P1: targeting neutrophil subsets

Targeting neutrophil subsets

In repAMI, neutrophils are the first immune cells to enter the injured myocardium. Several, yet incompletely defined subsets have been described in the reperfused myocardium, but their precise localization, their specific tasks and whether their activities can be modified remains incompletely studied. To address this question, the working group is using an established analysis pipeline that allows for the spatiotemporal evaluation of neutrophil subsets in repAMI, human sample and acute myocardial inflammation.

 

Objectives

  • 3D assessment of neutrophil subsets including N1/CD206+N2 neutrophils, pro-angiogenic neutrophils, neutrophils with less extracellular trap formation (OLFM4+), aged neutrophils (CXCR4hi, CD62Llo), reverse-migrated neutrophils (ICAM1hi, CXCR1lo), activated neutrophils (CD64+), and immature neutrophils
  • Functional assessment including migration of neutrophil subsets
  • Regulation of ROS formation in neutrophils through regulation of glucose/lactate metabolism
  • Consequences of selective subset depletion

​      P1_1
3D neutrophil landscape in experimental repAMI

 

Principal Investigators

​Totzeck_Sickmann
​If you are interested in joining this project, please see our available positions here.