Olympus BX51
Light MicroscopyOlympus BX51
Technical Details:
Microscope Frame: Olympus BX51 manual upright epifluorescence microscope, with 100W mercury Lamp.
Objectives:
|
Type |
Mag. |
Contrast |
Numerical Aperture |
Working Distance (mm) |
Immersion |
Pixel Size (µm) |
|---|---|---|---|---|---|---|
|
PlanApo N |
1.25 |
Bright field |
0.04 |
5.1 |
air |
8,38 |
|
UPlanFL N |
4 |
Bright field |
0.13 |
17 |
air |
2,11 |
|
UPlanFL N |
10 |
Bright field |
0.3 |
10 |
air |
0,836 |
|
UPlanSApo |
20 |
Bright field |
0.75 |
0.6 |
air |
0,516 |
|
UPlanApoN |
40 |
Bright field |
0.75 |
0.51 |
air |
0,258 |
|
UplanSApo |
60 |
Bright field |
1.35 |
0.15 |
oil |
0,172 |
Filters:
|
Filter Set |
Excitation |
Dichroic |
Emission |
Fluorophores |
|---|---|---|---|---|
|
DAPI |
360-370 |
400 |
460/50 |
DAPI |
|
NB |
470-490 |
515 |
520 LP |
FITC |
|
FITC |
470/40 |
495 |
525/50 |
eGFP, FITC, Alexa 488 |
|
TxRED |
560/55 |
595 |
645/75 |
Texas Red,TRITC, Cy3, Alexa 546, DsRED |
|
Cy5* |
628/40 |
660 |
692/40 |
Cy5* |
* Please be aware, that the excitation of this Fluorochrome is very week. Make sure you only stain highly expressed structures in your sample.
Camera: Olympus DP70 1.5 Megapixel colour ccd camera.
Software: Cell sens Dimension