, Carsten
    From Supramolecular Vesicles to Micelles : Controllable Construction of Tumor-Targeting Nanocarriers Based on Host–Guest Interaction between a Pillar[5]arene-Based Prodrug and a RGD-Sulfonate Guest
    In: Small Vol. 14 (2018) Nr. 52, pp. 1803952
    Online Full Text: dx.doi.org/
  • Westmeier, Dana; Hahlbrock, Angelina; Reinhardt, Christoph; Fröhlich-Nowoisky, Janine; Wessler, Silja; Vallet, Cecilia; Pöschl, Ulrich; Knauer, Shirley; Stauber, Roland H.
    Nanomaterial-microbe cross-talk : physicochemical principles and (patho)biological consequences
    In: Chemical Society Reviews Vol. 47 (2018) Nr. 14, pp. 5312 - 5337
    Online Full Text: dx.doi.org/
  • Abfalter, Carmen; Docter, Dominic; Wessler, Silja; Vallet, Cecilia; Westmeier, Dana; Knauer, Shirley; Posselt, Gernot; Stauber, Roland H.; Hahlbrock, Angelina; Bartfeld, Sina
    Nanoparticle binding attenuates the pathobiology of gastric cancer-associated Helicobacter pylori
    In: Nanoscale Vol. 10 (2018) Nr. 3, pp. 1453 - 1463
    Online Full Text: dx.doi.org/
  • Jana, Poulami; Samanta, Krishnananda; Bäcker, Sandra; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles
    In: Angewandte Chemie International Edition Vol. 56 (2017) Nr. 28, pp. 8083 - 8088
    Online Full Text: dx.doi.org/
  • Jiang, Hao; Hu, Xiao-Yu; Schlesiger, Stefanie; Li, Mao; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Morphology-Dependent Cell Imaging by Using a Self-Assembled Diacetylene Peptide Amphiphile
    In: Angewandte Chemie International Edition Vol. 56 (2017) Nr. 46, pp. 14526 - 14530
    Online Full Text: dx.doi.org/
  • Wissler, Jörg; Bäcker, Sandra; Feis, Alessandro; Knauer, Shirley; Schlücker, Sebastian
    Site-Specific SERS Assay for Survivin Protein Dimer : From Ensemble Experiments to Correlative Single-Particle Imaging
    In: Small Vol. 13 (2017) Nr. 32, pp. 1700802
    Online Full Text: dx.doi.org/
  • Li, Mao; Ehlers, Martin; Schlesiger, Stefanie; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Incorporation of a Non-Natural Arginine Analogue into a Cyclic Peptide Leads to Formation of Positively Charged Nanofibers Capable of Gene Transfection
    In: Angewandte Chemie International Edition Vol. 55 (2016) Nr. 2, pp. 598 - 601
    Online Full Text: dx.doi.org/
  • Li, Mao; Schlesiger, Stefanie; Knauer, Shirley; Schmuck, Carsten
    A Tailor-Made Specific Anion-Binding Motif in the Side Chain Transforms a Tetrapeptide into an Efficient Vector for Gene Delivery
    In: Angewandte Chemie International Edition Vol. 54 (2015) Nr. 10, pp. 2941 - 2944
    Online Full Text: dx.doi.org/
  • Docter, Dominic; Westmeier, Dana; Markiewicz, Marta; Stolte, Stefan; Knauer, Shirley; Stauber, Roland H.
    The nanoparticle biomolecule corona : lessons learned – challenge accepted?
    In: Chemical Society Reviews Vol. 44 (2015) Nr. 17, pp. 6094 - 6121
    Online Full Text: dx.doi.org/ (Open Access)
  • Docter, Dominic; Distler, Ute; Storck, Wiebke; Kuharev, Jörg; Wünsch, Desirée; Hahlbrock, Angelina; Knauer, Shirley K.; Tenzer, Stefan; Stauber, Roland H.
    Quantitative profiling of the protein coronas that form around nanoparticles
    In: Nature Protocols Vol. 9 (2014) Nr. 9, pp. 2030 - 2044
    Online Full Text: dx.doi.org/
  • Tenzer, Stefan; Docter, Dominic; Kuharev, Jörg; Musyanovych, Anna; Fetz, Verena; Hecht, Rouven; Schlenk, Florian; Fischer, Dagmar; Kiouptsi, Klytaimnistra; Reinhardt, Christoph; Landfester, Katharina; Schild, Hansjörg; Maskos, Michael; Knauer, Shirley; Stauber, Roland H.
    Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
    In: Nature Nanotechnology Vol. 8 (2013) Nr. 10, pp. 772 - 781
    Online Full Text: dx.doi.org/
  • Krämer, Oliver H.; Stauber, Roland H.; Bug, Gesine; Hartkamp, Jörg; Knauer, Shirley
    SIAH proteins : Critical roles in leukemogenesis
    In: Leukemia Vol. 27 (2013) Nr. 4, pp. 792 - 802
    Online Full Text: dx.doi.org/
  • Kuchelmeister, Hannes Y.; Karczewski, Sarah; Gutschmidt, Aljona; Knauer, Shirley; Schmuck, Carsten
    Utilizing Combinatorial Chemistry and Rational Design : Peptidic Tweezers with Nanomolar Affinity to DNA Can Be Transformed into Efficient Vectors for Gene Delivery by Addition of a Lipophilic Tail
    In: Angewandte Chemie International Edition Vol. 52 (2013) Nr. 52, pp. 14016 - 14020
    Online Full Text: dx.doi.org/
  • Pless, Birgit; Oehm, Clarissa; Knauer, Shirley; Stauber, Roland; Dingermann, Theo; Marschalek, Rolf
    The heterodimerization domains of MLL-FYRN and FYRC-are potential target structures in t(4;11) leukemia.
    In: Leukemia Vol. 25 (2011) Nr. 4, pp. 663 - 670
    Online Full Text: dx.doi.org/
  • Knauer, Shirley; Bier, Carolin; Habtemichael, Negusse; Stauber, Roland H.
    The Survivin-Crm1 interaction is essential for chromosomal passenger complex localization and function.
    In: EMBO Reports Vol. 7 (2006) Nr. 12, pp. 1259 - 1265
    Online Full Text
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