ZMB Member Shirley Knauer

ZMB Member
Shirley Knauer

Next ZMB-Member
portrait Shirley Knauer
© Knauer

Prof. Dr. Shirley Knauer

Group

Molecular Biology II

Faculty of Biology
University Duisburg-Essen
45141 Essen

Research Overview

In our department Molecular Biology II we follow a competitive interdisciplinary research line at the interface of basic and translational molecular biology. Building on our technological and scientific expertise in unravelling molecular mechanisms of (cancer) cell biology, nucleocytoplasmic transport and cell-based assays, the department focuses on the topic: 'Cell fate decisions in development and disease'. Our group’s projects are centered around the mechanistic (patho)biological networks of Survivin and Taspase1.

Here, we aim to achieve a detailed understanding of the different biological functions of Survivin in cell stress, including the DNA damage response and cellular senescence. Further, we want to deepen our knowledge on how the protease Taspase1 and its downstream targets contribute to cell fate decisions, and how this might be related to substrate-specific cleavage. As 'innovative tools', we combine our high-end live cell microscopy with cancer-phenotypical and biochemical protein-protein interaction assays, chemicals, nano-based probes, specific nanobodies and targeted protein degradation (TPD) to explore and answer these scientific questions.

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Press Releases

Selected Publications

    Journal articles

  • Stahl, Paul; Kollenda, Sebastian; Sager, Jonas; Schmidt, Laura; Schroer, Martin; Stauber, Roland H.; Epple, Matthias; Knauer, Shirley
    Tuning Nanobodies’ Bioactivity : Coupling to Ultrasmall Gold Nanoparticles Allows the Intracellular Interference with Survivin
    In: Small, Vol. 19, 2023, Nr. 33, 2300871 , 10.1002/smll.202300871
    DOI (Open Access)
  • Rick, Tim; Kreiling, Vanessa; Höing, Alexander; Fiedler, Svenja; Glatter, Timo; Steinchen, Wieland; Hochberg, Georg; Bähre, Heike; Seifert, Roland; Bange, Gert; Knauer, Shirley K.; Graumann, Peter L.; Thormann, Kai M.; Roland, Seifert
    GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP
    In: npj Biofilms and Microbiomes, Vol. 8, 2022, Nr. 1, 35
    DOI (Open Access)
  • Hensel, Astrid; Stahl, Paul; Moews, Lisa; König, Lena; Patwardhan, Rutuja; Höing, Alexander; Schulze, Nina; Nalbant, Perihan; Stauber, Roland H.; Knauer, Shirley
    The Taspase1/Myosin1f-axis regulates filopodia dynamics
    In: iScience, Vol. 25, 2022, Nr. 6, 104355
    DOI, Online Full Text (Open Access)
  • Meiners, Annika; Bäcker, Sandra; Hadrovic, Inesa; Heid, Christian; Beuck, Christine; Ruiz-Blanco, Yasser B.; Mieres-Perez, Joel; Pörschke, Marius; Grad, Jean-Noël; Vallet, Cecilia; Hoffmann, Daniel; Bayer, Peter; Sánchez-García, Elsa; Schrader, Thomas; Knauer, Shirley
    Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
    In: Nature Communications, Vol. 12, 2021, Nr. 1, pp. 1505
    DOI, Online Full Text (Open Access)
  • Gigante, Alba; Sijbesma, Eline; Sánchez-Murcia, Pedro A.; Hu, Xiaoyu; Bier, David; Bäcker, Sandra; Knauer, Shirley; Gago, Federico; Ottmann, Christian; Schmuck, Carsten
    A Supramolecular Stabilizer of the 14-3-3ζ/ERα Protein-Protein Interaction with a Synergistic Mode of Action
    In: Angewandte Chemie International Edition, Vol. 132, 2020, Nr. 13, pp. 5322 – 5325
    DOI (Open Access)
  • Westmeier, Dana; Posselt, Gernot; Hahlbrock, Angelina; Bartfeld, Sina; Vallet, Cecilia; Abfalter, Carmen; Docter, Dominic; Knauer, Shirley; Wessler, Silja; Stauber, Roland H.
    Erratum : Nanoparticle binding attenuates the pathobiology of gastric cancer-associated: Helicobacter pylori
    In: Nanoscale, Vol. 12, 2020, Nr. 3, pp. 2154 – 2155
    DOI (Open Access)
  • Vallet, Cecilia; Aschmann, Dennis; Beuck, Christine; Killa, Matthias; Meiners, Annika; Mertel, Marcel; Ehlers, Martin; Bayer, Peter; Schmuck, Carsten; Giese, Michael R. A.; Knauer, Shirley
    Functional Disruption of the Cancer-Relevant Interaction between Survivin and Histone H3 with a Guanidiniocarbonyl Pyrrole Ligand
    In: Angewandte Chemie International Edition, Vol. 59, 2020, Nr. 14, pp. 5567 – 5571
    DOI (Open Access)
  • van den Boom, Johannes; Hensel, Astrid; Trusch, Franziska; Matena, Anja; Siemer, Svenja; Guel, Désirée; Docter, Dominic; Höing, Alexander; Bayer, Peter; Stauber, Roland H.; Knauer, Shirley
    The other side of the corona : Nanoparticles inhibit the protease taspase1 in a size-dependent manner
    In: Nanoscale, Vol. 12, 2020, Nr. 37, pp. 19093 – 19103
    DOI
  • Siemer, Svenja; Westmeier, Dana; Barz, Matthias; Eckrich, Jonas; Wünsch, Désirée; Seckert, Christof; Thyssen, Christian; Schilling, Oliver; Hasenberg, Mike; Pang, Chengfan; Docter, Dominic; Knauer, Shirley K.; Stauber, Roland H.; Strieth, Sebastian
    Biomolecule-corona formation confers resistance of bacteria to nanoparticle-induced killing : Implications for the design of improved nanoantibiotics
    In: Biomaterials, Vol. 192, 2019, pp. 551 – 559
    DOI
  • Hu, Xiao-Yu; Gao, Lei; Mosel, Stefanie; Ehlers, Martin; Zellermann, Elio; Jiang, Hao; Knauer, Shirley; Wang, Leyong; Schmuck, Carsten
    From Supramolecular Vesicles to Micelles : Controllable Construction of Tumor-Targeting Nanocarriers Based on Host–Guest Interaction between a Pillar[5]arene-Based Prodrug and a RGD-Sulfonate Guest
    In: Small, Vol. 14, 2018, Nr. 52, pp. 1803952
    DOI
  • Westmeier, Dana; Hahlbrock, Angelina; Reinhardt, Christoph; Fröhlich-Nowoisky, Janine; Wessler, Silja; Vallet, Cecilia; Pöschl, Ulrich; Knauer, Shirley; Stauber, Roland H.
    Nanomaterial-microbe cross-talk : physicochemical principles and (patho)biological consequences
    In: Chemical Society Reviews, Vol. 47, 2018, Nr. 14, pp. 5312 – 5337
    DOI
  • Abfalter, Carmen; Docter, Dominic; Wessler, Silja; Vallet, Cecilia; Westmeier, Dana; Knauer, Shirley; Posselt, Gernot; Stauber, Roland H.; Hahlbrock, Angelina; Bartfeld, Sina
    Nanoparticle binding attenuates the pathobiology of gastric cancer-associated Helicobacter pylori
    In: Nanoscale, Vol. 10, 2018, Nr. 3, pp. 1453 – 1463
    DOI
  • Jana, Poulami; Samanta, Krishnananda; Bäcker, Sandra; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles
    In: Angewandte Chemie International Edition, Vol. 56, 2017, Nr. 28, pp. 8083 – 8088
    DOI
  • Jiang, Hao; Hu, Xiao-Yu; Schlesiger, Stefanie; Li, Mao; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Morphology-Dependent Cell Imaging by Using a Self-Assembled Diacetylene Peptide Amphiphile
