CRC 1093 - Publications Project A5

Area A: Chemical Ligands and Method

Publications by Prof. Dr. Matthias Epple

Project A5:
Protein-specific nanoparticles for cellular uptake

K. Klein, K. Loza, M. Heggen, M. Epple: An Efficient Method for Covalent Surface Functionalization of Ultrasmall Metallic Nanoparticles by Surface Azidation Followed by Copper-Catalyzed Azide-Alkyne Cycloaddition (Click Chemistry). Chem Nano Mat 2021 Oct 22; doi: 10.1002/cnma.202100359

O. Wetzel, S. Hosseini, K. Loza, Kateryna, M. Heggen, O. Prymak, P. Bayer, C. Beuck, T. Schaller, F. Niemeyer, C. Weidenthaler, M. Epple: Metal-ligand interface and internal structure of ultrasmall silver nanoparticles (2 nm). J Phys Chem B 2021; 125. doi: 10.1021/acs.jpcb.1c02512

V. Sokolova, M. Epple: Biological and medical applications of calcium phosphate nanoparticles. Chem Eur J 2021 May 12; 27(27):7471-7488. doi: 10.1002/chem.202005257

T. Ruks, K. Loza, M. Heggen, O. Prymak, A. L. Sehnem, C. L. P. Oliveira, P. Bayer, C. Beuck, M. Epple: Peptide-conjugated ultrasmall gold nanoparticles (2 nm) for selective protein targeting. ACS Applied Bio Materials 2021; 4 (1): 945-965. doi: 10.1021/acsabm.0c01424

T. Ruks, K. Loza, M. Heggen, C. Ottmann, P. Bayer, C. Beuck, M. Epple: Targeting the surface of the protein 14-3-3 by ultrasmall gold nanoparticles (1.5 nm), carrying the specific peptide CRaf. Chem Bio Chem 2021 Apr 16;22(8):1456-1463. doi: 10.1002/cbic.202000761.

S. B. van der Meer, I. Hadrovic, A. Meiners, K. Loza, M. Heggen, S. Knauer, P. Bayer, T. Schrader, C. Beuck,M. Epple: New Tools to Probe the Protein Surface: Ultrasmall Gold Nanoparticles Carry Amino Acid Binders. J Phys Chem B 2021 Jan 14;125(1):115-127. doi: 10.1021/acs.jpcb.0c09846

S. B. van der Meer, T. Seiler, C. Buchmann, G. Partalidou, S. Boden, K. Loza, M. Heggen, J. Linders, O. Prymak, L. Hartmann, C. L. P. Oliveira, M. Epple: Controlling the surface functionalization of ultrasmall gold nanoparticles by sequence-defined macromolecules. Chem Eur J 2021 Jan 18; 27(4):1451-1464. doi: 10.1002/chem.202003804

K. Wey, M. Epple: Ultrasmall gold and silver/gold nanoparticles (2 nm) as autofluorescent labels for poly(D,L-lactide-co-glycolide) nanoparticles (140 nm), J. Mater. Sci. Mater. Med. 2020 Nov 28;31(12):117. doi 10.1007/s10856-020-06449-8.

L. Rojas-Sánchez, K. Loza, M. Epple: Synthesis and intracellular tracing of surface-functionalized calcium phosphate nanoparticles by super-resolution microscopy (STORM), Materialia 2020; 12:100773. doi: 10.1016/j.mtla.2020.100773.

V. Sokolova, G. Mekky, S. B. van der Meer, M. C. Seeds, A. J. Atala, M. Epple: Transport of ultrasmall gold nanoparticles (2 nm) across the blood-brain barrier in a six-cell brain spheroid model. Sci Rep 2020 Oct 22;10(1):18033. doi: 10.1038/s41598-020-75125-2.

S. Kollenda, M. Kopp, J. Wens, J. Koch, N. Schulze, C. Papadopoulos, R. Pöhler, H. Meyer, M. Epple: A pH-sensitive fluorescent protein sensor to follow the pathway of calcium phosphate nanoparticles into cells. Acta Biomater. 2020 Jul 15;111:406-417. doi: 10.1016/j.actbio.2020.05.014.

