ZMB Member Bettina Siebers
ZMB Member
Bettina Siebers
Next ZMB-Member
Prof. Dr. Bettina Siebers
Faculty of Chemistry
University of Duisburg-Essen
Universitätsstr. 2
45141 Essen
- +49 201 183 7061
- Website
- Selected Publications
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- ZMB Research Program
Immunology, Infectious Diseases and Transplantation
Research Overview
Archaea
Since their discovery as a third domain of life by Carl Woese and beside Bacteria and Eukarya Archaea have not only served to study adaptation processes to extreme growth conditions. Being an important third and comparative lineage, they were also instrumental in studying the evolution and characteristics of central cellular functions.
Central carbohydrate metabolism (CCM) in hyperthermophilic Archaea
As an increasing amount of genome sequence information becomes available, one of the major challenges of the post-genome era is to elucidate the function of numerous hypothetical genes which were found in all studied genomes (20-40% of genes).
Biochemistry and Physiology
Many of the proteins of the CCM of T. tenax (about 22; partly in collaboration with R. Hensel) and other hyperthermophiles, either identified by classical reversed genetics or new genomics-based methods, were expressed in Escherichia coli, purified and the predicted activity was confirmed by enzymatic studies.
Transcription and transcription regulation: The archaeal model system
Since there are only three homologs to eukaryal general transcription factors (GTFs) present in Archaea, and because of similar promoter organisation, archaeal transcription is generally accepted to provide a simpler model of the eukaryal transcription. In a project in the Research Training Group ("Transcription, Chromatin Structure and DNA Repair in Development and Differentiation", University of Duisburg-Essen) we work on the regulation of basal transcription, addressing the role of multiple transcription factors (TFB), and the identification of transcriptional regulators in T. tenax.
Systems Biology: "SulfoSYS"
Silicon Cell Model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation.
Selected Publications
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A combined experimental and modelling approach for the Weimberg pathway optimisationIn: Nature Communications Vol. 11 (2020) Nr. 1, pp. 1098
ISSN: 2041-1723Online Full Text: dx.doi.org/ (Open Access) -
A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldariusIn: Nature Communications Vol. 10 (2019) Nr. 1, pp. 1542
ISSN: 2041-1723Online Full Text: dx.doi.org/ (Open Access) -
Thermoacidophilic Sulfolobus species as source for extremozymes and as novel archaeal platform organismsIn: Current Opinion in Biotechnology Vol. 59 (2019) pp. 71 - 77
ISSN: 1879-0429; 0958-1669Online Full Text: dx.doi.org/ -
Discovering novel hydrolases from hot environmentsIn: Biotechnology Advances Vol. 36 (2018) Nr. 8, pp. 2077 - 2100
ISSN: 0734-9750; 1873-1899Online Full Text: dx.doi.org/ (Open Access) -
Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV responseIn: Nucleic Acids Research Vol. 46 (2018) Nr. 14, pp. 7179 - 7192
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ Online Full Text (Open Access) -
Insights into the evolutionary conserved regulation of Rio ATPase activity.In: Nucleic Acids Research Vol. 46 (2018) Nr. 3, pp. 1441 - 1456
ISSN: 1362-4962; 0301-5610; 0305-1048Online Full Text: dx.doi.org/ (Open Access) -
Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic ArchaeaIn: Nature Communications Vol. 8 (2017) pp. 15352
ISSN: 2041-1723Online Full Text: dx.doi.org/ (Open Access) -
Protein phosphorylation and its role in archaeal signal transductionIn: FEMS Microbiology Reviews Vol. 40 (2016) Nr. 5, pp. 625 - 647
ISSN: 1574-6976; 0168-6445Online Full Text: dx.doi.org/ (Open Access) -
Origins of major archaeal clades correspond to gene acquisitions from bacteriaIn: Nature Vol. 517 (2015) Nr. 7532, pp. 77 - 80
ISSN: 0028-0836; 1476-4687Online Full Text: dx.doi.org/ Online Full Text (Open Access)