    In: Angewandte Chemie International Edition, Vol. 56, 2017, Nr. 46, pp. 14526 – 14530
    DOI
  • Wissler, Jörg; Bäcker, Sandra; Feis, Alessandro; Knauer, Shirley; Schlücker, Sebastian
    Site-Specific SERS Assay for Survivin Protein Dimer : From Ensemble Experiments to Correlative Single-Particle Imaging
    In: Small, Vol. 13, 2017, Nr. 32, pp. 1700802
    DOI
  • Li, Mao; Ehlers, Martin; Schlesiger, Stefanie; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Incorporation of a Non-Natural Arginine Analogue into a Cyclic Peptide Leads to Formation of Positively Charged Nanofibers Capable of Gene Transfection
    In: Angewandte Chemie International Edition, Vol. 55, 2016, Nr. 2, pp. 598 – 601
    DOI
  • Li, Mao; Schlesiger, Stefanie; Knauer, Shirley; Schmuck, Carsten
    A Tailor-Made Specific Anion-Binding Motif in the Side Chain Transforms a Tetrapeptide into an Efficient Vector for Gene Delivery
    In: Angewandte Chemie International Edition, Vol. 54, 2015, Nr. 10, pp. 2941 – 2944
    DOI
  • Docter, Dominic; Westmeier, Dana; Markiewicz, Marta; Stolte, Stefan; Knauer, Shirley; Stauber, Roland H.
    The nanoparticle biomolecule corona : lessons learned – challenge accepted?
    In: Chemical Society Reviews, Vol. 44, 2015, Nr. 17, pp. 6094 – 6121
    DOI (Open Access)
  • Docter, Dominic; Distler, Ute; Storck, Wiebke; Kuharev, Jörg; Wünsch, Desirée; Hahlbrock, Angelina; Knauer, Shirley K.; Tenzer, Stefan; Stauber, Roland H.
    Quantitative profiling of the protein coronas that form around nanoparticles
    In: Nature Protocols, Vol. 9, 2014, Nr. 9, pp. 2030 – 2044
    DOI
  • Tenzer, Stefan; Docter, Dominic; Kuharev, Jörg; Musyanovych, Anna; Fetz, Verena; Hecht, Rouven; Schlenk, Florian; Fischer, Dagmar; Kiouptsi, Klytaimnistra; Reinhardt, Christoph; Landfester, Katharina; Schild, Hansjörg; Maskos, Michael; Knauer, Shirley; Stauber, Roland H.
    Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
    In: Nature Nanotechnology, Vol. 8, 2013, Nr. 10, pp. 772 – 781
    DOI
  • Krämer, Oliver H.; Stauber, Roland H.; Bug, Gesine; Hartkamp, Jörg; Knauer, Shirley
    SIAH proteins : Critical roles in leukemogenesis
    In: Leukemia, Vol. 27, 2013, Nr. 4, pp. 792 – 802
    DOI
  • Kuchelmeister, Hannes Y.; Karczewski, Sarah; Gutschmidt, Aljona; Knauer, Shirley; Schmuck, Carsten
    Utilizing Combinatorial Chemistry and Rational Design : Peptidic Tweezers with Nanomolar Affinity to DNA Can Be Transformed into Efficient Vectors for Gene Delivery by Addition of a Lipophilic Tail
    In: Angewandte Chemie International Edition, Vol. 52, 2013, Nr. 52, pp. 14016 – 14020
    DOI
  • Pless, Birgit; Oehm, Clarissa; Knauer, Shirley; Stauber, Roland; Dingermann, Theo; Marschalek, Rolf
    The heterodimerization domains of MLL-FYRN and FYRC-are potential target structures in t(4;11) leukemia.
    In: Leukemia, Vol. 25, 2011, Nr. 4, pp. 663 – 670
    DOI
  • Knauer, Shirley; Bier, Carolin; Habtemichael, Negusse; Stauber, Roland H.
    The Survivin-Crm1 interaction is essential for chromosomal passenger complex localization and function.
    In: EMBO Reports, Vol. 7, 2006, Nr. 12, pp. 1259 – 1265
    Online Full Text
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