V. Sokolova, G. Nzou, S. B. van der Meer, T. Ruks, M. Heggen, K. Loza, N. Hagemann, F. Murke, B. Giebel, D. M. Hermann, A. J. Atala, M. Epple: Ultrasmall gold nanoparticles (2 nm) can penetrate and enter cell nuclei in an in vitro 3D brain spheroid model. Acta Biomater. 2020 Jul 15;111:349-362. doi: 10.1016/j.actbio.2020.04.023.

S. B. van der Meer, K. Loza, K. Wey, M. Heggen, C. Beuck, P. Bayer, M. Epple: Click chemistry on the surface of ultrasmall gold nanoparticles (2 nm) for covalent ligand attachment followed by NMR spectroscopy”, Langmuir 2019, 35, 6288-6296.

L. Rojas-Sanchez, V. Sokolova, S. Riebe, J. Voskuhl, M. Epple: Covalent Surface Functionalization of Calcium Phosphate Nanoparticles with Fluorescent Dyes by Copper-Catalysed and by Strain-Promoted Azide-Alkyne Click Chemistry. ChemNanoMat 2019, 5, 436 –446, DOI: 10.1002/cnma.201800509

T. Ruks, C. Beuck, T. Schaller, F. C. Niemeyer, M. Zähres, K. Loza, M. Heggen, U. Hagemann, C. Mayer, P. Bayer, M. Epple: Solution NMR spectroscopy with isotope-labelled cysteine (13C, 15N) reveals the surface structure of L-cysteine-coated ultrasmall gold nanoparticles (1.8 nm). Langmuir 2019, 35 (3), 767-778 DOI: 10.1021/acs.langmuir.8b03840

V. Sokolova, L. Rojas-Sánchez, N. Białas, N. Schulze, M. Epple: Calcium phosphate nanoparticle-mediated transfection in 2D and 3D mono- and co-culture cell models. Acta Biomaterialia 2019, 84 391–401

M. Kopp , S. Kollenda, M. Epple: Nanoparticle-protein interactions: Therapeutic approaches and supramolecular chemistry. Acc. Chem. Res. 2017, 50 (6), pp 1383–1390, doi: 10.1021/acs.accounts.7b00051

M. Kopp, O. Rotan, C. Papadopoulos, N. Schulze, H. Meyer, M. Epple: Delivery of the autofluorescent protein R-phycoerythrin by calcium phosphate nanoparticles into four different eukaryotic cell lines (HeLa, HEK293T, MG-63, MC3T3): Highly efficient, but leading to endolysosomal proteolysis in HeLa and MC3T3 cells. PLOS ONE 2017, June 6, https://doi.org/10.1371/journal.pone.0178260

S. Chernousova , M. Epple: Live-cell imaging to compare the transfection and gene silencing efficiency of calcium phosphate nanoparticles and a liposomal transfection agent. Gene Therapy. 2017, 24, 282–289; doi:10.1038/gt.2017.13

O. Rotan, K. N. Severin, S. Pöpsel, A. Peetsch, M. Merdanovic, M. Ehrmann, M. Epple: Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles. Beilstein J. Nanotechn. 2017, 8, 381–393.

B. Schuetze, C. Mayer, K. Loza, M. Gocyla, M. Heggen, M. Epple: The conjugation of thiol-terminated molecules to ultrasmall 2 nm-gold nanoparticles leads to remarkably complex 1H-NMR spectra. Journal of Materials Chemistry B. 2016, 4, 2179-2189.

K. Haedicke, D. Kozlova, S. Gräfe, U. Teichgräber, M. Epple*, I. Hilger*: Multi-functional calcium phosphate nanoparticles for combining near-infrared fluorescence optical imaging and photodynamic therapy. Acta Biomaterialia. 2015, 14, 197-207.*corresponding author

G. Dördelmann, D. Kozlova, S. Karczewski, R. Lizio, S. Knauer, M. Epple: Calcium phosphate increases the encapsulation efficiency of hydrophilic drugs (proteins, nucleic acids) into poly (D,L-lactide-co-glycolide acid) nanoparticles for intracellular delivery. Journal of Materials Chemistry B. 2014, 2, 7250.

