CRC 1093 - Publications Project B6

Area B: Biological Targets

Publications by Hon. Prof. Dr. Andrea Mussachio

Project B6:
Targeting centromere recruitment of mitotic regulators by supramolecular ligands

K. Walstein, A. Petrovic, D. Pan, B. Hagemeier, D. Vogt, I. R. Vetter, A. Musacchio: Assembly principles and stoichiometry of a complete human kinetochore module. Sci Adv 2021 Jun 30;7(27):eabg1037. doi: 10.1126/sciadv.abg1037.

P. Singh, M. E. Pesenti, S. Maffini, S. Carmignani, M. Hedtfeld, A. Petrovic, A. Srinivasamani, T. Bange, A. Musacchio: BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis. Mol Cell. 2021 Jan 7;81(1):67-87.e9. doi: 10.1016/j.molcel.2020.10.040.

V. Piano, A. Alex, P. Stege, S. Maffini, G. A. Stoppiello, P. J. Huis in ‘t Veld, I. Vetter, A. Musacchio: CDC20 assists its catalytic incorporation in the mitotic checkpoint complex. Science. 2021. Jan 1;371(6524):67-71. doi: 10.1126/science.abc1152.

A. Alex, V. Piano, S. Polley, M. Stuiver, S. Voss, G. Ciossani, K. Overlack, B. Voss, S. Wohlgemuth, A. Petrovic, Y. Wu, P. Selenko, A. Musacchio *, S. Maffini S*: Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology. Elife. 2019 e48287. doi: 10.7554/eLife.48287. *co-corresponding authors.

D. Pan, K. Walstein, A. Take, D. Bier, N. Kaiser, A. Musacchio:Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing Nat Commun. 2019 Sep 6;10(1):4046. doi: 10.1038/s41467-019-12019-6.

M. E. Pesenti, D. Prumbaum, P. Auckland, C. M. Smith, A. C. Faesen, A. Petrovic, M. Erent, S. Maffini,1 S. Pentakota, J. R. Weir, Y.-C. Lin, S. Raunser, A. D. McAinsh, A. Musacchio: Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80. Mol Cell. 2018 Sep 20; 71(6): 923–939.e10. doi:10.1016/j.molcel.2018.07.038.

V. A. Volkov, P. J. Huis In 't Veld, M. Dogterom, A. Musacchio: Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces. Elife. 2018 Apr 9;7. pii: e36764. doi: 10.7554/eLife.36764.

A. Petrovic, J. Keller, Y. Liu, K. Overlack, J. John, Y. Dimitrova, S. Jenni, S. van Gerwen, P. Stege, S. Wohlgemuth,P. Rombaut, F. Herzog, S.C. Harrison, I. Vetter, A. Musacchio: Structure of the MIS12 complex and molecular basis of its interaction with CENP-C at human kinetochores. Cell 2016 167:1020-1040.

J.R. Weir, A.C. Faesen, K. Klare, A. Petrovic, F. Basilico, J. Fischböck, S. Pentakota, J. Keller, M.E. Pesenti, D. Pan, D. Vogt, S. Wohlgemuth, F. Herzog, A. Musacchio: Insights from biochemical reconstitution into the architecture of human kinetochores. Nature. 2016 Aug 31;537(7619):249-253. doi: 10.1038/nature19333. Epub ahead of print

A. Friese, A.C. Faesen, P. J. Huis In 't Veld, J. Fischböck, D. Prumbaum, A. Petrovic, S. Raunser, F. Herzog, A. Musacchio: Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin. Nat Commun. 2016 7:11407. doi: 10.1038/ncomms11407.

V. Krenn, A. Musacchio: The Aurora B Kinase in Chromosome Bi-Orientation and Spindle Checkpoint Signaling. Front Oncol. 2015, 5:225. doi: 10.3389/fonc.2015.00225.

