IMCES Instruments for Sample Preparation

Medizinisches Forschungszentrum (MFZ), 1st floor, room 1.037 Lab Microwave - Ted Pella BioWave Pro+

Lab microwave with magnetic stirrer and vaccum system for
- quick embedding of electron microscopic samples
- treatment of tissue before paraffin- or immune fluorescence embedding
- decalcification (e.g., of bones)

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Medizinisches Forschungszentrum (MFZ), 1st floor, room 1.031 (Cryo-)Ultramicrotome - Leica EM UC7 with EM FC7

(Cryo-)Ultramicrotome with a video system and a big 40" screen for live workflow observations. The system can be used to generate semi- and ultrathin section either of resin-embedded specimens or of frozen cryo-samples.

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Medizinisches Forschungszentrum (MFZ), 1st floor, room 1.039 Automated Grid Stainer - Leica EM AC20

Automated post-staining system (uranyl acetate + lead citrate) for ultrathin sample sections on standard TEM grids.

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Medizinisches Forschungszentrum (MFZ), 1st floor, room 1.035 Glow Discharger - Pelco Easy Glow

The system is mainly used for hydrophilization and cleaning of TEM grids potentially coated with carbon support films to ensure a better spreading of aqueous solutions.

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Medizinisches Forschungszentrum (MFZ), 1st floor, room 1.035 Critical Point Dryer - Leica EM CPD 300

With this device scanning electron microscopic samples are critical-point-dried which is necessary before sputtering

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Medizinisches Forschungszentrum (MFZ), 1st floor, room 1.037 Sputter Coater - Leica ACE 600

With this device the surfaces of specimens are sputter coated with metals (platinum, palladium, gold or chrome) for better visualisation.

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Medizinisches Forschungszentrum (MFZ), 1st floor, room 1.039 Sputter Coater - Quorum Q150T ES

For some samples it is reasonable to perform carbon sputter coating before investigation on the scanning electron microscope. This device exclusively is used for that purpose.

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Vibratome Leica VT1000S

Vibratome to produce high quality thin sections of fresh or fixed tissue.

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