O. Rotan, V. Sokolova, P. Gilles, W. Hu, S. Dutt, T. Schrader and M. Epple: Transport of supramolecular drugs across the cell membrane by calcium phosphate nanoparticles. Mat. Sci. Engin. Techn. 2013, 44, 2.

A. Kovtun, S. Neumann, M. Neumeier, H. Urch, R. Heumann, M.M. Gepp, K. Wallat, M. Koeller, H. Zimmermann, M. Epple: Nanoparticle-mediated gene transfer from electrophoreti-cally coated metal surfaces. J. Phys. Chem. B 2013, 117, 1550-1555.

V. Sokolova, O. Rotan, J. Klesing, P. Nalbant, J. Buer, T. Knuschke, A.M. Westendorf, M. Epple: Calcium phosphate nanoparticles as versatile carrier for small and large molecules across cell membranes. J. Nanoparticle Res. 2012, 14, 910.

D. Kozlova, S. Chernousova, T. Knuschke, J. Buer, A.M. Westendorf, M. Epple: Cell targeting by antibody-functionalized calcium phosphate nanoparticles. J. Mater. Chem. 2012, 22, 396-404.

M. Neumeier, L. Hails, S. Mann, M. Epple: Synthesis of fluorescent core-shell hydroxyap-atite nanoparticles. J. Mater. Chem. 2011, 21, 1250-1254.

S. Kittler, C. Greulich, J. Diendorf, M. Köller, M. Epple: Toxicity of silver nanoparticles increases during storage because of slow dissolution under release of silver ions. Chem. Mater., 2010, 22, 4548-4554.

D. Mahl, C. Greulich, W. Meyer-Zaika, M. Köller, M. Epple: Gold nanoparticles: dispersabil-ity in biological media and cell-biological effect. J. Mater. Chem. 2010, 20, 6176-6181.

V. Sokolova, T. Knuschke, A. Kovtun, J. Buer, M. Epple, A.M. Westendorf: The use of cal-cium phosphate nanoparticles encapsulating toll-like receptor ligands and the antigen hemagglutinin to induce dendritic cell maturation and T cell activation. Biomaterials 2010, 31, 5627-5633.

S. Neumann, A. Kovtun, I.D. Dietzel, M. Epple, R. Heumann: The use of size-defined DNA-functionalized calcium phosphate nanoparticles to minimise intracellular calcium disturbance during transfection. Biomaterials 2009, 30, 6794-6802.

More Publications Area A

  • Project A1
    Protein-recognition by supramolecular ligands from focused combinatorial libraries (Carsten Schmuck)
  • Project A2
    Natural products as starting structures for the development of supramolecular ligands (Markus Kaiser)
  • Project A3
    Designed copolymers and molecular tweezers for protein surface recognition (Thomas Schrader)
  • Project A5
  • Project A6
    DNA-based nanocontainers for predesigned spatial confinement of proteins (Barbara Saccà)
  • Project A7
    Pareto-optimization of protein-surface targeting supramolecular binders with hetero-avidity (Daniel Hoffmann)
  • Project A8
    Exploring protein-recognition by supramolecular binders with MD and QM/MM methods (Elsa Sánchez-Garcia)
  • Project A9
    Raman spectroscopic monitoring of protein recognition by supramolecular ligands (Sebastian Schlücker)

Biological Targets Publications Area B

  • Project B1
    Supramolecular ligands modulate assembly and function of HtrA proteases (Michael Ehrmann)
  • Project B2
    Probing mechanisms of the Cdc48/p97 segregase with designed supramolecular ligands (Hemmo Meyer)
  • Project B3
    Supramolecular specific inhibitors of intestinal proteases against ischemia/reperfusion injury (Herbert de Groot)
  • Project B4
    Modulation of 14-3-3 protein-protein interactions by supramolecular chemistry (Christian Ottmann)
  • Project B5
    Dissection and modulation of (patho)biological Survivin functions by supramolecular ligands (Shirley Knauer)
  • Project B6
    Targeting centromer recruitment of mitotic regulators by supramolecular ligands (Andrea Musacchio)