CRC 1093 - Publications Project B1

Area B: Biological Targets

Publications by Prof. Dr. Michael Ehrmann

Project B1:
Supramolecular ligands modulate assembly and function of HtrA proteases

M. Merdanovic, S. G. Burston, A. L. Schmitz, S. Köcher, S. Knapp, T. Clausen, M. Kaiser, R. Huber, M. Ehrmann: Activation by substoichiometric inhibition. Proc Natl Acad Sci U S A. 2020 Jan 21;117(3):1414-1418. doi: 10.1073/pnas.1918721117. Epub 2020 Jan 6.

K. Bravo-Rodriguez, B. Hagemeier, L. Drescher, M. Lorenz, J. Rey, M. Meltzer, F. Kaschani, M. Kaiser, M. Ehrmann: Utilities for Mass Spectrometry Analysis of Proteins: Fast post processing of mass spectrometry data. Rapid Commun Mass Spectrom. 2018 Jul 31. doi: 10.1002/rcm.8243.

Schillinger J, Severin K, Kaschani F, M. Kaiser, M. Ehrmann: HTRA1-Dependent Cell Cycle Proteomics. J. Proteome Res. 2018 Aug 3;17(8):2679-2694. doi: 10.1021/acs.jproteome.8b00129. Epub 2018 Jul 13.

J. Bongard, M. Lorenz, I. R. Vetter , P. Stege, A. T. Porfetye, A. L. Schmitz, F. Kaschani, A. Wolf, U. Koch, P. Nussbaumer, B. Klebl, M. Kaiser, M. Ehrmann: Identification of Noncatalytic Lysine Residues from Allosteric Circuits via Covalent Probes. ACS Chem. Biol. 2018, Article ASAP, DOI: 10.1021/acschembio.8b00101 Publication Date (Web): April 16, 2018

S. Poepsel, A. Sprengel, B. Sacca, F. Kaschani , M. Kaiser, C. Gatsogiannis, S. Raunser, T. Clausen and M. Ehrmann: Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature Chemical Biology. 2015, doi:10.1038/nchembio.1931

M. Ehrmann, F. Kaschani , M. Kaiser: Chemical proteomics versus Leishmaniasis. Chem. Biol. 2015, 22:309-310

N. J. Thompson, M. Merdanovic, M. Ehrmann, E. van Duijn, A. J. R. Heck: Substrate Occupancy at the Onset of Oligomeric Transitions of DegP. Structure 2015, 22: 281–290

M. Mastny, A. Heuck, R. Kurzbauer, A. Heiduk, P. Boisguerin, R. Volkmer, M. Ehrmann, C. D. A. Rodriguez, D. Z. Rudner, T. Clausen: CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis. Cell 2013, 155:647–658

M. Kaiser, R. Huber, M. Ehrmann: Proteolysis. Encyclopedia of Genetics 2013, S. Maloy and K. Hughes (eds.), Academic Press, p 501-503

S.C. Stolze, M. Meltzer, M. Ehrmann, M. Kaiser: Ahp cyclodepsipeptides: the impact of the Ahp residue on the "canonical inhibition" of S1 serine proteases. Chembiochem. 2013, Jul 22;14(11):1301-8.

S. Sinha, D. H. J. Lopes, Z. Du, E. S. Pang, A. Shanmugam, A. Lomakin, P. Talbiersky, A. Tennstaedt, K. McDaniel, R. Bakshi, P.-Y. Kuo, M. Ehrmann, G. B. Benedek, J. A. Loo, F.-G. Klärner, T. Schrader, C. Wang, G. Bitan: Lysine-specific molecular tweezers are broad-spectrum inhibitors of assembly and toxicity of amy   loid proteins. J. Am. Chem. Soc. 2011, 133, 16958–16969.

L. Trübestein, A. Tennstaedt, T. Mönig, T.  Krojer, F. Canellas, M. Kaiser, T. Clausen, M. Ehrmann: Substrate induced remodeling of the active site regulates human HTRA1 activity. Nat. Struct. Mol. Biol. 2011, 18, 386-388.

T. Clausen, M. Kaiser, R. Huber, M. Ehrmann: HtrA proteases: Regulated proteolysis in protein quality control. Nat. Rev. Mol. Cell Biol. 2011, 12, 152-162.

M. Merdanovic, N. Mamant, M. Meltzer, S. Poepsel, A. Auckenthaler, R. Melgaard, P. Hauske, L. Nagel-Steger, A. R. Clarke, M. Kaiser, R. Huber, M. Ehrmann: Determinants of structural and functional plasticity of a widely conserved protease chaperone complex. Nat. Struct. Mol. Biol. 2010, 17, 837-843.

T. Krojer, J. Sawa, E. Schäfer, H. R. Saibil, M. Ehrmann, T. Clausen: Structural basis for the regulated protease and chaperone function of DegP. Nature 2008, 453, 885-890.

M. Meltzer, S. Hasenbein, P. Hauske, N. Kucz, M. Merdanovic, S. Grau, A. Beil, D. Jones, T. Krojer, T. Clausen, M. Ehrmann, M. Kaiser: Allosteric activation of HtrA protease DegP by stress signals    in bacterial protein quality control. Angew. Chem. Int. Ed. 2008, 47, 1332-1334.

C. Wilken, K. Kitzing, R. Kurzbauer, M. Ehrmann, T. Clausen: Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease. Cell  2004, 117, 483-494.

C. Spiess, A. Beil, M. Ehrmann: A temperature-dependent switch from chaperone to protease in a widely conserved heat shock protein. Cell 1999, 97, 339-347.

More Publications Area B

  • Project B1
  • Project B2
    Probing mechanisms of the Cdc48/p97 segregase with designed supramolecular ligands (Hemmo Meyer)
  • Project B3
    Supramolecular specific inhibitors of intestinal proteases against ischemia/reperfusion injury (Herbert de Groot)
  • Project B4
    Modulation of 14-3-3 protein-protein interactions by supramolecular chemistry (Christian Ottmann)
  • Project B5
    Dissection and modulation of (patho)biological Survivin functions by supramolecular ligands (Shirley Knauer)
  • Project B6
    Targeting centromer recruitment of mitotic regulators by supramolecular ligands (Andrea Musacchio)

Chemical Ligands and Methods Publications Area A

  • Project A1
    Protein-recognition by supramolecular ligands from focused combinatorial libraries (Carsten Schmuck)
  • Project A2
    Natural products as starting structures for the development of supramolecular ligands (Markus Kaiser)
  • Project A3
    Designed copolymers and molecular tweezers for protein surface recognition (Thomas Schrader)
  • Project A5
    Protein-specific nanoparticles for cellular uptake (Matthias Epple)
  • Project A6
    DNA-based nanocontainers for predesigned spatial confinement of proteins (Barbara Saccà)
  • Project A7
    Pareto-optimization of protein-surface targeting supramolecular binders with hetero-avidity (Daniel Hoffmann)
  • Project A8
    Exploring protein-recognition by supramolecular binders with MD and QM/MM methods (Elsa Sánchez-García)
  • Project A9
    Raman spectroscopic monitoring of protein recognition by supramolecular ligands (Sebastian Schlücker)