Light MicroscopyLightsheet Ultra Blaze

Download our operating protocol.

And take a look to the whole mount staining iDISCO-Protocol

 

Technical Details:

LaVision BioTec Ultramicroscope fluorescence light sheet imaging for 3D image reconstruction in large samples . Cleared wholemount specimens are illuminated using a thin light sheet and imaged on a high resolution sCMOS camera orthogonal to illumination. Selective illumination ensures decreased photobleaching. System is equipped with dual lightsheet illumination.

 

Microscope:
Is build by Milteniy Biotec with three available objectives (1.1x, 4x and 12x) additionally you can choose a Zoom of 0.6x, 1x or 2.5x for each objective.

 

Light sheet excitation lines:
White Light laser (200mW) 480-2400nm

 

 

Camera:
Andor Neo sCMOS camera: scientific low noise sCMOS camera (6.5µmx6.5µm pixel size).

 

Detection Filters:

ecxitation filter emission filter example color
 
470/30 525/50 GFP
520/40 585/40 RFP, Cy3
560/40 620/60 Rhodamin
595/20 650/50 AlexaFluor594
630/30 680/30 Cy5
690/20 740/40 Alexa Fluor 700
740/40 824/55 Alexa Fluor 790

 

 

Clearing and Sample Preparation: