Download our operating protocol.

And take a look to the whole mount staining iDISCO-Protocol (vers. 2016)

Technical Details:

LaVision BioTec Ultramicroscope fluorescence light sheet imaging for 3D image reconstruction in large samples . Cleared wholemount specimens are illuminated using a thin light sheet and imaged on a high resolution sCMOS camera orthogonal to illumination. Selective illumination ensures decreased photobleaching. System is equipped with dual lightsheet illumination.

Microscope:
The system is build by Milteniy Biotec. It offers three different objectives (1.1x, 4x and 12x). Additionally, you can choose a zoom of 0.6x, 1x or 2.5x for each objective. The minimal light sheet beam wait diameter (i.e., highest possible z resolution) of the system is in the order of 4 µm. Light sheet illumination is possible from both slides, and from three different angles on each side (i.e., 6 beams total) to minimize shadowing artifacts. The light sheet beam waist can be swept laterally to image every position within the sample with (near-)optimal z resolution. 

Light sheet excitation lines:
White Light laser (200mW) 480-2400nm

Camera:
Andor Neo sCMOS camera: scientific low noise sCMOS camera (6.5µmx6.5µm pixel size).

Detection Filters: