Axio Scan Z.1
Light MicroscopyZeiss Axio Scan Z.1
Technical Details:
Microscope frame: Zeiss Axio Scan Z.1 motorized epifluorescence and transmitted light microscope.
Light Source: Illumination with Colibri 7 high performance LED at 385nm, 430nm, 475nm, 555nm, 590nm, 630nm and 735nm.
Monochrome Camera: Orca flash 16 bit data depth 4.2 Megapixel monochrome sCMOS camera.
Color Camera: Hitachi HV-F203SCL 3CCD progresive scan 12bit RGB camera.
Filtercombinations:
| Example Dyes | LED Excitation (Filter set) | Emission Filter set |
| DAPI, FITC, TRITC, Cy5 | 385, 475, 555, 630 (QBS 405 + 493 + 575 + 653) | QBP 425/30 + 514/30 + 592/30 + 709/100 |
| DAPI, FITC, AF594, Cy7 | 385, 475, 590, 735 (QBS 405 + 493 + 611 + 762) | QBP 425/30 + 524/51 + 634/38 + 785/38 |
| CFP, mCherry | 430, 590 ( TBS 450 + 538 + 610) | TBP 467/24 + 555/25 + 687/145 |
| Alexa Fluor 555 | 555 (BS 573) | BP 595/31 |
| Alexa Flour 647 | 630 (BS 652) | BP 676/29 |
Objectives:
| Magnification | Numerical Aperture | Working distance (mm) | Immersion |
|---|---|---|---|
| Fluar 5x | 0.25 | 12,5 | air |
| PApo 10x | 0.45 | 2,1 | air |
| EC Plan-Neofluar 20x | 0.5 | 2,0 | air |
| PApo (Korr) 40x | 0.95 | 0,25 | air |
Software: ZEN Blue
Application: A fully automated slide scanner with a maximum capacity of 25x4 slides. Applicable for fluorescence and transmitted light purposes.