A. Musacchio: The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics. Curr Biol. 2015, 25:R1002-18. doi: 10.1016/j.cub.2015.08.051.

A. Altenfeld, S. Wohlgemuth, A. Wehenkel, I.R. Vetter, A. Musacchio: Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex. Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):438-42. doi: 10.1107/S2053230X15004343. Epub 2015 Mar 20.

A. Eiteneuer, J. Seiler, M. Weith, M. Beullens, B. Lesage, V. Krenn, A. Musacchio, M. Bollen, H. Meyer: Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 to kinetochore-bound protein phosphatase-1. EMBO J.. 2014, Nov 18;33(22):2704-20.

V. Krenn, A. Wehenkel, X. Li, S. Santaguida & A. Musacchio: Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction. J. Cell Biol. 2012, 196, 451-467.

A. De Antoni, S. Maffini, S. Knapp, A. Musacchio* & S. Santaguida: A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B. J. Cell Biol. 2012, 199, 269-284. (* co-corresponding author)

S. Santaguida, C. Vernieri, F. Villa, A. Ciliberto & A. Musacchio: Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction. EMBO J. 2011, 30, 1508-1519.

A. Petrovic, S. Pasqualato, P. Dube, V. Krenn, S. Santaguida, D. Cittaro, S. Monzani, L. Massimiliano, J. Keller, A. Tarricone, A. Maiolica, H. Stark & A. Musacchio: The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J. Cell Biol. 2010, 190, 835-852.

S. Santaguida, A. Tighe, A. M. D'Alise, S. S. Taylor & A. Musacchio: Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J. Cell Biol. 2010, 190, 73-87.

G. M. Alushin, V. H. Ramey, S. Pasqualato, D. A. Ball, N. Grigorieff, A. Musacchio & E. Nogales: The Ndc80 kinetochore complex forms oligomeric arrays along microtubules. Nature 2010, 467, 805-10. 

F. Villa et al.: Crystal structure of the catalytic domain of Haspin, an atypical kinase implicated in chromatin organization. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 20204-20209.

S. Santaguida & A. Musacchio: The life and miracles of kinetochores. EMBO J. 2009, 28, 2511–2531.

C. Ciferri, S. Pasqualato, E. Screpanti & A. Musacchio: Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex. Cell 2008, 133, 427-439.

More Publications Area B

  • Project B1
    Supramolecular ligands modulate assembly and function of HtrA proteases (Michael Ehrmann)
  • Project B2
    Probing mechanisms of the Cdc48/p97 segregase with designed supramolecular ligands (Hemmo Meyer)
  • Project B3
    Supramolecular specific inhibitors of intestinal proteases against ischemia/reperfusion injury (Herbert de Groot)
  • Project B4
    Modulation of 14-3-3 protein-protein interactions by supramolecular chemistry (Christian Ottmann)
  • Project B5
    Dissection and modulation of (patho)biological Survivin functions by supramolecular ligands (Shirley Knauer)
  • Project B6

Chemical Ligands and Methods Publications Area A

  • Project A1
    Protein-recognition by supramolecular ligands from focused combinatorial libraries (Carsten Schmuck)
  • Project A2
    Natural products as starting structures for the development of supramolecular ligands (Markus Kaiser)
  • Project A3
    Designed copolymers and molecular tweezers for protein surface recognition (Thomas Schrader)
  • Project A5
    Protein-specific nanoparticles for cellular uptake (Matthias Epple)
  • Project A6
    DNA-based nanocontainers for predesigned spatial confinement of proteins (Barbara Saccà)
  • Project A7
    Pareto-optimization of protein-surface targeting supramolecular binders with hetero-avidity (Daniel Hoffmann)
  • Project A8
    Exploring protein-recognition by supramolecular binders with MD and QM/MM methods (Elsa Sánchez-García)
  • Project A9
    Raman spectroscopic monitoring of protein recognition by supramolecular ligands (Sebastian Schlücker)