ZMB Member Bettina Siebers

ZMB Member
Bettina Siebers

Next ZMB-Member
Bettina Siebers portrait

Prof. Dr. Bettina Siebers

Faculty of Chemistry
University of Duisburg-Essen
Universitätsstr. 2
45141 Essen

Research Overview


Since their discovery as a third domain of life by Carl Woese and beside Bacteria and Eukarya Archaea have not only served to study adaptation processes to extreme growth conditions. Being an important third and comparative lineage, they were also instrumental in studying the evolution and characteristics of central cellular functions.

Central carbohydrate metabolism (CCM) in hyperthermophilic Archaea

As an increasing amount of genome sequence information becomes available, one of the major challenges of the post-genome era is to elucidate the function of numerous hypothetical genes which were found in all studied genomes (20-40% of genes).

Biochemistry and Physiology

Many of the proteins of the CCM of T. tenax (about 22; partly in collaboration with R. Hensel) and other hyperthermophiles, either identified by classical reversed genetics or new genomics-based methods, were expressed in Escherichia coli, purified and the predicted activity was confirmed by enzymatic studies.

Transcription and transcription regulation: The archaeal model system

Since there are only three homologs to eukaryal general transcription factors (GTFs) present in Archaea, and because of similar promoter organisation, archaeal transcription is generally accepted to provide a simpler model of the eukaryal transcription. In a project in the Research Training Group ("Transcription, Chromatin Structure and DNA Repair in Development and Differentiation", University of Duisburg-Essen) we work on the regulation of basal transcription, addressing the role of multiple transcription factors (TFB), and the identification of transcriptional regulators in T. tenax.

Systems Biology: "SulfoSYS"

Silicon Cell Model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation.

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Selected Publications

    Journal articles

  • Kuschmierz, Laura; Shen, Lu; Bräsen, Christopher; Snoep, Jacky; Siebers, Bettina
    Workflows for optimization of enzyme cascades and whole cell catalysis based on enzyme kinetic characterization and pathway modelling
    In: Current Opinion in Biotechnology Vol. 74 (2022) pp. 55 - 60
  • Lewis, April M; Recalde, Alejandra; Bräsen, Christopher; Counts, James A; Nussbaum, Phillip; Bost, Jan; Schocke, Larissa; Shen, Lu; Willard, Daniel J; Quax, Tessa E. F; Peeters, Eveline; Siebers, Bettina; Albers, Sonja-Verena; Kelly, Robert M
    The biology of thermoacidophilic archaea from the order Sulfolobales
    In: FEMS Microbiology Reviews Vol. 45 (2021) Nr. 4, fuaa063
  • Shen, Lu; Kohlhaas, Martha; Enoki, Junichi; Meier, Roland; Schönenberger, Bernhard; Wohlgemuth, Roland; Kourist, Robert; Niemeyer, Felix; van Niekerk, David; Bräsen, Christopher; Niemeyer, Jochen; Snoep, Jacky; Siebers, Bettina
    A combined experimental and modelling approach for the Weimberg pathway optimisation
    In: Nature Communications Vol. 11 (2020) Nr. 1, pp. 1098
  • Wang, Kun; Sybers, David; Maklad, Hassan Ramadan; Lemmens, Liesbeth; Lewyllie, Charlotte; Zhou, Xiaoxiao; Schult, Frank; Bräsen, Christopher; Siebers, Bettina; Valegård, Karin; Lindås, Ann-Christin; Peeters, Eveline
    A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldarius
    In: Nature Communications Vol. 10 (2019) Nr. 1, pp. 1542
  • Schocke, Larissa; Bräsen, Christopher; Siebers, Bettina
    Thermoacidophilic Sulfolobus species as source for extremozymes and as novel archaeal platform organisms
    In: Current Opinion in Biotechnology Vol. 59 (2019) pp. 71 - 77
  • Wohlgemuth, Roland; Littlechild, Jennifer; Monti, Daniela; Schnorr, Kirk; van Rossum, Teunke; Siebers, Bettina; Menzel, Peter; Kublanov, Ilya V.; Rike, Anne Gunn; Skretas, Georgios; Szabo, Zalan; Peng, Xu; Young, Mark J.
    Discovering novel hydrolases from hot environments
    In: Biotechnology Advances Vol. 36 (2018) Nr. 8, pp. 2077 - 2100
  • Schult, Frank; Le, Thuong N.; Albersmeier, Andreas; Rauch, Bernadette; Blumenkamp, Patrick; Van Der Does, Chris; Goesmann, Alexander; Kalinowski, Jörn; Albers, Sonja-Verena; Siebers, Bettina
    Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response
    In: Nucleic Acids Research Vol. 46 (2018) Nr. 14, pp. 7179 - 7192
  • Knüppel, Robert; Christensen, Regitse H; Gray, Fiona C; Esser, Dominik; Strauß, Daniela; Medenbach, Jan; Siebers, Bettina; MacNeill, Stuart A; LaRonde, Nicole; Ferreira-Cerca, Sébastien
    Insights into the evolutionary conserved regulation of Rio ATPase activity.
    In: Nucleic Acids Research Vol. 46 (2018) Nr. 3, pp. 1441 - 1456
  • Zweerink, Susanne; Kallnik, Verena; Ninck, Sabrina; Nickel, Sabrina; Verheyen, Julia; Blum, Marcel; Wagner, Alexander; Feldmann, Ingo; Sickmann, Albert; Albers, Sonja-Verena; Bräsen, Christopher; Kaschani, Farnusch; Siebers, Bettina; Kaiser, Markus
    Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea
    In: Nature Communications Vol. 8 (2017) pp. 15352
  • Esser, Dominik; Hoffmann, Lena; Pham, Trong Khoa; Bräsen, Christopher; Qiu, Wen; Wright, Phillip C.; Albers, Sonja-Verena; Siebers, Bettina
    Protein phosphorylation and its role in archaeal signal transduction
    In: FEMS Microbiology Reviews Vol. 40 (2016) Nr. 5, pp. 625 - 647
  • Nelson-Sathi, Shijulal; Sousa, Filipa L.; Roettger, Mayo; Lozada-Chávez, Nabor; Thiergart, Thorsten; Janssen, Arnold; Bryant, David; Landan, Giddy; Schönheit, Peter; Siebers, Bettina; McInerney, James O.; Martin, William F.
    Origins of major archaeal clades correspond to gene acquisitions from bacteria
    In: Nature Vol. 517 (2015) Nr. 7532, pp. 77 - 80