CRC 1093 - Publications Project B4

Area B: Biological Targets

Publications by Prof. Dr. Christian Ottmann

Project B4:
Modulation of 14-3-3 protein-protein interactions by supramolecular chemistry

This project ended in 2020.

X. Guillory, I. Hadrović, P. J. de Vink, A. Sowislok, L. Brunsveld, T. Schrader, C. Ottmann: Supramolecular Enhancement of a Natural 14-3-3 Protein Ligand. J Am Chem Soc. 2021 Sep 1;143(34):13495-13500. doi: 10.1021/jacs.1c07095.

E. Sijbesma , E. Visser , K. Plitzko, P. Thiel, L.-G. Milroy, M. Kaiser, L. Brunsveld, C. Ottmann: Structure-based evolution of a promiscuous inhibitor to a selective stabilizer of protein-protein interactions. Nat Commun. 2020 Aug 7;11(1): 3954.doi: 10.1038/s41467-020-17741-0.

C. Ottmann: A Supramolecular Stabilizer of the 14-3-3ζ/ERα Protein-Protein Interaction with a Synergistic Mode of Action. Angew Chem Int Ed Engl. 2019 Dec 9. doi: 10.1002/anie.201914517.

A. Kuusk, J. F. Neves, K. Bravo Rodriguez, A. Gunnarsson, Y. B. Ruiz-Blanco, M. Ehrmann, H. Chen, I. Landrieu, E. Sanchez-Garcia , H. Boyd, C. Ottmann, R. G. Doveston: Adoption of a Turn Conformation Drives the Binding Affinity of p53 C-Terminal Domain Peptides to 14-3-3σ. ACS Chem. Biol. 2019 Nov doi 10.1021/acschembio.9b00893.

D. Bier, S. Mittal, K. Bravo-Rodriguez, A. Sowislok, X. Guillory, J. Briels, C. Heid, M. Bartel, B. Wettig, L. Brunsveld, E. Sanchez-Garcia , T. Schrader, C. Ottmann: The Molecular Tweezer CLR01 Stabilizes a Disordered Protein-Protein Interface. J Am Chem Soc. 2017 Nov 15;139(45):16256-16263. doi: 10.1021/jacs.7b07939. Epub 2017 Nov 2.

P. de Vink, J. Briels, T. Schrader, L. Milroy, L. Brunsveld, C. Ottmann: Cucurbit[8]uril and 14-3-3 based binary bivalent supramolecular-protein assembly platform and co-crystal structure. Angew Chem Int Ed Engl. 2017 May 16. doi: 10.1002/anie.201701807

D. Bier, M. Bartel, K. Sies, S. Halbach, Y. Higuchi, Y. Haranosono, T. Brummer, N. Kato, C. Ottmann: Small-Molecule Stabilization of the 14-3-3/Gab2 Protein-Protein Interaction (PPI) Interface. ChemMedChem. 2015 Dec 8. doi: 10.1002/cmdc.201500484.

D. Bier, P. Thiel, J. Briels, C. Ottmann: Stabilization of Protein-Protein Interactions in chemical biology and drug discovery. Prog Biophys Mol Biol. 2015, Jun 18. pii: S0079-6107(15)00073-5

M. Skwarczynska, M. Molzan, C. Ottmann: Activation of NF-κB signalling by fusicoccin-induced dimerization. Proc. Natl. Acad Sci. USA 2013, 110, 377-86.

D. Bier, R. Rose, K. Bravo-Rodriguez, M. Bartel, M. Ramirez-Anguita, S. Dutt, F.-G. Klärner, E. Sánchez-Garcia, T. Schrader, C. Ottmann: Molecular tweezers modulate 14-3-3 protein-protein interactions. Nat. Chem. 2013, 5, 234-239.

L. Röglin, P. Thiel, O. Kohlbacher, C. Ottmann: Covalent attachment of pyridoxal-phosphate derivatives to 14-3-3 proteins. Proc. Natl. Acad. Sci. USA, 2012, 109, 1051-1053.

A. Richter, R. Rose, C. Hedberg, H. Waldmann, C. Ottmann: An Optimised Small-Molecule Stabiliser of the 14-3-3-PMA2 Protein-Protein Interaction. Chemistry 2012, 18, 6520-6527.

R. Rose, S. Erdmann, S. Bovens, A. Wolf, M. Rose, S. Hennig, H. Waldmann, C. Ottmann: Identification of small molecule stabilizers of 14-3-3 protein-protein interactions. Angew. Chem. Int. Ed. 2010, 49, 4129-4132.

C. Ottmann, M. Weyand, T. Sassa, T. Inoue, N. Kato, A. Wittinghofer, C. Oecking: A structural rationale for selective stabilization of anti-tumor interactions of 14-3-3 proteins by cotylenin A. J. Mol. Biol. 2009, 386, 913-919.

C. Ottmann, L. Yasmin, M. Weyand, J. L. Veesenmeyer, M. H. Diaz, R. H. Palmer, M. S. Francis, A. R. Hauser, A. Wittinghofer, B. Hallberg: Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis. EMBO J. 2007, 26, 902-913.

C. Ottmann, S. Marco, N. Jaspert, C. Marcon, N. Schauer, M. Weyand, C. Vandermeeren, G. Duby, M. Boutry, A. Wittinghofer, J. L. Rigaud, C. Oecking: Structure of a 14-3-3 coordinated hexamer of the plant plasma mebrane H+-ATPase by combining x-ray crystallography and electron cryomicroscopy. Mol. Cell 2007, 25, 427-440.

More Publications Area B

  • Project B1
    Supramolecular ligands modulate assembly and function of HtrA proteases (Michael Ehrmann)
  • Project B2
    Probing mechanisms of the Cdc48/p97 segregase with designed supramolecular ligands (Hemmo Meyer)
  • Project B3
    Supramolecular specific inhibitors of intestinal proteases against ischemia/reperfusion injury (Herbert de Groot)
  • Project B4
  • Project B5
    Dissection and modulation of (patho)biological Survivin functions by supramolecular ligands (Shirley Knauer)
  • Project B6
    Targeting centromer recruitment of mitotic regulators by supramolecular ligands (Andrea Musacchio)

Chemical Ligands and Methods Publications Area A

  • Project A1
    Protein-recognition by supramolecular ligands from focused combinatorial libraries (Carsten Schmuck)
  • Project A2
    Natural products as starting structures for the development of supramolecular ligands (Markus Kaiser)
  • Project A3
    Designed copolymers and molecular tweezers for protein surface recognition (Thomas Schrader)
  • Project A5
    Protein-specific nanoparticles for cellular uptake (Matthias Epple)
  • Project A6
    DNA-based nanocontainers for predesigned spatial confinement of proteins (Barbara Saccà)
  • Project A7
    Pareto-optimization of protein-surface targeting supramolecular binders with hetero-avidity (Daniel Hoffmann)
  • Project A8
    Exploring protein-recognition by supramolecular binders with MD and QM/MM methods (Elsa Sánchez-García)
  • Project A9
    Raman spectroscopic monitoring of protein recognition by supramolecular ligands (Sebastian Schlücker)